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The map label for this gene is moaA

Identifier: 218708979

GI number: 218708979

Start: 1018318

End: 1019322

Strand: Direct

Name: moaA

Synonym: VS_0985

Alternate gene names: 218708979

Gene position: 1018318-1019322 (Clockwise)

Preceding gene: 218708977

Following gene: 218708980

Centisome position: 30.86

GC content: 44.68

Gene sequence:

>1005_bases
TTGGAAAGGTGTTCCGTGGCGCAACAATTCGAAGATAGATTCCATCGCAAGTTTTATTACTTGCGCTTGTCGGTTACAGA
CGTCTGTAACTTTAAATGTACTTACTGCTTGCCAGATGGTTATAAACCGTCAGGGCAAAAAAACTCGTCTTTTTTAAGTG
TTCCTGAGATCAAACGCGTTGTTAAAGCGTTTGCTGATTGTGGTACCTCAAAGATCCGCATTACTGGCGGAGAGCCGAGT
CTGCGTAAAGACTTTCCTGAAATCATACATACCGTTGCTTCTACTCCAGGCATCGAAAAAGTAGCCACCACAACGAATGG
CTACCGCATGGAGAAACAAGTAGGGCAATGGCGTGATGCTGGTTTAACCCATATAAACGTGAGCGTGGATAGCTTAGATT
CGCGCATGTTCCATCAGATCACTGGTGAGAACAAGTTTACTGAGGTTATGCGAGGTATTGATAAAGCGTTTGAGGTTGGC
TTTGAACAAGTCAAAGTCAACGTTGTATTAATGAAAGACCTCAACAGTCAGGAATTACCAGCCTTCCTTAATTGGATCAA
AGACAAACCTATTCAATTACGTTTTATCGAGCTGATGAAAACCGGCGAGATGGACGAGTTGTTCGATAAACACCATGTGT
CTGGCGTTGCGATTCGCAACCAGCTTATTGCTAATGGCTGGCTGTTAAAAGTCAAAGCCGTTAATGATGGCCCTGCGCAA
GTGTTTGTCCACCCAGACTACCAGGGTGAAATCGGTTTGATCATGCCTTACGAGAAAGACTTTTGTGAGAGTTGTAACCG
ACTGCGTGTTTCTGCGACCGGTAAACTTCACTTATGTCTGTTTGGCGACCATGGTGTTGAACTGAGAGATCTGATTCAAG
AAGATCAACAAGAGCAAGAGCTCATTGATCGAATTCAGGCTCAGCTTCAAACCAAGTCCGTTAGCCACTTCTTACATGAT
GGCAACAGCGGCATGACTCCAAACTTGGCGTCAATCGGCGGTTAA

Upstream 100 bases:

>100_bases
ATGCTAAGTCGCACAGTGGTATTTGCTGTACCGATACGCATCAAGAGCCAGTGACATGTTGTCACAAGGGCTTAATTGGA
AATGATTATGCCTCCCGTGT

Downstream 100 bases:

>100_bases
CCCGCTATATACTCTAAAGCATTTTATCGCTCAGCTTCTTTAAGCTGGGCGAATAAGTCTAGGGCGTGTTGCTCTTTCGA
GCTGATTTTTGCAGCGAGTT

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MERCSVAQQFEDRFHRKFYYLRLSVTDVCNFKCTYCLPDGYKPSGQKNSSFLSVPEIKRVVKAFADCGTSKIRITGGEPS
LRKDFPEIIHTVASTPGIEKVATTTNGYRMEKQVGQWRDAGLTHINVSVDSLDSRMFHQITGENKFTEVMRGIDKAFEVG
FEQVKVNVVLMKDLNSQELPAFLNWIKDKPIQLRFIELMKTGEMDELFDKHHVSGVAIRNQLIANGWLLKVKAVNDGPAQ
VFVHPDYQGEIGLIMPYEKDFCESCNRLRVSATGKLHLCLFGDHGVELRDLIQEDQQEQELIDRIQAQLQTKSVSHFLHD
GNSGMTPNLASIGG

Sequences:

>Translated_334_residues
MERCSVAQQFEDRFHRKFYYLRLSVTDVCNFKCTYCLPDGYKPSGQKNSSFLSVPEIKRVVKAFADCGTSKIRITGGEPS
LRKDFPEIIHTVASTPGIEKVATTTNGYRMEKQVGQWRDAGLTHINVSVDSLDSRMFHQITGENKFTEVMRGIDKAFEVG
FEQVKVNVVLMKDLNSQELPAFLNWIKDKPIQLRFIELMKTGEMDELFDKHHVSGVAIRNQLIANGWLLKVKAVNDGPAQ
VFVHPDYQGEIGLIMPYEKDFCESCNRLRVSATGKLHLCLFGDHGVELRDLIQEDQQEQELIDRIQAQLQTKSVSHFLHD
GNSGMTPNLASIGG
>Mature_334_residues
MERCSVAQQFEDRFHRKFYYLRLSVTDVCNFKCTYCLPDGYKPSGQKNSSFLSVPEIKRVVKAFADCGTSKIRITGGEPS
LRKDFPEIIHTVASTPGIEKVATTTNGYRMEKQVGQWRDAGLTHINVSVDSLDSRMFHQITGENKFTEVMRGIDKAFEVG
FEQVKVNVVLMKDLNSQELPAFLNWIKDKPIQLRFIELMKTGEMDELFDKHHVSGVAIRNQLIANGWLLKVKAVNDGPAQ
VFVHPDYQGEIGLIMPYEKDFCESCNRLRVSATGKLHLCLFGDHGVELRDLIQEDQQEQELIDRIQAQLQTKSVSHFLHD
GNSGMTPNLASIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=309, Percent_Identity=37.2168284789644, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI22027536, Length=309, Percent_Identity=37.2168284789644, Blast_Score=198, Evalue=5e-51,
Organism=Escherichia coli, GI1786999, Length=329, Percent_Identity=64.741641337386, Blast_Score=469, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI32566331, Length=310, Percent_Identity=38.7096774193548, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI32566329, Length=330, Percent_Identity=38.1818181818182, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17561210, Length=339, Percent_Identity=37.1681415929204, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI28574488, Length=314, Percent_Identity=35.031847133758, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574490, Length=314, Percent_Identity=35.031847133758, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_VIBSL (B7VLU4)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416600.1
- ProteinModelPortal:   B7VLU4
- GeneID:   7160577
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0985
- HOGENOM:   HBG529885
- OMA:   KHHVSGV
- ProtClustDB:   PRK00164
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 37802; Mature: 37802

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 22-22 BINDING 35-35 BINDING 73-73 BINDING 77-77 BINDING 104-104 BINDING 128-128 BINDING 165-165 BINDING 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERCSVAQQFEDRFHRKFYYLRLSVTDVCNFKCTYCLPDGYKPSGQKNSSFLSVPEIKRV
CCCCHHHHHHHHHHHHEEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCEECCHHHHHH
VKAFADCGTSKIRITGGEPSLRKDFPEIIHTVASTPGIEKVATTTNGYRMEKQVGQWRDA
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHEECCCCEEEHHHHHHHHHC
GLTHINVSVDSLDSRMFHQITGENKFTEVMRGIDKAFEVGFEQVKVNVVLMKDLNSQELP
CCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHEEEEEEEEECCCCCCHH
AFLNWIKDKPIQLRFIELMKTGEMDELFDKHHVSGVAIRNQLIANGWLLKVKAVNDGPAQ
HHHHHHCCCCCEEEEEHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCEEEEEEEECCCCEE
VFVHPDYQGEIGLIMPYEKDFCESCNRLRVSATGKLHLCLFGDHGVELRDLIQEDQQEQE
EEECCCCCCCEEEEECCCHHHHHHHCCEEEEECCEEEEEEECCCCCHHHHHHHHCHHHHH
LIDRIQAQLQTKSVSHFLHDGNSGMTPNLASIGG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCC
>Mature Secondary Structure
MERCSVAQQFEDRFHRKFYYLRLSVTDVCNFKCTYCLPDGYKPSGQKNSSFLSVPEIKRV
CCCCHHHHHHHHHHHHEEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCEECCHHHHHH
VKAFADCGTSKIRITGGEPSLRKDFPEIIHTVASTPGIEKVATTTNGYRMEKQVGQWRDA
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHEECCCCEEEHHHHHHHHHC
GLTHINVSVDSLDSRMFHQITGENKFTEVMRGIDKAFEVGFEQVKVNVVLMKDLNSQELP
CCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHEEEEEEEEECCCCCCHH
AFLNWIKDKPIQLRFIELMKTGEMDELFDKHHVSGVAIRNQLIANGWLLKVKAVNDGPAQ
HHHHHHCCCCCEEEEEHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCEEEEEEEECCCCEE
VFVHPDYQGEIGLIMPYEKDFCESCNRLRVSATGKLHLCLFGDHGVELRDLIQEDQQEQE
EEECCCCCCCEEEEECCCHHHHHHHCCEEEEECCEEEEEEECCCCCHHHHHHHHCHHHHH
LIDRIQAQLQTKSVSHFLHDGNSGMTPNLASIGG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA