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The map label for this gene is folK [H]

Identifier: 218709736

GI number: 218709736

Start: 1870918

End: 1871430

Strand: Direct

Name: folK [H]

Synonym: VS_1748

Alternate gene names: 218709736

Gene position: 1870918-1871430 (Clockwise)

Preceding gene: 218709734

Following gene: 218709737

Centisome position: 56.71

GC content: 47.17

Gene sequence:

>513_bases
ATGGCCAACGTCTACGTCAGCATCGGAAGCAACATTAACCGCGAACACCACGTCACAGAATCTCTCAAAGCATTGAATGA
CCGCTTTGCACCACTGCACATCTCTAATTTTTACGATTGCGAGCCTGTCGGTTTCGAAGGGGATAACTTCTTAAACCTTG
TCGTTGGATTCGAATGTAACCTTCCTGTCGCCGAACTGATTCAGGTTCTACATCAAATCGAAGCAGAGAATGATCGCAAG
CGTCAGACCAAAGCCTATGCTTCACGCACGATGGATATCGACATCCTTCTTTATGGTAATCAAGTCGGCATTATTGATGG
CGTAGAGCTGCCTAGAGGCGAAATCACCGAGTACGCATTTGTACTCAGACCGTTAGTCGATATTGCCGCGCAAGAACGCC
ACCCCACTCTAGACATCTCATTTCAGCAGCTCTGGGAAAGCTTCGATCAATTGAGCCAAAAAACCGAACCCATCCCTTTC
GATATCAGTTTCACTCAGCCTTTCCATGACTGA

Upstream 100 bases:

>100_bases
AGAACCCTATTTTCTTTTATCTTCCTGTTTTTAAGACGATGCATAGCTGATCTTCATCGTGAGTTGGACGTATAGGTTTC
TTAAACAAAAAGGAAATACT

Downstream 100 bases:

>100_bases
AAAAAGAACGGCTACAAAATGAACAACGGCTAAAGGAACGACTGAACTCTTAATCACCTGAGCTTTACTGATCTTTTCTT
AAAACAAGCACGTAACTGAC

Product: 7, 8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MANVYVSIGSNINREHHVTESLKALNDRFAPLHISNFYDCEPVGFEGDNFLNLVVGFECNLPVAELIQVLHQIEAENDRK
RQTKAYASRTMDIDILLYGNQVGIIDGVELPRGEITEYAFVLRPLVDIAAQERHPTLDISFQQLWESFDQLSQKTEPIPF
DISFTQPFHD

Sequences:

>Translated_170_residues
MANVYVSIGSNINREHHVTESLKALNDRFAPLHISNFYDCEPVGFEGDNFLNLVVGFECNLPVAELIQVLHQIEAENDRK
RQTKAYASRTMDIDILLYGNQVGIIDGVELPRGEITEYAFVLRPLVDIAAQERHPTLDISFQQLWESFDQLSQKTEPIPF
DISFTQPFHD
>Mature_169_residues
ANVYVSIGSNINREHHVTESLKALNDRFAPLHISNFYDCEPVGFEGDNFLNLVVGFECNLPVAELIQVLHQIEAENDRKR
QTKAYASRTMDIDILLYGNQVGIIDGVELPRGEITEYAFVLRPLVDIAAQERHPTLDISFQQLWESFDQLSQKTEPIPFD
ISFTQPFHD

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=158, Percent_Identity=32.2784810126582, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 19421; Mature: 19290

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVYVSIGSNINREHHVTESLKALNDRFAPLHISNFYDCEPVGFEGDNFLNLVVGFECN
CCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEEEEEECC
LPVAELIQVLHQIEAENDRKRQTKAYASRTMDIDILLYGNQVGIIDGVELPRGEITEYAF
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEEECCEECEEECCCCCCCHHHHHHH
VLRPLVDIAAQERHPTLDISFQQLWESFDQLSQKTEPIPFDISFTQPFHD
HHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
ANVYVSIGSNINREHHVTESLKALNDRFAPLHISNFYDCEPVGFEGDNFLNLVVGFECN
CEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEEEEEECC
LPVAELIQVLHQIEAENDRKRQTKAYASRTMDIDILLYGNQVGIIDGVELPRGEITEYAF
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEEECCEECEEECCCCCCCHHHHHHH
VLRPLVDIAAQERHPTLDISFQQLWESFDQLSQKTEPIPFDISFTQPFHD
HHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1325970; 8202364; 9278503; 1657875; 2537812; 10378268; 10452528 [H]