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The map label for this gene is mdeA [H]

Identifier: 218709732

GI number: 218709732

Start: 1866514

End: 1868325

Strand: Reverse

Name: mdeA [H]

Synonym: VS_1743

Alternate gene names: 218709732

Gene position: 1868325-1866514 (Counterclockwise)

Preceding gene: 218709735

Following gene: 218709730

Centisome position: 56.63

GC content: 47.68

Gene sequence:

>1812_bases
ATGAATACTTCAACTCAACTTAGCCCGCTGCGTAAAACAACCAAACATGAACAAGCAGAAGCCCTTGCCATTGAGCAGGC
AAAGCATTTTGGTATCGACCCAAACAGTGATTACGGCGTCACGCTTATCGAACTGGCGACGACACTGTACAAAGCCAATA
CCAAGACACACGATCTTTGGGCATTGACGGTTGATGGCCTTTCAGAGCTCGATAAGAGCGACCGAATCGCTTGGTTTAAC
GCCAAACGTTTTTTGTCATTCCAGATCGCTAAGATCCTCGACAATCTGCAAAACCCAATGCGCGCCACTTACCAGTCTAT
TGCCACCAATAATGGTAATTTTGCATCCAAAGGTGCGTATCCTATCTTCGATAATGTCGCTGCTATTTTCTCTGCGAGCC
CTGTGATTACACGTACCGCGACCTATTTGTTTGCGTGTACAGAATGGATTGAAGATGCGTTTAATGGTAAGGAGCCGCTG
CACGATATTTACTCTCGACTGCTTAACCCAACATCGATTTCACTGGCTAACCACATGGTTGATATTGAGGCGGGCTCTAG
AGCTAATGAGTACCTCGCATGGAACTTTAACTCGGGGATGGCCGCTATTGATGGGTTGTTGAGCCATCTACTTGGGTATG
AAGACATTGTCTTAGCTTCACGCAATATCTACGGCGGTTCTTACCAACTGTTGGAAGATTGGTTTGGCAAGCCTTCGAAC
CTGAATGTCGCGGTAGAGTGGGTGGATGGTTACTCCGGGGATGAGTTTGCGACGCGACTTGATGAGGTTGCCGATAAATA
CGCGGATCGCATCGCTGCGGGTAAGAAAATCTACGTCTACCTAGAGTCACCGTGTAACCCGCATGGATACGTGTTAGATG
TTGCTAGTATCAGTAAAGCAGGTCACTCTCGTGGTTGGGACGTGATTGTTGACTCGACAGTGGGCACGCCTTTATTACAC
CCAGTACTGAAACGTGATGATGTGATGGAAAGACCCGATTATGTTATTCACTCTTACACCAAAGAACTGGCGGGTTCTGG
CACCACAACTGCTGGGGTCGTGATTGGCCGTAACGAGACCATGTTTATTCCAAAAGGAGAGGAGGTCACTTTCACCAAAC
CTAACGGCGATGCGGCCACCATTCCATGGAACGAAACGCTGTTTTGGAATGTGTACTACATTAAGGGCGCATTCCTAGAC
GCAGACAAAGCGTTTGAAGTGCTTAATGGCATGAAAACGTATGAGATGCGAGTGGTGCAAAAAACGATTAATACACTGAC
TCTCGCGAAGATCTTTGATGCTCACCCAGACATCAATGTGTCGTGTCCAGCTTTGCCAGACAGCGATAACTATGAACACT
GCCAGAACAACATGTACTTGGGTTTACCAGCGGCGCTTTTCACAATCGATATGGAAGGTAACGGCGACCGTGCACCAATC
AATCGAGATGGGTTTAAACAGTTCTTTGACATGCTTGAGCCATCAATCGGCATGCAAGTGAGCTTAGGGCAAACCAACAC
CGTGGCCTTGTGCCCAGCACTGACCACGCACTCAGAGCTCAGCGATGAAGCGTTAAATGAAGCAGGCATCAAGCCAACCA
CAATGCGTATCTCTATCGGCTTGGAAGATCCGCGAATGTTCATCGCTCACATTATCGAGGCCGCTAAATTGTCGATTGAC
CGCAAACACGCAGACTTCTCATCGAGTTTCCCGAGAGGTGGGAGTATCGATGAAATCTATATGCAAACCTATATGGATGT
ACACCAGAGGTTTGTGAAGAGCTTGCCGAAGTTTGAGCAACTTACTCAGTAA

Upstream 100 bases:

>100_bases
TGCTCAAAGTGTATGTCTTACTTCGCAAATAGCAATTTTTAAAATCCGCGCAAAACTAAAATTAAAGGAGGAACAACCAG
CATGGAATGACTTAGGAATT

Downstream 100 bases:

>100_bases
GCTGAGTTATTAAGGAAAGGTGACAATCACTTATTTGGTATACGGTGGCCACTGTTGTAGTCCAGTCATGACACTTAGGG
GGATGTAAAAATGCCTGCTA

Product: putative Cys/Met metabolism pyridoxal-phosphate-dependent enzyme

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MNTSTQLSPLRKTTKHEQAEALAIEQAKHFGIDPNSDYGVTLIELATTLYKANTKTHDLWALTVDGLSELDKSDRIAWFN
AKRFLSFQIAKILDNLQNPMRATYQSIATNNGNFASKGAYPIFDNVAAIFSASPVITRTATYLFACTEWIEDAFNGKEPL
HDIYSRLLNPTSISLANHMVDIEAGSRANEYLAWNFNSGMAAIDGLLSHLLGYEDIVLASRNIYGGSYQLLEDWFGKPSN
LNVAVEWVDGYSGDEFATRLDEVADKYADRIAAGKKIYVYLESPCNPHGYVLDVASISKAGHSRGWDVIVDSTVGTPLLH
PVLKRDDVMERPDYVIHSYTKELAGSGTTTAGVVIGRNETMFIPKGEEVTFTKPNGDAATIPWNETLFWNVYYIKGAFLD
ADKAFEVLNGMKTYEMRVVQKTINTLTLAKIFDAHPDINVSCPALPDSDNYEHCQNNMYLGLPAALFTIDMEGNGDRAPI
NRDGFKQFFDMLEPSIGMQVSLGQTNTVALCPALTTHSELSDEALNEAGIKPTTMRISIGLEDPRMFIAHIIEAAKLSID
RKHADFSSSFPRGGSIDEIYMQTYMDVHQRFVKSLPKFEQLTQ

Sequences:

>Translated_603_residues
MNTSTQLSPLRKTTKHEQAEALAIEQAKHFGIDPNSDYGVTLIELATTLYKANTKTHDLWALTVDGLSELDKSDRIAWFN
AKRFLSFQIAKILDNLQNPMRATYQSIATNNGNFASKGAYPIFDNVAAIFSASPVITRTATYLFACTEWIEDAFNGKEPL
HDIYSRLLNPTSISLANHMVDIEAGSRANEYLAWNFNSGMAAIDGLLSHLLGYEDIVLASRNIYGGSYQLLEDWFGKPSN
LNVAVEWVDGYSGDEFATRLDEVADKYADRIAAGKKIYVYLESPCNPHGYVLDVASISKAGHSRGWDVIVDSTVGTPLLH
PVLKRDDVMERPDYVIHSYTKELAGSGTTTAGVVIGRNETMFIPKGEEVTFTKPNGDAATIPWNETLFWNVYYIKGAFLD
ADKAFEVLNGMKTYEMRVVQKTINTLTLAKIFDAHPDINVSCPALPDSDNYEHCQNNMYLGLPAALFTIDMEGNGDRAPI
NRDGFKQFFDMLEPSIGMQVSLGQTNTVALCPALTTHSELSDEALNEAGIKPTTMRISIGLEDPRMFIAHIIEAAKLSID
RKHADFSSSFPRGGSIDEIYMQTYMDVHQRFVKSLPKFEQLTQ
>Mature_603_residues
MNTSTQLSPLRKTTKHEQAEALAIEQAKHFGIDPNSDYGVTLIELATTLYKANTKTHDLWALTVDGLSELDKSDRIAWFN
AKRFLSFQIAKILDNLQNPMRATYQSIATNNGNFASKGAYPIFDNVAAIFSASPVITRTATYLFACTEWIEDAFNGKEPL
HDIYSRLLNPTSISLANHMVDIEAGSRANEYLAWNFNSGMAAIDGLLSHLLGYEDIVLASRNIYGGSYQLLEDWFGKPSN
LNVAVEWVDGYSGDEFATRLDEVADKYADRIAAGKKIYVYLESPCNPHGYVLDVASISKAGHSRGWDVIVDSTVGTPLLH
PVLKRDDVMERPDYVIHSYTKELAGSGTTTAGVVIGRNETMFIPKGEEVTFTKPNGDAATIPWNETLFWNVYYIKGAFLD
ADKAFEVLNGMKTYEMRVVQKTINTLTLAKIFDAHPDINVSCPALPDSDNYEHCQNNMYLGLPAALFTIDMEGNGDRAPI
NRDGFKQFFDMLEPSIGMQVSLGQTNTVALCPALTTHSELSDEALNEAGIKPTTMRISIGLEDPRMFIAHIIEAAKLSID
RKHADFSSSFPRGGSIDEIYMQTYMDVHQRFVKSLPKFEQLTQ

