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The map label for this gene is nagK

Identifier: 218709498

GI number: 218709498

Start: 1642075

End: 1642983

Strand: Reverse

Name: nagK

Synonym: VS_1507

Alternate gene names: 218709498

Gene position: 1642983-1642075 (Counterclockwise)

Preceding gene: 218709499

Following gene: 218709496

Centisome position: 49.8

GC content: 45.87

Gene sequence:

>909_bases
ATGTATTACGGCTTCGATGTTGGCGGCACAAAGATTGAGTTTGGTGCATTCAATGAAAAACTTGAGCGAGTAGCGACAGA
ACGCGTACCAACGCCCACTGAAGATTATCAACTGCTACTTGATACAATTGCTGGTTTGGTAAAGAAGTACGACAACGAAT
TTTCTTGCGAAGGCAAAATTGGTCTTGGTCTTCCTGGAATGGAAGATGCTGACGACGGCACAATGCTGGTTGTTAACGTA
CCAGCTTCAACGGGGAAACCACTACGCAAAGACCTAGAAGCGCTTATTGGTCGTAGTGTGAAAATTGAAAATGATGCAAA
CTGTTTTGCGCTTTCAGAAGCGTGGGATGATGAACTTAAAGACGAACCATCAGTAGCAGGCCTAATTCTAGGTACTGGCT
TCGGTGGCGGTTTAGTTTATGAAGGTAAAGTGTTCTCTGGTCGTAATCACGTTGCTGGTGAATTAGGTCACATGCGCCTT
CCTCTAGATGCATGGTTCCACCTTGGCGACAACGCACCGCTACTGGGTTGTGGTTGTGGCAAGAAAGGTTGTTTAGACAG
TTACTTATCGGGTCGCGGCTTCGAACTGATTTATGAGCACTACTTCGGTGAGAAGAAGAAAGCGATCGAGATCATTCAAG
CATACAACGAAGGCGAATCAAAAGCCGCTGAGCACGTAGATCGCTTCATGGAGCTATTGGCTATCTGTTTTGCGAACCTG
TTTACGGGTCTTGACCCACATGTTGTTGCACTGGGTGGTGGTCTTTCAAACTTCGAACTTATCTACGAAGAAATGCCAAA
GCGCATACCTAAGTACCTACTGTCTGTAGCGAAGTGTCCAAAAATCATCAAAGCGAAACACGGTGATTCAGGCGGTGTTC
GTGGCGCTGCATTCCTTAACATTAAGTAA

Upstream 100 bases:

>100_bases
CTATCACGGTTTGTAGGGGGAGTGTTATGGATAATGCTCTTTAGTTGTTTATTTACAAAAATTTATTTGCCCGAATTTAG
GGAAAAATTGGAGAGATTTC

Downstream 100 bases:

>100_bases
CGGTTATTTAATGTTAAGCTAGAGCTAGCTAAAAAGCATCAATAAAAATGCCGCAATCTCGAAAGAGGTTGCGGCATTTT
TTATTGTTCAGTGTTTTCGT

Product: N-acetyl-D-glucosamine kinase

Products: NA

Alternate protein names: GlcNAc kinase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MYYGFDVGGTKIEFGAFNEKLERVATERVPTPTEDYQLLLDTIAGLVKKYDNEFSCEGKIGLGLPGMEDADDGTMLVVNV
PASTGKPLRKDLEALIGRSVKIENDANCFALSEAWDDELKDEPSVAGLILGTGFGGGLVYEGKVFSGRNHVAGELGHMRL
PLDAWFHLGDNAPLLGCGCGKKGCLDSYLSGRGFELIYEHYFGEKKKAIEIIQAYNEGESKAAEHVDRFMELLAICFANL
FTGLDPHVVALGGGLSNFELIYEEMPKRIPKYLLSVAKCPKIIKAKHGDSGGVRGAAFLNIK

Sequences:

>Translated_302_residues
MYYGFDVGGTKIEFGAFNEKLERVATERVPTPTEDYQLLLDTIAGLVKKYDNEFSCEGKIGLGLPGMEDADDGTMLVVNV
PASTGKPLRKDLEALIGRSVKIENDANCFALSEAWDDELKDEPSVAGLILGTGFGGGLVYEGKVFSGRNHVAGELGHMRL
PLDAWFHLGDNAPLLGCGCGKKGCLDSYLSGRGFELIYEHYFGEKKKAIEIIQAYNEGESKAAEHVDRFMELLAICFANL
FTGLDPHVVALGGGLSNFELIYEEMPKRIPKYLLSVAKCPKIIKAKHGDSGGVRGAAFLNIK
>Mature_302_residues
MYYGFDVGGTKIEFGAFNEKLERVATERVPTPTEDYQLLLDTIAGLVKKYDNEFSCEGKIGLGLPGMEDADDGTMLVVNV
PASTGKPLRKDLEALIGRSVKIENDANCFALSEAWDDELKDEPSVAGLILGTGFGGGLVYEGKVFSGRNHVAGELGHMRL
PLDAWFHLGDNAPLLGCGCGKKGCLDSYLSGRGFELIYEHYFGEKKKAIEIIQAYNEGESKAAEHVDRFMELLAICFANL
FTGLDPHVVALGGGLSNFELIYEEMPKRIPKYLLSVAKCPKIIKAKHGDSGGVRGAAFLNIK

Specific function: Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NagK subfamily

Homologues:

Organism=Escherichia coli, GI1787363, Length=302, Percent_Identity=51.6556291390728, Blast_Score=352, Evalue=1e-98,
Organism=Escherichia coli, GI87081733, Length=301, Percent_Identity=37.2093023255814, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1790522, Length=306, Percent_Identity=26.797385620915, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NAGK_VIBSL (B7VNU4)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417119.1
- ProteinModelPortal:   B7VNU4
- GeneID:   7161079
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_1507
- HOGENOM:   HBG730260
- OMA:   GMEDADD
- ProtClustDB:   PRK13310
- HAMAP:   MF_01271
- InterPro:   IPR000600

Pfam domain/function: PF00480 ROK

EC number: =2.7.1.59

Molecular weight: Translated: 32820; Mature: 32820

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYGFDVGGTKIEFGAFNEKLERVATERVPTPTEDYQLLLDTIAGLVKKYDNEFSCEGKI
CEEEECCCCCEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
GLGLPGMEDADDGTMLVVNVPASTGKPLRKDLEALIGRSVKIENDANCFALSEAWDDELK
CCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHHHC
DEPSVAGLILGTGFGGGLVYEGKVFSGRNHVAGELGHMRLPLDAWFHLGDNAPLLGCGCG
CCCCEEEEEEECCCCCCEEEECEEECCCCCCCCCCCCEECCHHHHEECCCCCCEEECCCC
KKGCLDSYLSGRGFELIYEHYFGEKKKAIEIIQAYNEGESKAAEHVDRFMELLAICFANL
CCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FTGLDPHVVALGGGLSNFELIYEEMPKRIPKYLLSVAKCPKIIKAKHGDSGGVRGAAFLN
HCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEE
IK
CC
>Mature Secondary Structure
MYYGFDVGGTKIEFGAFNEKLERVATERVPTPTEDYQLLLDTIAGLVKKYDNEFSCEGKI
CEEEECCCCCEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
GLGLPGMEDADDGTMLVVNVPASTGKPLRKDLEALIGRSVKIENDANCFALSEAWDDELK
CCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHHHC
DEPSVAGLILGTGFGGGLVYEGKVFSGRNHVAGELGHMRLPLDAWFHLGDNAPLLGCGCG
CCCCEEEEEEECCCCCCEEEECEEECCCCCCCCCCCCEECCHHHHEECCCCCCEEECCCC
KKGCLDSYLSGRGFELIYEHYFGEKKKAIEIIQAYNEGESKAAEHVDRFMELLAICFANL
CCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FTGLDPHVVALGGGLSNFELIYEEMPKRIPKYLLSVAKCPKIIKAKHGDSGGVRGAAFLN
HCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEE
IK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA