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The map label for this gene is rhtB [H]

Identifier: 218708870

GI number: 218708870

Start: 894015

End: 894656

Strand: Direct

Name: rhtB [H]

Synonym: VS_0870

Alternate gene names: 218708870

Gene position: 894015-894656 (Clockwise)

Preceding gene: 218708869

Following gene: 218708871

Centisome position: 27.1

GC content: 43.61

Gene sequence:

>642_bases
ATGACCTTTGATACATGGATTTATTATTTGCTAGCGGTGTTGATCTTAACGGCATCACCAGGGCCAAGCTCTTTGTTGTG
CATGACTAAGGGTGTGCAATCTGGCTTTAAGTTATCGATATTTACCGCCTTGGGTAGCGTGACTGCCATTACCGGAATCC
TTACGTTATCTTTTACCGGTTTAGGGGTGATCATTGCTTCGTCAGAAGTGGTGTTTAATATCATTAAGTGGACGGGGGCT
GCTTACCTTATTTATCTCGGTTGGAAGTCGTTACGATCGAGTCAGCAAGATTACGACAAGTTATCGAATCAACAAGCAGG
CTCTCAACCTGTTAAAGAAAGCGCTGCACAGCATTATGTCAGTGGCTTTATTGTTGGTGCGAGTAACCCAAAAGCTATCC
TGTTTTTTACCGCACTTTTTCCTCAGTTTATTGACCCATCGATAGCGCTATTTCCTCAATTTTTGGTGTTCGCTTTGACC
TTCGTTGTGATGGAATTGTCTTGGTTACTGGTTTATGCCTACCTAGGTGCGAAATCATCAAATTGGTTGTTTGCTAAAGG
TAGAGCCAAGGTTTTTAATCTTGTGACTGGTGGTGTCTTTATCGGTGCAGGGGCGCTGCTTTCTACTACAAGCCGAGCGT
AA

Upstream 100 bases:

>100_bases
ACAGAGTGAACGTGCTCAATATTTAGTTGCTTAATATGAATAGCTATCACATATTGGTGCTCAATATTTAGTCGTTTTAG
TGAGTTAAGGAGAATTCGCG

Downstream 100 bases:

>100_bases
TTTTCAATTTATCAAAGGCCTGAATAGTTACAGAAAATTATTCAGGCTGTAATAAAAGGTTAATATTAATGTGCTTGCTG
ATATCGGCATAAAGTCGATA

Product: LysE family transporter

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTFDTWIYYLLAVLILTASPGPSSLLCMTKGVQSGFKLSIFTALGSVTAITGILTLSFTGLGVIIASSEVVFNIIKWTGA
AYLIYLGWKSLRSSQQDYDKLSNQQAGSQPVKESAAQHYVSGFIVGASNPKAILFFTALFPQFIDPSIALFPQFLVFALT
FVVMELSWLLVYAYLGAKSSNWLFAKGRAKVFNLVTGGVFIGAGALLSTTSRA

Sequences:

>Translated_213_residues
MTFDTWIYYLLAVLILTASPGPSSLLCMTKGVQSGFKLSIFTALGSVTAITGILTLSFTGLGVIIASSEVVFNIIKWTGA
AYLIYLGWKSLRSSQQDYDKLSNQQAGSQPVKESAAQHYVSGFIVGASNPKAILFFTALFPQFIDPSIALFPQFLVFALT
FVVMELSWLLVYAYLGAKSSNWLFAKGRAKVFNLVTGGVFIGAGALLSTTSRA
>Mature_212_residues
TFDTWIYYLLAVLILTASPGPSSLLCMTKGVQSGFKLSIFTALGSVTAITGILTLSFTGLGVIIASSEVVFNIIKWTGAA
YLIYLGWKSLRSSQQDYDKLSNQQAGSQPVKESAAQHYVSGFIVGASNPKAILFFTALFPQFIDPSIALFPQFLVFALTF
VVMELSWLLVYAYLGAKSSNWLFAKGRAKVFNLVTGGVFIGAGALLSTTSRA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=214, Percent_Identity=31.7757009345794, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788099, Length=205, Percent_Identity=30.2439024390244, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22954; Mature: 22823

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFDTWIYYLLAVLILTASPGPSSLLCMTKGVQSGFKLSIFTALGSVTAITGILTLSFTG
CCHHHHHHHHHHHHHHCCCCCCCCEEEEHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCC
LGVIIASSEVVFNIIKWTGAAYLIYLGWKSLRSSQQDYDKLSNQQAGSQPVKESAAQHYV
CEEEEECHHHHHHHHHHCCCEEEEEECHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHH
SGFIVGASNPKAILFFTALFPQFIDPSIALFPQFLVFALTFVVMELSWLLVYAYLGAKSS
HEEEEECCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NWLFAKGRAKVFNLVTGGVFIGAGALLSTTSRA
CEEEECCCHHHHHHHHCHHHHHCCHHHHCCCCC
>Mature Secondary Structure 
TFDTWIYYLLAVLILTASPGPSSLLCMTKGVQSGFKLSIFTALGSVTAITGILTLSFTG
CHHHHHHHHHHHHHHCCCCCCCCEEEEHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCC
LGVIIASSEVVFNIIKWTGAAYLIYLGWKSLRSSQQDYDKLSNQQAGSQPVKESAAQHYV
CEEEEECHHHHHHHHHHCCCEEEEEECHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHH
SGFIVGASNPKAILFFTALFPQFIDPSIALFPQFLVFALTFVVMELSWLLVYAYLGAKSS
HEEEEECCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NWLFAKGRAKVFNLVTGGVFIGAGALLSTTSRA
CEEEECCCHHHHHHHHCHHHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]