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The map label for this gene is pheS [H]

Identifier: 218709754

GI number: 218709754

Start: 1887894

End: 1888877

Strand: Reverse

Name: pheS [H]

Synonym: VS_1766

Alternate gene names: 218709754

Gene position: 1888877-1887894 (Counterclockwise)

Preceding gene: 218709755

Following gene: 218709753

Centisome position: 57.25

GC content: 46.44

Gene sequence:

>984_bases
ATGCAACATCTAGAAGAGATCATTGCTAATGCAACGACTGCTATTGATACAGCAGATTCGTTAGTCGCACTTGATGAAGT
GCGAGTTCAGTATTTAGGTAAGAAGGGTGAACTAACTCTTCAACTACAAAGCCTAGGTAAACTTCCACCTGAAGAGCGTC
GCACTGCTGGTCAAGAGATCAACAAAGCGAAAGGTGCTGTTCAACAAGCGATCGCAGCTCGCAAAGACGCACTACAACGT
GCAGAGCTTGAAGCGAAACTGGCTGAAGAAACTATCGATGTGAGCCTACCAGGTCGTCGCATTGAGAACGGTGGTCTTCA
CCCAGTTACTCGCACAGTTGAGCGTATCGAACAGTTCTTTGGTGAGCTTGGCTTTAGCACTGAGTCTGGCCCTGAGATCG
AAGATGCATTCCACAACTTTGACGCACTAAACATCGCAGACGATCACCCAGCTCGTACTGATCACGATACTTTCTTCTTC
AACCCTGATCTAATGCTACGTACGCACACTTCTGGTGTTCAAATCCGTACGATGGAAAACGGCAAACCGCCATTCCGCTT
CATTGCTCCGGGTCGTGTTTACCGTAACGACTACGATCAAACTCACACGCCAATGTTCCACCAAGTGGAAGGTATGTTAG
TTGATGAGAACGTAAACTTCGCACAACTTAAAGGCATTCTTAACGATTTCCTTTGTAACTTCTTTGAAGAAGAAGTTGAA
GTGCGTTTCCGTCCTTCATTCTTCCCGTTCACAGAGCCTTCAGCTGAAGTTGACGTGAAACGTAAAGATGGCAAATGGCT
AGAAGTTCTAGGCTGTGGCATGGTTCACCCTAACGTACTTCGCTCTGTTGGCATCGACCCTGAGAAATACTCTGGTTTTG
CATTCGGTATGGGTGTAGAGCGTCTAACGATGCTTCGTTACGGCGTAAATGACCTTCGTGCGTTCTTCGAGAACGACCTT
CGTTTCCTTAAACAATTCAAGTAA

Upstream 100 bases:

>100_bases
ATTCAATTTGGCATTGGATGCGTGTTTGATTACTATGTGTAGCTATCTAAAAAAACTAATCATCCGATACGGACACTACC
AACTGGTAGATGAGGAAATG

Downstream 100 bases:

>100_bases
TCCGGGGCAGTCGAAACTATGAAATTCAGTGAATCTTGGCTACGCGAGTGGGTTAAACCTGCAATTAACAGCGAAGAGCT
AGCTCACCAAATCACTATGG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MQHLEEIIANATTAIDTADSLVALDEVRVQYLGKKGELTLQLQSLGKLPPEERRTAGQEINKAKGAVQQAIAARKDALQR
AELEAKLAEETIDVSLPGRRIENGGLHPVTRTVERIEQFFGELGFSTESGPEIEDAFHNFDALNIADDHPARTDHDTFFF
NPDLMLRTHTSGVQIRTMENGKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILNDFLCNFFEEEVE
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK

Sequences:

>Translated_327_residues
MQHLEEIIANATTAIDTADSLVALDEVRVQYLGKKGELTLQLQSLGKLPPEERRTAGQEINKAKGAVQQAIAARKDALQR
AELEAKLAEETIDVSLPGRRIENGGLHPVTRTVERIEQFFGELGFSTESGPEIEDAFHNFDALNIADDHPARTDHDTFFF
NPDLMLRTHTSGVQIRTMENGKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILNDFLCNFFEEEVE
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK
>Mature_327_residues
MQHLEEIIANATTAIDTADSLVALDEVRVQYLGKKGELTLQLQSLGKLPPEERRTAGQEINKAKGAVQQAIAARKDALQR
AELEAKLAEETIDVSLPGRRIENGGLHPVTRTVERIEQFFGELGFSTESGPEIEDAFHNFDALNIADDHPARTDHDTFFF
NPDLMLRTHTSGVQIRTMENGKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILNDFLCNFFEEEVE
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=331, Percent_Identity=31.1178247734139, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI4758340, Length=259, Percent_Identity=32.8185328185328, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=73.0886850152905, Blast_Score=508, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI32566635, Length=283, Percent_Identity=33.5689045936396, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32563657, Length=260, Percent_Identity=31.9230769230769, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17508957, Length=260, Percent_Identity=31.9230769230769, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321087, Length=268, Percent_Identity=32.089552238806, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=271, Percent_Identity=27.3062730627306, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI18858079, Length=258, Percent_Identity=31.7829457364341, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17137424, Length=280, Percent_Identity=28.2142857142857, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 37109; Mature: 37109

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHLEEIIANATTAIDTADSLVALDEVRVQYLGKKGELTLQLQSLGKLPPEERRTAGQEI
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCHHHHHHHHHH
NKAKGAVQQAIAARKDALQRAELEAKLAEETIDVSLPGRRIENGGLHPVTRTVERIEQFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHH
GELGFSTESGPEIEDAFHNFDALNIADDHPARTDHDTFFFNPDLMLRTHTSGVQIRTMEN
HHHCCCCCCCCCHHHHHCCCCEECCCCCCCCCCCCCEEEECCCEEEEEECCCEEEEEECC
GKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILNDFLCNFFEEEVE
CCCCEEEECCCHHHCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVE
EEECCCCCCCCCCCCCEEEECCCCCEEEEHCCCCCCHHHHHHCCCCHHHCCCEEECCCHH
RLTMLRYGVNDLRAFFENDLRFLKQFK
HHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQHLEEIIANATTAIDTADSLVALDEVRVQYLGKKGELTLQLQSLGKLPPEERRTAGQEI
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCHHHHHHHHHH
NKAKGAVQQAIAARKDALQRAELEAKLAEETIDVSLPGRRIENGGLHPVTRTVERIEQFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHH
GELGFSTESGPEIEDAFHNFDALNIADDHPARTDHDTFFFNPDLMLRTHTSGVQIRTMEN
HHHCCCCCCCCCHHHHHCCCCEECCCCCCCCCCCCCEEEECCCEEEEEECCCEEEEEECC
GKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILNDFLCNFFEEEVE
CCCCEEEECCCHHHCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVE
EEECCCCCCCCCCCCCEEEECCCCCEEEEHCCCCCCHHHHHHCCCCHHHCCCEEECCCHH
RLTMLRYGVNDLRAFFENDLRFLKQFK
HHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA