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The map label for this gene is dhfrVI [H]

Identifier: 218709871

GI number: 218709871

Start: 2028579

End: 2029130

Strand: Reverse

Name: dhfrVI [H]

Synonym: VS_1884

Alternate gene names: 218709871

Gene position: 2029130-2028579 (Counterclockwise)

Preceding gene: 218709872

Following gene: 218709870

Centisome position: 61.5

GC content: 39.67

Gene sequence:

>552_bases
TTGGTATGGAGTTCAGTGCGCTTGGCTGGCTCGGTGCTGGTGCACCTTATGCCCGGCGTTAGCCCTATGGAGATAAATTT
GAAATTATCATTGATGGCAGCGGTAGCTGAAAATGGAGTAATTGGCTCTGGCCTGGATATACCTTGGATTGTTAAGGGTG
AGCAGCTGTTGTTTAAGGCAATGACTTTTAATCAATGGTTGCTACTTGGCCGTAAAACTTTTGAATCAATGGGAAAACTG
CCAAACCGTAAATATGCGGTTATTTCTCGTTCTCCATCTACATCGAACGATCCTGATATTTTATATTTCAAAAGTGTTGA
AGACGCCTTGGGTTATTTAGAAACAGTAACCGAGCATGCGTTTGTGTCTGGTGGTGGTCAAATATATAAGTCACTCATTA
CGCAAGTGGACACAATTCATATTTCAAAAATTCATAAACATATCTCTGGTGATATTTTCTTCCCTGAACTACCAAGAGAT
TTGACTCTAGTTTTTGAACAGCGATTTAGTTCAAATATTGATTATACGTACCAAATTTGGCAAAAGGGCTAA

Upstream 100 bases:

>100_bases
TATGAAAAGCTAATCGTTAGCATTAATCAGCTAATCATAGAAACAAACAATATCAAGCAGTAAACGGCTAACAAAGGCAT
CAAGGTGACACTCGGCGGTT

Downstream 100 bases:

>100_bases
CAAGGCGCTAAAGACTGATTCGCGAACGCGTGCCGAACTCGCTAACGCTCAAACATGGCACACGTTCACTCACAGCTTAG
CTTGGCGTTAGCTTAAACAT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type VI [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MVWSSVRLAGSVLVHLMPGVSPMEINLKLSLMAAVAENGVIGSGLDIPWIVKGEQLLFKAMTFNQWLLLGRKTFESMGKL
PNRKYAVISRSPSTSNDPDILYFKSVEDALGYLETVTEHAFVSGGGQIYKSLITQVDTIHISKIHKHISGDIFFPELPRD
LTLVFEQRFSSNIDYTYQIWQKG

Sequences:

>Translated_183_residues
MVWSSVRLAGSVLVHLMPGVSPMEINLKLSLMAAVAENGVIGSGLDIPWIVKGEQLLFKAMTFNQWLLLGRKTFESMGKL
PNRKYAVISRSPSTSNDPDILYFKSVEDALGYLETVTEHAFVSGGGQIYKSLITQVDTIHISKIHKHISGDIFFPELPRD
LTLVFEQRFSSNIDYTYQIWQKG
>Mature_183_residues
MVWSSVRLAGSVLVHLMPGVSPMEINLKLSLMAAVAENGVIGSGLDIPWIVKGEQLLFKAMTFNQWLLLGRKTFESMGKL
PNRKYAVISRSPSTSNDPDILYFKSVEDALGYLETVTEHAFVSGGGQIYKSLITQVDTIHISKIHKHISGDIFFPELPRD
LTLVFEQRFSSNIDYTYQIWQKG

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=160, Percent_Identity=31.25, Blast_Score=84, Evalue=7e-18,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 20521; Mature: 20521

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWSSVRLAGSVLVHLMPGVSPMEINLKLSLMAAVAENGVIGSGLDIPWIVKGEQLLFKA
CCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEEHHHCCCCEECCCCCCEEEECHHHHHHH
MTFNQWLLLGRKTFESMGKLPNRKYAVISRSPSTSNDPDILYFKSVEDALGYLETVTEHA
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHH
FVSGGGQIYKSLITQVDTIHISKIHKHISGDIFFPELPRDLTLVFEQRFSSNIDYTYQIW
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCCEEEEEE
QKG
ECC
>Mature Secondary Structure
MVWSSVRLAGSVLVHLMPGVSPMEINLKLSLMAAVAENGVIGSGLDIPWIVKGEQLLFKA
CCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEEHHHCCCCEECCCCCCEEEECHHHHHHH
MTFNQWLLLGRKTFESMGKLPNRKYAVISRSPSTSNDPDILYFKSVEDALGYLETVTEHA
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHH
FVSGGGQIYKSLITQVDTIHISKIHKHISGDIFFPELPRDLTLVFEQRFSSNIDYTYQIW
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCCEEEEEE
QKG
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1941991 [H]