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The map label for this gene is purF [H]

Identifier: 218708907

GI number: 218708907

Start: 936276

End: 937790

Strand: Direct

Name: purF [H]

Synonym: VS_0908

Alternate gene names: 218708907

Gene position: 936276-937790 (Clockwise)

Preceding gene: 218708906

Following gene: 218708909

Centisome position: 28.38

GC content: 44.88

Gene sequence:

>1515_bases
ATGTGTGGTATTGTTGGAATCGTGGGTTCAACACCTGTAAACCAGTCTATTTATGACGCTTTAACGGTATTGCAGCATCG
TGGCCAAGATGCCGCTGGTATTTGTACCATAGAAAGCAATCGTTTCCGTCTGCGCAAGGCGAACGGTTTAGTTAAAGATG
TTTTTGAAGCAAAACACATGCAACGCCTCCAAGGCAACGTGGGTATTGGTCATGTTCGTTATCCTACAGCGGGAAGTTCG
AGTGCTTCTGAAGCTCAACCTTTCTACGTAAACTCTCCATTTGGCATCACGCTGGCGCACAACGGTAACTTAACGAATGC
AAGTGAAGTTCGTGAGAAGTTGTTCGAAAAAGACCGCCGTCATGTCAACACAACCTCTGACTCTGAAGTTCTACTAAACG
TATTGGCTCATGAAATCGATACCGTAAAAGGTAACGTGACTTCAGATGACGTTTTCCGCGCAGTTGCAAACGTGCACCGC
ACTATTCGTGGTGCTTACGCAGTAACAGCGATGATCATCGGCCACGGCATGATCGCATTCCGTGACCCACATGGTATTCG
TCCACTGTGTCTTGGTAAGCGTGAAATTAACGGTAAAACAGAGTACATGGTTGCGTCTGAGTCAGTAGCGTTAGACGCTG
TTGGTTTTGATTTTATGCGCGATGTTGCTCCTGGTGAAGCTATCTATGCAACATTCGATGGTGAGCTTTTCACTAAACAA
TGCGCAGACAACCCACAACTAAACCCATGTATTTTTGAGTTTGTTTATTTTGCTCGTCCTGATTCATTCATCGATAAAAT
TTCGGTTTACAGCGCACGCGTTGAGATGGGTGAGATGTTAGGTAAGCGTATTAAAGAAGAGTACGCAGACCTAGACATTG
ACGTGGTTATTCCAATTCCTGAAACATCAAATGATATTGCTCTACGAATTGCTCAAGCGATTGATAAGCCATATCGTCAA
GGTTTCGTGAAAAACCGTTACGTTGGCCGCACGTTTATCATGCCTGGTCAGCAACAACGTAAGAAGTCGGTTCGCCGTAA
ACTCAACGCGATTCGTTCTGAGTTTAAAGGTAAGAACGTTCTACTGGTTGATGACTCGATTGTTCGTGGTACAACATCAG
AGCAGATCATTGAGATGGCTCGTGATTCTGGTGCAAACAAAGTCTTCATGGTTTCTGCCGCTCCGGAGGTTCGCTTCCCG
AACGTTTACGGCATTGATATGCCGAGCGCGACTGAGTTAATTGCTCATGGTCGTGATAACGAAACGATTTGTAAGCAGAT
TGGTGCAGATGCTTTGATTTTCCAAACCTTACCAGACTTAATCTCTGCAGTAGGTATGGGTAATCAAGACATCTCTCGTT
TTGATACATCCGTATTCAACGGTGAGTATGTAACGGGCGATATCGACCAAGCGTACCTAGATTTCCTAGATTCTTTACGT
AATGACGATTCAAAGGTTGAGCGTGAGATCCAACAAGATCTCGCGAACCTAGAGTTACATAACGAAGGCGCTTAG

Upstream 100 bases:

>100_bases
CCGAATCATTGCGCCGTTCTTCGAGCATTTACAAGCAACATCTAGTTTCTTATCTGGCGCGCTTTAGCGCCAGTTAATGT
CGAAAATCGAGGATTAGGAC

Downstream 100 bases:

>100_bases
CGTACTCATAAACATGAGTAATAAAAAACCAGAGCATCGACTCTGGTTTTTTTGTTTGCGGGGAAGGGGATTGAATCTTA
GTGAATGTGGTATGCGATCA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MCGIVGIVGSTPVNQSIYDALTVLQHRGQDAAGICTIESNRFRLRKANGLVKDVFEAKHMQRLQGNVGIGHVRYPTAGSS
SASEAQPFYVNSPFGITLAHNGNLTNASEVREKLFEKDRRHVNTTSDSEVLLNVLAHEIDTVKGNVTSDDVFRAVANVHR
TIRGAYAVTAMIIGHGMIAFRDPHGIRPLCLGKREINGKTEYMVASESVALDAVGFDFMRDVAPGEAIYATFDGELFTKQ
CADNPQLNPCIFEFVYFARPDSFIDKISVYSARVEMGEMLGKRIKEEYADLDIDVVIPIPETSNDIALRIAQAIDKPYRQ
GFVKNRYVGRTFIMPGQQQRKKSVRRKLNAIRSEFKGKNVLLVDDSIVRGTTSEQIIEMARDSGANKVFMVSAAPEVRFP
NVYGIDMPSATELIAHGRDNETICKQIGADALIFQTLPDLISAVGMGNQDISRFDTSVFNGEYVTGDIDQAYLDFLDSLR
NDDSKVEREIQQDLANLELHNEGA

Sequences:

>Translated_504_residues
MCGIVGIVGSTPVNQSIYDALTVLQHRGQDAAGICTIESNRFRLRKANGLVKDVFEAKHMQRLQGNVGIGHVRYPTAGSS
SASEAQPFYVNSPFGITLAHNGNLTNASEVREKLFEKDRRHVNTTSDSEVLLNVLAHEIDTVKGNVTSDDVFRAVANVHR
TIRGAYAVTAMIIGHGMIAFRDPHGIRPLCLGKREINGKTEYMVASESVALDAVGFDFMRDVAPGEAIYATFDGELFTKQ
CADNPQLNPCIFEFVYFARPDSFIDKISVYSARVEMGEMLGKRIKEEYADLDIDVVIPIPETSNDIALRIAQAIDKPYRQ
GFVKNRYVGRTFIMPGQQQRKKSVRRKLNAIRSEFKGKNVLLVDDSIVRGTTSEQIIEMARDSGANKVFMVSAAPEVRFP
NVYGIDMPSATELIAHGRDNETICKQIGADALIFQTLPDLISAVGMGNQDISRFDTSVFNGEYVTGDIDQAYLDFLDSLR
NDDSKVEREIQQDLANLELHNEGA
>Mature_504_residues
MCGIVGIVGSTPVNQSIYDALTVLQHRGQDAAGICTIESNRFRLRKANGLVKDVFEAKHMQRLQGNVGIGHVRYPTAGSS
SASEAQPFYVNSPFGITLAHNGNLTNASEVREKLFEKDRRHVNTTSDSEVLLNVLAHEIDTVKGNVTSDDVFRAVANVHR
TIRGAYAVTAMIIGHGMIAFRDPHGIRPLCLGKREINGKTEYMVASESVALDAVGFDFMRDVAPGEAIYATFDGELFTKQ
CADNPQLNPCIFEFVYFARPDSFIDKISVYSARVEMGEMLGKRIKEEYADLDIDVVIPIPETSNDIALRIAQAIDKPYRQ
GFVKNRYVGRTFIMPGQQQRKKSVRRKLNAIRSEFKGKNVLLVDDSIVRGTTSEQIIEMARDSGANKVFMVSAAPEVRFP
NVYGIDMPSATELIAHGRDNETICKQIGADALIFQTLPDLISAVGMGNQDISRFDTSVFNGEYVTGDIDQAYLDFLDSLR
NDDSKVEREIQQDLANLELHNEGA

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=476, Percent_Identity=35.5042016806723, Blast_Score=272, Evalue=6e-73,
Organism=Escherichia coli, GI1788651, Length=507, Percent_Identity=75.5424063116371, Blast_Score=781, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=223, Percent_Identity=30.9417040358744, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=483, Percent_Identity=33.5403726708075, Blast_Score=240, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6323958, Length=509, Percent_Identity=49.3123772102161, Blast_Score=475, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24659598, Length=493, Percent_Identity=34.0770791075051, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24659604, Length=469, Percent_Identity=34.5415778251599, Blast_Score=265, Evalue=8e-71,
Organism=Drosophila melanogaster, GI28573187, Length=501, Percent_Identity=32.9341317365269, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55783; Mature: 55783

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVGSTPVNQSIYDALTVLQHRGQDAAGICTIESNRFRLRKANGLVKDVFEAKHM
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHHCCHHHHHHHHHHH
QRLQGNVGIGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNASEVREKLFEKDRR
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HVNTTSDSEVLLNVLAHEIDTVKGNVTSDDVFRAVANVHRTIRGAYAVTAMIIGHGMIAF
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
RDPHGIRPLCLGKREINGKTEYMVASESVALDAVGFDFMRDVAPGEAIYATFDGELFTKQ
ECCCCCCCCCCCCHHCCCCEEEEEECCCEEEHHHHHHHHHHCCCCCEEEEEECCHHHHHH
CADNPQLNPCIFEFVYFARPDSFIDKISVYSARVEMGEMLGKRIKEEYADLDIDVVIPIP
HCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
ETSNDIALRIAQAIDKPYRQGFVKNRYVGRTFIMPGQQQRKKSVRRKLNAIRSEFKGKNV
CCCCHHHHHHHHHHCHHHHHCHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCEE
LLVDDSIVRGTTSEQIIEMARDSGANKVFMVSAAPEVRFPNVYGIDMPSATELIAHGRDN
EEECCCHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCHHHHHHCCCCC
ETICKQIGADALIFQTLPDLISAVGMGNQDISRFDTSVFNGEYVTGDIDQAYLDFLDSLR
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHC
NDDSKVEREIQQDLANLELHNEGA
CCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MCGIVGIVGSTPVNQSIYDALTVLQHRGQDAAGICTIESNRFRLRKANGLVKDVFEAKHM
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHHCCHHHHHHHHHHH
QRLQGNVGIGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNASEVREKLFEKDRR
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HVNTTSDSEVLLNVLAHEIDTVKGNVTSDDVFRAVANVHRTIRGAYAVTAMIIGHGMIAF
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
RDPHGIRPLCLGKREINGKTEYMVASESVALDAVGFDFMRDVAPGEAIYATFDGELFTKQ
ECCCCCCCCCCCCHHCCCCEEEEEECCCEEEHHHHHHHHHHCCCCCEEEEEECCHHHHHH
CADNPQLNPCIFEFVYFARPDSFIDKISVYSARVEMGEMLGKRIKEEYADLDIDVVIPIP
HCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
ETSNDIALRIAQAIDKPYRQGFVKNRYVGRTFIMPGQQQRKKSVRRKLNAIRSEFKGKNV
CCCCHHHHHHHHHHCHHHHHCHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCEE
LLVDDSIVRGTTSEQIIEMARDSGANKVFMVSAAPEVRFPNVYGIDMPSATELIAHGRDN
EEECCCHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCHHHHHHCCCCC
ETICKQIGADALIFQTLPDLISAVGMGNQDISRFDTSVFNGEYVTGDIDQAYLDFLDSLR
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHC
NDDSKVEREIQQDLANLELHNEGA
CCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]