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The map label for this gene is cqsA [H]

Identifier: 218709713

GI number: 218709713

Start: 1850885

End: 1852066

Strand: Direct

Name: cqsA [H]

Synonym: VS_1724

Alternate gene names: 218709713

Gene position: 1850885-1852066 (Clockwise)

Preceding gene: 218709712

Following gene: 218709716

Centisome position: 56.1

GC content: 47.21

Gene sequence:

>1182_bases
ATGAGTGATACAACAAAAAACAAACCACTACCTTCCTTTATCGAAGAACGCTTGAATTTCTATATTCAAGACCTCATTAC
CCAGAACCAAAGCCAAAAGCACTTGGTATTGGGTAAACGACCTCAGCGCAATGCCGTCGTGATGCAGAGCAATGATTACT
TAGCACTGTCACACAACAAACCGATCCAAGACGCCCATCAAGCAGCGATTATTGAACACGATGACAACGTAGTGATGTCT
GCAATTTTTTTACAAGATGAAGAGTCTAAACCTGCGTTTGAAACCGAGCTCGCGAACTATGTCGGAATGGAGAGCTGTTT
GCTTTCTCAGTCAGGTTGGGCGGCCAATATTGGACTATTACAAACCATCTGCCCGCCGGACACATCGGTGTATATCGACT
TTTTTGCCCATATGTCGCTATGGGAAGGCATTCGTGCCGCTGGCGGCATCGCCCACCCGTTTATGCATAACAATATGAAT
CACCTGCGTAAACAGTTAGAGCGCCACGGCTCAGGCGTTATTGTGGTCGATTCGGTTTACAGTACCATTGGGACCATCGC
CCCACTACGCGACATCTACGAAATGGCGCAGGAGTTTGATTGTGCCGTCGTCGTTGATGAATCGCATTCATTGGGCACGC
ATGGGGAAAATGGTGCCGGCATAGTTCACGCATTGGGCCTTACAAAGCAAGTCGACTTCATCACCGTAAGTTTGGCTAAA
ACCTTTGCTTATCGTGCAGGCGCGATCCTTGGACCAAAACTACTTTCAGAAACCTTACCGTTTGTTGCTTATCCCGCGAT
TTTCAGCTCGACCGTGTTACCACAAGAGGTGGTCCGTTTAGAGAAAACATTAGAGGTAATCAAAAGTGCAGATGATAAAC
GACAGGCTCTGTTCCAGCGTGCTAAATCGTTGACTACGGGTCTAAAACGAATTGGCTTTAATATCCGCAGCGAGTCTCAA
ATTGTGGCATTAGAATGTGGTAGTGAAAGAAACACTGAACGTGTTCGCGATTTCCTCGAAGAACGGAATGTGTTCGGTGC
GGTGTTCTGCCGCCCCGCTACGGGCAAGAACAAGAACATCATCCGATTTTCAGTGAATGCCGATATGACACCACGAGACA
TTGACCATGTGCTCACCGTTTGTCACGAAGCGTACCATCACCCAGAGTTAGAGTTCGTATAA

Upstream 100 bases:

>100_bases
CACATACAAAATTATGCAAACTGACTAATGACACATCACTTTTTAACTTCAATATACAACCGCCGGCGCTGGCGTTTTCC
CGTATTTGAAGAGAATAATT

Downstream 100 bases:

>100_bases
CTCTGTACACACCAATTCTATAACTAACACAAACGGCAGGACTGAATAGACGCTATCATGTCGCGATACAAAAAGAGCCC
AGCGAATTGCTGAGCTCTTT

Product: hypothetical protein

Products: Acetyl-CoA; Glycine [C]