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1790375, Length=422, Percent_Identity=28.6729857819905, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323333, Length=453, Percent_Identity=27.3730684326711, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6319307, Length=400, Percent_Identity=23.75, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 67038; Mature: 67038

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSTQLSPLRKTTKHEQAEALAIEQAKHFGIDPNSDYGVTLIELATTLYKANTKTHDLW
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEE
ALTVDGLSELDKSDRIAWFNAKRFLSFQIAKILDNLQNPMRATYQSIATNNGNFASKGAY
EEEECCHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PIFDNVAAIFSASPVITRTATYLFACTEWIEDAFNGKEPLHDIYSRLLNPTSISLANHMV
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEHHHHEE
DIEAGSRANEYLAWNFNSGMAAIDGLLSHLLGYEDIVLASRNIYGGSYQLLEDWFGKPSN
EECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCC
LNVAVEWVDGYSGDEFATRLDEVADKYADRIAAGKKIYVYLESPCNPHGYVLDVASISKA
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEHHHHHHC
GHSRGWDVIVDSTVGTPLLHPVLKRDDVMERPDYVIHSYTKELAGSGTTTAGVVIGRNET
CCCCCCEEEEECCCCCHHHHHHHHCCCHHCCCCHHHHHHHHHHCCCCCCEEEEEEECCCE
MFIPKGEEVTFTKPNGDAATIPWNETLFWNVYYIKGAFLDADKAFEVLNGMKTYEMRVVQ
EEEECCCEEEEECCCCCEEECCCCCEEEEEEEEEEEEEECHHHHHHHHCCCHHHHHHHHH
KTINTLTLAKIFDAHPDINVSCPALPDSDNYEHCQNNMYLGLPAALFTIDMEGNGDRAPI
HHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHCCEEEECCEEEEEEEECCCCCCCCC
NRDGFKQFFDMLEPSIGMQVSLGQTNTVALCPALTTHSELSDEALNEAGIKPTTMRISIG
CHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEEC
LEDPRMFIAHIIEAAKLSIDRKHADFSSSFPRGGSIDEIYMQTYMDVHQRFVKSLPKFEQ
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
LTQ
HCC
>Mature Secondary Structure
MNTSTQLSPLRKTTKHEQAEALAIEQAKHFGIDPNSDYGVTLIELATTLYKANTKTHDLW
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEE
ALTVDGLSELDKSDRIAWFNAKRFLSFQIAKILDNLQNPMRATYQSIATNNGNFASKGAY
EEEECCHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PIFDNVAAIFSASPVITRTATYLFACTEWIEDAFNGKEPLHDIYSRLLNPTSISLANHMV
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEHHHHEE
DIEAGSRANEYLAWNFNSGMAAIDGLLSHLLGYEDIVLASRNIYGGSYQLLEDWFGKPSN
EECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCC
LNVAVEWVDGYSGDEFATRLDEVADKYADRIAAGKKIYVYLESPCNPHGYVLDVASISKA
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEHHHHHHC
GHSRGWDVIVDSTVGTPLLHPVLKRDDVMERPDYVIHSYTKELAGSGTTTAGVVIGRNET
CCCCCCEEEEECCCCCHHHHHHHHCCCHHCCCCHHHHHHHHHHCCCCCCEEEEEEECCCE
MFIPKGEEVTFTKPNGDAATIPWNETLFWNVYYIKGAFLDADKAFEVLNGMKTYEMRVVQ
EEEECCCEEEEECCCCCEEECCCCCEEEEEEEEEEEEEECHHHHHHHHCCCHHHHHHHHH
KTINTLTLAKIFDAHPDINVSCPALPDSDNYEHCQNNMYLGLPAALFTIDMEGNGDRAPI
HHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHCCEEEECCEEEEEEEECCCCCCCCC
NRDGFKQFFDMLEPSIGMQVSLGQTNTVALCPALTTHSELSDEALNEAGIKPTTMRISIG
CHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEEC
LEDPRMFIAHIIEAAKLSIDRKHADFSSSFPRGGSIDEIYMQTYMDVHQRFVKSLPKFEQ
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
LTQ
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]