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The map label for this gene is proW [H]

Identifier: 218709515

GI number: 218709515

Start: 1659293

End: 1660192

Strand: Direct

Name: proW [H]

Synonym: VS_1524

Alternate gene names: 218709515

Gene position: 1659293-1660192 (Clockwise)

Preceding gene: 218709514

Following gene: 218709516

Centisome position: 50.29

GC content: 46.11

Gene sequence:

>900_bases
ATGGCTGACACCAATTGGTTATCAACCTTTCCAGAGATGGAACGCGCTGATTTACGAACCATAAAAAAGACGCTAGATGG
CGCATACCGTGAATTCTCTCGTGAATACGGTGAAATGATTGAATCTCTATTTGACCCTCTTCTTTCATTCCTGGTTTGGT
TTGAAAAACTTCTTATCTCAACCCCTTGGATTATAGTCCTCGCTGTTTGTACAAGCCTTGTCTACGCCGCGAGTCGTTCT
TGGAAACTGGCTTTAGGTTGTGTCGTTTCCCTGCTCCTTATAGGTTACTTTGGTATGTGGGAAGACACCATGCGTACGCT
CAGTATCATCACTGTGTGTACCTTGGTTTCGATATTCTTAGGCATTCCGATTGGCATTGCGATGGCTCGCTCGAATCGAG
CGCAATCTATCGTTACGCCGATGCTTGATATCATGCAAACCATGCCAGCATTCGTTTACTTGATCCCCGTGGTAATGCTA
CTCGGCATCGGTAAGATTCCAGGGCTGATCGCCGTGGTTATCTACGCGATTCCACCTGTGATTCGTTTAACCAACCTAGG
CATACGCTTAGTCGACAAAGAAGTATTGGAAGCGGCTACCGCTTTTGGTGCGAGCAAAAAGCAACGCTTATGGGGTGTTC
AACTACCGTTAGCAATGCCAACGATCATGGCGGGTATCAACCAAACCATCATGATGGCACTGTCTATGGTTGTTATCGCA
TCAATGATTGGTGTTAAAGGCCTAGGACAACCGGTTCTTAAATCTATTACCAACCAATACTTCACATTAGGCTTGATGAA
TGGTTTCGCCATTGTTGCCCTAGCAATCCTTTTTGACCGCGCTTCGCAGGCGTACGCAAGAAGAACCAATGCGCATTTAG
GAGGACTCAAGCATGACTAA

Upstream 100 bases:

>100_bases
ATAACGTCGCTATACTTGATGTTATTCATGATTCGGAGCCCTTTTCATAGGCGATTACCTCATCGTATGAACAAGTGGCT
CCGAACTATAAGGATATAAA

Downstream 100 bases:

>100_bases
ACCATTGATTGAGATTAGTGGCCTATACAAAGTGTTTGGACCGAAACCGATGTCTGTTATGAACCGAGTTCAGAGCGGCG
AACACAAAGACCAGATTCTT

Product: ABC transporter transmembrane protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MADTNWLSTFPEMERADLRTIKKTLDGAYREFSREYGEMIESLFDPLLSFLVWFEKLLISTPWIIVLAVCTSLVYAASRS
WKLALGCVVSLLLIGYFGMWEDTMRTLSIITVCTLVSIFLGIPIGIAMARSNRAQSIVTPMLDIMQTMPAFVYLIPVVML
LGIGKIPGLIAVVIYAIPPVIRLTNLGIRLVDKEVLEAATAFGASKKQRLWGVQLPLAMPTIMAGINQTIMMALSMVVIA
SMIGVKGLGQPVLKSITNQYFTLGLMNGFAIVALAILFDRASQAYARRTNAHLGGLKHD

Sequences:

>Translated_299_residues
MADTNWLSTFPEMERADLRTIKKTLDGAYREFSREYGEMIESLFDPLLSFLVWFEKLLISTPWIIVLAVCTSLVYAASRS
WKLALGCVVSLLLIGYFGMWEDTMRTLSIITVCTLVSIFLGIPIGIAMARSNRAQSIVTPMLDIMQTMPAFVYLIPVVML
LGIGKIPGLIAVVIYAIPPVIRLTNLGIRLVDKEVLEAATAFGASKKQRLWGVQLPLAMPTIMAGINQTIMMALSMVVIA
SMIGVKGLGQPVLKSITNQYFTLGLMNGFAIVALAILFDRASQAYARRTNAHLGGLKHD
>Mature_298_residues
ADTNWLSTFPEMERADLRTIKKTLDGAYREFSREYGEMIESLFDPLLSFLVWFEKLLISTPWIIVLAVCTSLVYAASRSW
KLALGCVVSLLLIGYFGMWEDTMRTLSIITVCTLVSIFLGIPIGIAMARSNRAQSIVTPMLDIMQTMPAFVYLIPVVMLL
GIGKIPGLIAVVIYAIPPVIRLTNLGIRLVDKEVLEAATAFGASKKQRLWGVQLPLAMPTIMAGINQTIMMALSMVVIAS
MIGVKGLGQPVLKSITNQYFTLGLMNGFAIVALAILFDRASQAYARRTNAHLGGLKHD

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=258, Percent_Identity=46.1240310077519, Blast_Score=210, Evalue=7e-56,
Organism=Escherichia coli, GI1788451, Length=196, Percent_Identity=31.1224489795918, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788449, Length=189, Percent_Identity=30.6878306878307, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1786564, Length=152, Percent_Identity=30.2631578947368, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32909; Mature: 32778

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTNWLSTFPEMERADLRTIKKTLDGAYREFSREYGEMIESLFDPLLSFLVWFEKLLIS
CCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPWIIVLAVCTSLVYAASRSWKLALGCVVSLLLIGYFGMWEDTMRTLSIITVCTLVSIFL
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIPIGIAMARSNRAQSIVTPMLDIMQTMPAFVYLIPVVMLLGIGKIPGLIAVVIYAIPPV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IRLTNLGIRLVDKEVLEAATAFGASKKQRLWGVQLPLAMPTIMAGINQTIMMALSMVVIA
HHHHHCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SMIGVKGLGQPVLKSITNQYFTLGLMNGFAIVALAILFDRASQAYARRTNAHLGGLKHD
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADTNWLSTFPEMERADLRTIKKTLDGAYREFSREYGEMIESLFDPLLSFLVWFEKLLIS
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPWIIVLAVCTSLVYAASRSWKLALGCVVSLLLIGYFGMWEDTMRTLSIITVCTLVSIFL
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIPIGIAMARSNRAQSIVTPMLDIMQTMPAFVYLIPVVMLLGIGKIPGLIAVVIYAIPPV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IRLTNLGIRLVDKEVLEAATAFGASKKQRLWGVQLPLAMPTIMAGINQTIMMALSMVVIA
HHHHHCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SMIGVKGLGQPVLKSITNQYFTLGLMNGFAIVALAILFDRASQAYARRTNAHLGGLKHD
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]