Alternate protein names: Cholerae quorum-sensing autoinducer [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSDTTKNKPLPSFIEERLNFYIQDLITQNQSQKHLVLGKRPQRNAVVMQSNDYLALSHNKPIQDAHQAAIIEHDDNVVMS
AIFLQDEESKPAFETELANYVGMESCLLSQSGWAANIGLLQTICPPDTSVYIDFFAHMSLWEGIRAAGGIAHPFMHNNMN
HLRKQLERHGSGVIVVDSVYSTIGTIAPLRDIYEMAQEFDCAVVVDESHSLGTHGENGAGIVHALGLTKQVDFITVSLAK
TFAYRAGAILGPKLLSETLPFVAYPAIFSSTVLPQEVVRLEKTLEVIKSADDKRQALFQRAKSLTTGLKRIGFNIRSESQ
IVALECGSERNTERVRDFLEERNVFGAVFCRPATGKNKNIIRFSVNADMTPRDIDHVLTVCHEAYHHPELEFV

Sequences:

>Translated_393_residues
MSDTTKNKPLPSFIEERLNFYIQDLITQNQSQKHLVLGKRPQRNAVVMQSNDYLALSHNKPIQDAHQAAIIEHDDNVVMS
AIFLQDEESKPAFETELANYVGMESCLLSQSGWAANIGLLQTICPPDTSVYIDFFAHMSLWEGIRAAGGIAHPFMHNNMN
HLRKQLERHGSGVIVVDSVYSTIGTIAPLRDIYEMAQEFDCAVVVDESHSLGTHGENGAGIVHALGLTKQVDFITVSLAK
TFAYRAGAILGPKLLSETLPFVAYPAIFSSTVLPQEVVRLEKTLEVIKSADDKRQALFQRAKSLTTGLKRIGFNIRSESQ
IVALECGSERNTERVRDFLEERNVFGAVFCRPATGKNKNIIRFSVNADMTPRDIDHVLTVCHEAYHHPELEFV
>Mature_392_residues
SDTTKNKPLPSFIEERLNFYIQDLITQNQSQKHLVLGKRPQRNAVVMQSNDYLALSHNKPIQDAHQAAIIEHDDNVVMSA
IFLQDEESKPAFETELANYVGMESCLLSQSGWAANIGLLQTICPPDTSVYIDFFAHMSLWEGIRAAGGIAHPFMHNNMNH
LRKQLERHGSGVIVVDSVYSTIGTIAPLRDIYEMAQEFDCAVVVDESHSLGTHGENGAGIVHALGLTKQVDFITVSLAKT
FAYRAGAILGPKLLSETLPFVAYPAIFSSTVLPQEVVRLEKTLEVIKSADDKRQALFQRAKSLTTGLKRIGFNIRSESQI
VALECGSERNTERVRDFLEERNVFGAVFCRPATGKNKNIIRFSVNADMTPRDIDHVLTVCHEAYHHPELEFV

Specific function: Required for the synthesis of the quorum-sensing autoinducer CAI-1 ((S)-3-hydroxytridecan-4-one) which probably functions as an intragenus signal [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=343, Percent_Identity=28.5714285714286, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI284448556, Length=343, Percent_Identity=28.5714285714286, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI83977442, Length=342, Percent_Identity=24.2690058479532, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI83977440, Length=342, Percent_Identity=24.2690058479532, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI83977444, Length=342, Percent_Identity=24.2690058479532, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI4502025, Length=352, Percent_Identity=25.5681818181818, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI40316939, Length=352, Percent_Identity=25.5681818181818, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4758668, Length=358, Percent_Identity=24.3016759776536, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI119220554, Length=313, Percent_Identity=25.8785942492013, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1790046, Length=332, Percent_Identity=26.5060240963855, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786993, Length=346, Percent_Identity=26.5895953757225, Blast_Score=111, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71994529, Length=336, Percent_Identity=24.1071428571429, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI32566772, Length=353, Percent_Identity=21.8130311614731, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560912, Length=363, Percent_Identity=23.1404958677686, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17560914, Length=363, Percent_Identity=23.1404958677686, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71982625, Length=306, Percent_Identity=22.8758169934641, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71982617, Length=306, Percent_Identity=22.8758169934641, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320267, Length=306, Percent_Identity=23.8562091503268, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6320438, Length=342, Percent_Identity=25.1461988304094, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323954, Length=275, Percent_Identity=21.0909090909091, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24662918, Length=369, Percent_Identity=25.4742547425474, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17137420, Length=316, Percent_Identity=27.2151898734177, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17136286, Length=324, Percent_Identity=24.6913580246914, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24653280, Length=229, Percent_Identity=20.5240174672489, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24653276, Length=229, Percent_Identity=20.5240174672489, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24653278, Length=229, Percent_Identity=20.5240174672489, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.3.1.29 [C]

Molecular weight: Translated: 43833; Mature: 43702

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00559 MOLYBDOPTERIN_EUK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTTKNKPLPSFIEERLNFYIQDLITQNQSQKHLVLGKRPQRNAVVMQSNDYLALSHNK
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEECCCEEEECCCC
PIQDAHQAAIIEHDDNVVMSAIFLQDEESKPAFETELANYVGMESCLLSQSGWAANIGLL
CCHHHHHEEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCHH
QTICPPDTSVYIDFFAHMSLWEGIRAAGGIAHPFMHNNMNHLRKQLERHGSGVIVVDSVY
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCCCEEEEHHHH
STIGTIAPLRDIYEMAQEFDCAVVVDESHSLGTHGENGAGIVHALGLTKQVDFITVSLAK
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCHHHEEHHHHH
TFAYRAGAILGPKLLSETLPFVAYPAIFSSTVLPQEVVRLEKTLEVIKSADDKRQALFQR
HHHHHCCCCCCHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
AKSLTTGLKRIGFNIRSESQIVALECGSERNTERVRDFLEERNVFGAVFCRPATGKNKNI
HHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCE
IRFSVNADMTPRDIDHVLTVCHEAYHHPELEFV
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDTTKNKPLPSFIEERLNFYIQDLITQNQSQKHLVLGKRPQRNAVVMQSNDYLALSHNK
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEECCCEEEECCCC
PIQDAHQAAIIEHDDNVVMSAIFLQDEESKPAFETELANYVGMESCLLSQSGWAANIGLL
CCHHHHHEEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCHH
QTICPPDTSVYIDFFAHMSLWEGIRAAGGIAHPFMHNNMNHLRKQLERHGSGVIVVDSVY
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCCCEEEEHHHH
STIGTIAPLRDIYEMAQEFDCAVVVDESHSLGTHGENGAGIVHALGLTKQVDFITVSLAK
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCHHHEEHHHHH
TFAYRAGAILGPKLLSETLPFVAYPAIFSSTVLPQEVVRLEKTLEVIKSADDKRQALFQR
HHHHHCCCCCCHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
AKSLTTGLKRIGFNIRSESQIVALECGSERNTERVRDFLEERNVFGAVFCRPATGKNKNI
HHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCE
IRFSVNADMTPRDIDHVLTVCHEAYHHPELEFV
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5-phosphate mol per mol subunit, enzyme, [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 12 {butyryl-CoA}} 0.08 {propionyl-CoA}} 0.059 {acetyl-CoA}} [C]

Substrates: L-2-Amino-3-oxobutanoate; Coenzyme A [C]

Specific reaction: L-2-Amino-3-oxobutanoate + Coenzyme A --> Acetyl-CoA + Glycine [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 2-Cyclohexanedione; 2, 3-Butanedione; 4-(Oxoacetyl) phenoxyacetic acid; Aminomalonic acid; Aminomethyl phosphonic acid; Aminooxyacetate; Ba2+; Ca2+; Cd2+; CoA; Cu2+; DTT; Glutathione; Hg2+; Hydroxy l amine; IAA; L-Cysteine; Mn2+; PCMB, NEM, glycinemeth

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA