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The map label for this gene is hlyU [H]

Identifier: 218708566

GI number: 218708566

Start: 552087

End: 552380

Strand: Direct

Name: hlyU [H]

Synonym: VS_0532

Alternate gene names: 218708566

Gene position: 552087-552380 (Clockwise)

Preceding gene: 218708565

Following gene: 218708568

Centisome position: 16.73

GC content: 43.2

Gene sequence:

>294_bases
ATGAACTTAAAAGAGATGGAGAAGAATTCAGCACAAGCTGTGATTCTACTCAAAGCCATGGCCAATGAGCGTCGCTTACA
GATTCTGTGCCTATTGCATGGTACTGAGCTGTCGGTTGGGGAGTTGTGTGGCAAGTTGGAATTGAGTCAATCTGCTTTAT
CTCAACATCTTGCTTGGTTGAGAAGAGATGGTTTGGTCGAAACTCGCAAAGAAGCTCAAACAGTGTATTACACATTGAGC
AGTGAAGAAGTAAAAGCGATGATTAACCTGCTGCATGGTATGTACTGCAAGTAG

Upstream 100 bases:

>100_bases
GCTTGTTTGTTAATGAATTGATATCGATTATCATGACTTCATAATCTGCCGGAAATACTGATAACCCGGACTTGGCGTAA
AGGTTGTATGGAGCCAAACT

Downstream 100 bases:

>100_bases
TTTATTGCTGGTGGGGGAAGTGATTTCTTACCAGGGGTAGGGTCGACACAAGGAAGTGTCAGTATTTCATGTCGAACGTT
AGTGCCTATCAGTAAACCGC

Product: transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 97; Mature: 97

Protein sequence:

>97_residues
MNLKEMEKNSAQAVILLKAMANERRLQILCLLHGTELSVGELCGKLELSQSALSQHLAWLRRDGLVETRKEAQTVYYTLS
SEEVKAMINLLHGMYCK

Sequences:

>Translated_97_residues
MNLKEMEKNSAQAVILLKAMANERRLQILCLLHGTELSVGELCGKLELSQSALSQHLAWLRRDGLVETRKEAQTVYYTLS
SEEVKAMINLLHGMYCK
>Mature_97_residues
MNLKEMEKNSAQAVILLKAMANERRLQILCLLHGTELSVGELCGKLELSQSALSQHLAWLRRDGLVETRKEAQTVYYTLS
SEEVKAMINLLHGMYCK

Specific function: Up-regulates the expression of the hemolysin gene, hlyA, and may promote expression of other virulence determinants in vivo. It may have both positive and negative regulator activities [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=96, Percent_Identity=42.7083333333333, Blast_Score=81, Evalue=1e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 10992; Mature: 10992

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKEMEKNSAQAVILLKAMANERRLQILCLLHGTELSVGELCGKLELSQSALSQHLAWL
CCCHHHCCCCCHHEEHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RRDGLVETRKEAQTVYYTLSSEEVKAMINLLHGMYCK
HHCCCHHHHHCCEEEEEEECHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLKEMEKNSAQAVILLKAMANERRLQILCLLHGTELSVGELCGKLELSQSALSQHLAWL
CCCHHHCCCCCHHEEHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RRDGLVETRKEAQTVYYTLSSEEVKAMINLLHGMYCK
HHCCCHHHHHCCEEEEEEECHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8231807; 9457888; 10952301 [H]