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The map label for this gene is folD [H]

Identifier: 218708889

GI number: 218708889

Start: 914381

End: 915238

Strand: Reverse

Name: folD [H]

Synonym: VS_0889

Alternate gene names: 218708889

Gene position: 915238-914381 (Counterclockwise)

Preceding gene: 218708890

Following gene: 218708883

Centisome position: 27.74

GC content: 47.67

Gene sequence:

>858_bases
ATGACTGCTCAAAATATTGATGGAAAGCTAATTTCTCAAACGGTTCGCTCTGAAGTTGCGGCTCGTGTAAAAGCTCGCAC
TCAAGCTGGATTACGCGCTCCGGGCCTAGCGGTTGTTTTAGTGGGTGAAGACCCTGCTTCTCAGGTTTACGTAGGAAGTA
AACGTAAAGCATGTGAAGAAGTTGGCTTCGTATCTAAATCTTACGACTTGCCAGCCACTGCGACAGAAGACGAATTATTA
ACACTGGTAGACCAACTGAACGAAGACCCGGAGATTGACGGTATTCTTGTTCAACTTCCTCTACCAGCTGGTATTGATAG
CACTCAAGTTCTTGAGCGCATAACGCCGGAAAAAGACGTTGATGGCTTCCACCCATACAACGTAGGTCGTTTGGCTCAGC
GTATGCCTAAGCTTCGCTCTTGTACGCCTAAAGGCATCATTACGCTGCTTGACCGTTATAACATCGACTTACGTGGCAAA
CACGCGGTTGTGGTTGGCGCATCAAACATCGTAGGTCGCCCAATGACGTTAGAGCTTCTTCTAGCCGGTTGTACAACGAC
AACATGTCACCGCTTCACCAAAGACCTTGAGGGTCATGTACGTCAAGCAGATGTTGTTGTTGTAGCCGTTGGTAAGCCTA
ACTTCATTCCTGGTGCTTGGATTAAGAAAGGTGCTGTTGTGGTCGATGTAGGCATCAATCGTTTGGAATCTGGCAAACTC
GTTGGCGACGTTGAATACGATGTCGCGAAAGAGAGCGCAAGCTTCATCACACCAGTTCCGGGTGGTGTTGGTCCAATGAC
AGTAGCAAGCCTAATCGAGAACACCATGATCGCTTGTGAGCAATTCCACTCTAAATAA

Upstream 100 bases:

>100_bases
AACAAAGCAAACGTTTGCTTGATGGTAATCAAAAAGATTTTCTGGAATAATGCGCCAAAAACATCAGCCACTAACTTGAA
TATCAATACTAAGGAAATTC

Downstream 100 bases:

>100_bases
TCTGCGAACGACAGCATAAACAGAATTAAAAAGCCGCAGCGTTAAAACGTTGCGGCTTTTTTGATACTGAGCTATTCGTT
TTTGATACAAAGTGACTCGC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTAQNIDGKLISQTVRSEVAARVKARTQAGLRAPGLAVVLVGEDPASQVYVGSKRKACEEVGFVSKSYDLPATATEDELL
TLVDQLNEDPEIDGILVQLPLPAGIDSTQVLERITPEKDVDGFHPYNVGRLAQRMPKLRSCTPKGIITLLDRYNIDLRGK
HAVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKDLEGHVRQADVVVVAVGKPNFIPGAWIKKGAVVVDVGINRLESGKL
VGDVEYDVAKESASFITPVPGGVGPMTVASLIENTMIACEQFHSK

Sequences:

>Translated_285_residues
MTAQNIDGKLISQTVRSEVAARVKARTQAGLRAPGLAVVLVGEDPASQVYVGSKRKACEEVGFVSKSYDLPATATEDELL
TLVDQLNEDPEIDGILVQLPLPAGIDSTQVLERITPEKDVDGFHPYNVGRLAQRMPKLRSCTPKGIITLLDRYNIDLRGK
HAVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKDLEGHVRQADVVVVAVGKPNFIPGAWIKKGAVVVDVGINRLESGKL
VGDVEYDVAKESASFITPVPGGVGPMTVASLIENTMIACEQFHSK
>Mature_284_residues
TAQNIDGKLISQTVRSEVAARVKARTQAGLRAPGLAVVLVGEDPASQVYVGSKRKACEEVGFVSKSYDLPATATEDELLT
LVDQLNEDPEIDGILVQLPLPAGIDSTQVLERITPEKDVDGFHPYNVGRLAQRMPKLRSCTPKGIITLLDRYNIDLRGKH
AVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKDLEGHVRQADVVVVAVGKPNFIPGAWIKKGAVVVDVGINRLESGKLV
GDVEYDVAKESASFITPVPGGVGPMTVASLIENTMIACEQFHSK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=294, Percent_Identity=43.8775510204082, Blast_Score=226, Evalue=1e-59,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=41.0958904109589, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI94721354, Length=291, Percent_Identity=42.9553264604811, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI36796743, Length=209, Percent_Identity=30.1435406698565, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=73.1448763250883, Blast_Score=434, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17568735, Length=287, Percent_Identity=41.8118466898955, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=45.9731543624161, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=43.205574912892, Blast_Score=223, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322933, Length=312, Percent_Identity=27.5641025641026, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17136816, Length=299, Percent_Identity=47.1571906354515, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI17136818, Length=299, Percent_Identity=47.1571906354515, Blast_Score=251, Evalue=5e-67,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=43.1034482758621, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=43.1034482758621, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=43.1034482758621, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=43.1034482758621, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30549; Mature: 30417

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQNIDGKLISQTVRSEVAARVKARTQAGLRAPGLAVVLVGEDPASQVYVGSKRKACEE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFVSKSYDLPATATEDELLTLVDQLNEDPEIDGILVQLPLPAGIDSTQVLERITPEKDV
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLAQRMPKLRSCTPKGIITLLDRYNIDLRGKHAVVVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKDLEGHVRQADVVVVAVGKPNFIPGAWIKKGAVVVDVGINRLESGKL
HHCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHCCCEEEEECCCCCCCCCE
VGDVEYDVAKESASFITPVPGGVGPMTVASLIENTMIACEQFHSK
EECCHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAQNIDGKLISQTVRSEVAARVKARTQAGLRAPGLAVVLVGEDPASQVYVGSKRKACEE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFVSKSYDLPATATEDELLTLVDQLNEDPEIDGILVQLPLPAGIDSTQVLERITPEKDV
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLAQRMPKLRSCTPKGIITLLDRYNIDLRGKHAVVVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKDLEGHVRQADVVVVAVGKPNFIPGAWIKKGAVVVDVGINRLESGKL
HHCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHCCCEEEEECCCCCCCCCE
VGDVEYDVAKESASFITPVPGGVGPMTVASLIENTMIACEQFHSK
EECCHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA