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The map label for this gene is truA [H]

Identifier: 218708902

GI number: 218708902

Start: 931912

End: 932706

Strand: Direct

Name: truA [H]

Synonym: VS_0903

Alternate gene names: 218708902

Gene position: 931912-932706 (Clockwise)

Preceding gene: 218708901

Following gene: 218708903

Centisome position: 28.25

GC content: 44.03

Gene sequence:

>795_bases
ATGAAAATTGCTTTAGGTATTGAATATAACGGTACCCACTACTTTGGTTGGCAGCGCCAACGAGACGTGAAAAGTGTCCA
AGAAGAATTGGAAAAGGCTCTTTCAGTTGTCGCGAATCACCCTGTAGAAGTTATGTGTGCTGGTCGCACAGATGCCGGTG
TTCACGGTACGGGACAAGTCGTTCACTTTGAAACTAATGTCGATCGTAAAATGGTTGCGTGGACAATGGGCGCAAATGCC
AACATGCCAAAAGATATTGCCGTTCGTTGGGCAACAGAAGTGAATGAGGATTTCCATGCTCGTTTTTCTGCAACAGCACG
CCGTTATCGCTACATTATCTTTAACCATGCTCTACGTCCTGGCATTTTGAATTCAGGTGTGAGCCATTACCATGGTCACC
TTGATGAGAAAAAGATGCATGAAGCGGGTCAGTACTTACTTGGTGAGAATGACTTTACTTCGTTCAGGGCAACACATTGT
CAATCTCGTAGCCCATGGAGAAACATGATTCACTTAAACGTGACTCGTCATGGGCATTACATAGTGATCGATATTAAAGC
GAATGCGTTTGTTCACCATATGGTGAGGAATATTACTGGTAGCCTAATTGCGGTAGGTAAAGGTGATCAAAAACCAGAGT
GGATTCAGTGGCTTTTAGAGGCTAAAGATCGAAAAGTGGCAGGCGCGACAGCGAAAGCGGAAGGCTTATACTTAGTAGAT
GTTGATTATCCTGAACATTTTGGACTACCAAGAGAGCCTATCGGCCCTCTATTTTTGCCGGATAATTTGAACTAA

Upstream 100 bases:

>100_bases
AGGCGGTAGTGATTGTTTATTGAAGTTTTAGCCCCATCCTATTTGGGCTAGCACTCATTTCCGTATATACTTCTCGCCCA
TTTTCAAAGAGAACAAGAAC

Downstream 100 bases:

>100_bases
ATGTGAGACTTAGGTTCAAAAAATATTACGGTTTCGTGCGTAAAATAGTCACGGAAAATAGGTACAACCAGTTTTTTATC
TACAGTCGCTGTTTTATGTG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKIALGIEYNGTHYFGWQRQRDVKSVQEELEKALSVVANHPVEVMCAGRTDAGVHGTGQVVHFETNVDRKMVAWTMGANA
NMPKDIAVRWATEVNEDFHARFSATARRYRYIIFNHALRPGILNSGVSHYHGHLDEKKMHEAGQYLLGENDFTSFRATHC
QSRSPWRNMIHLNVTRHGHYIVIDIKANAFVHHMVRNITGSLIAVGKGDQKPEWIQWLLEAKDRKVAGATAKAEGLYLVD
VDYPEHFGLPREPIGPLFLPDNLN

Sequences:

>Translated_264_residues
MKIALGIEYNGTHYFGWQRQRDVKSVQEELEKALSVVANHPVEVMCAGRTDAGVHGTGQVVHFETNVDRKMVAWTMGANA
NMPKDIAVRWATEVNEDFHARFSATARRYRYIIFNHALRPGILNSGVSHYHGHLDEKKMHEAGQYLLGENDFTSFRATHC
QSRSPWRNMIHLNVTRHGHYIVIDIKANAFVHHMVRNITGSLIAVGKGDQKPEWIQWLLEAKDRKVAGATAKAEGLYLVD
VDYPEHFGLPREPIGPLFLPDNLN
>Mature_264_residues
MKIALGIEYNGTHYFGWQRQRDVKSVQEELEKALSVVANHPVEVMCAGRTDAGVHGTGQVVHFETNVDRKMVAWTMGANA
NMPKDIAVRWATEVNEDFHARFSATARRYRYIIFNHALRPGILNSGVSHYHGHLDEKKMHEAGQYLLGENDFTSFRATHC
QSRSPWRNMIHLNVTRHGHYIVIDIKANAFVHHMVRNITGSLIAVGKGDQKPEWIQWLLEAKDRKVAGATAKAEGLYLVD
VDYPEHFGLPREPIGPLFLPDNLN

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=267, Percent_Identity=29.2134831460674, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1788657, Length=260, Percent_Identity=70.3846153846154, Blast_Score=396, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29957; Mature: 29957

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALGIEYNGTHYFGWQRQRDVKSVQEELEKALSVVANHPVEVMCAGRTDAGVHGTGQV
CEEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEE
VHFETNVDRKMVAWTMGANANMPKDIAVRWATEVNEDFHARFSATARRYRYIIFNHALRP
EEEECCCCCEEEEEEECCCCCCCHHHEEEEEHHCCHHHHHHHHHHHHHEEEEEEECCCCC
GILNSGVSHYHGHLDEKKMHEAGQYLLGENDFTSFRATHCQSRSPWRNMIHLNVTRHGHY
CHHHCCHHHHCCCCCHHHHHHHCCEEECCCCCCHHHHHHCCCCCCCCCEEEEEEEECCCE
IVIDIKANAFVHHMVRNITGSLIAVGKGDQKPEWIQWLLEAKDRKVAGATAKAEGLYLVD
EEEEEECCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCCCEEEEE
VDYPEHFGLPREPIGPLFLPDNLN
ECCHHHCCCCCCCCCCEECCCCCC
>Mature Secondary Structure
MKIALGIEYNGTHYFGWQRQRDVKSVQEELEKALSVVANHPVEVMCAGRTDAGVHGTGQV
CEEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEE
VHFETNVDRKMVAWTMGANANMPKDIAVRWATEVNEDFHARFSATARRYRYIIFNHALRP
EEEECCCCCEEEEEEECCCCCCCHHHEEEEEHHCCHHHHHHHHHHHHHEEEEEEECCCCC
GILNSGVSHYHGHLDEKKMHEAGQYLLGENDFTSFRATHCQSRSPWRNMIHLNVTRHGHY
CHHHCCHHHHCCCCCHHHHHHHCCEEECCCCCCHHHHHHCCCCCCCCCEEEEEEEECCCE
IVIDIKANAFVHHMVRNITGSLIAVGKGDQKPEWIQWLLEAKDRKVAGATAKAEGLYLVD
EEEEEECCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCCCEEEEE
VDYPEHFGLPREPIGPLFLPDNLN
ECCHHHCCCCCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA