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The map label for this gene is nfo [H]

Identifier: 218708534

GI number: 218708534

Start: 518876

End: 519784

Strand: Direct

Name: nfo [H]

Synonym: VS_0499

Alternate gene names: 218708534

Gene position: 518876-519784 (Clockwise)

Preceding gene: 218708532

Following gene: 218708535

Centisome position: 15.73

GC content: 44.44

Gene sequence:

>909_bases
ATGACAAACAATAATTCATCTTCAAAACCTGAAACTAATATTAAGTTTATTGGTGCGCATGTGTCTGCTGCGGGCGGTGT
TGATCAGGCGCCAATGCGCGCTCGTGAGATTGGCGCCAATGCTTTTGCACTGTTCACCAAAAACCAAAGACAGTGGGCAG
CAAAGCCGCTAGAAGCCAAAACCATCAGCGCCTTTAAAGCCAACTGTAAAATGTTAGGTTTTAGCGCTGAGCACATCCTT
CCTCATGACTCCTACCTTATTAATTTAGGAGCGCCAGAAGAAGAGAAGTTAGAGAAATCGCGTGCCGCTTTTATTGATGA
AATGGAGCGCTGCAATCAACTTGGATTGACGCTTTTGAATTTTCATCCTGGGAGTCATTTAAAGAAAATCTCAGAAAGCG
AATGTTTGGCCAAGATCGCCGAGTCTATCAATTTGGCTCATCAAGCGGTGCCTGATGTGATTGCGGTGATTGAGAATACC
TCAGGGCAGGGGACTAACCTTGGCTGGAAGTTTGAGCAACTAGCAGAGATCATCGATCAAGTAGAAGATAAATCACGCGT
CGGTGTGTGTTTAGACACTTGCCATACTTTTACAGCCGGTTATGACCTACGAACAAAAGAAGCATGTGATCATACTTTTG
CTGAGTTCGACCGCATTGTCGGCATGCACTATCTAAGAGCAATGCACATCAATGATTCAAAAGCTGAGTTCGCGAGTCGA
GTCGATCGACATCATTCTTTAGGAAAAGGCGAGATTGGCTGGGATTGTTTTGAGTATATTGCCTCGGATTCTCGCTTTAA
TGGTATTCCTCTTATCTTGGAAACCATTGATTCGACGATCTGGAAAGAGGAAATTCAACAACTTAGAATGTTCCATCGCT
CAGCAACGGCAGTGAGCGAAGAAGCGTAA

Upstream 100 bases:

>100_bases
GGCATTTGTTGTAAATTTACTACATTTGTTGTGTTTTTATTGACCTGTAAGTGATACGACCCTTTAATTATTAAGCATCC
TGAAAGTGTAAAGTGATTTA

Downstream 100 bases:

>100_bases
TAATAGAGGGGGAAATTTATTTCCGTCTATTTCTAATAATTGGCACCTTTCTTTCATAGTATTAAAGTGAATATGTAGTT
CACGATGTCTTAGAGGAGGT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTNNNSSSKPETNIKFIGAHVSAAGGVDQAPMRAREIGANAFALFTKNQRQWAAKPLEAKTISAFKANCKMLGFSAEHIL
PHDSYLINLGAPEEEKLEKSRAAFIDEMERCNQLGLTLLNFHPGSHLKKISESECLAKIAESINLAHQAVPDVIAVIENT
SGQGTNLGWKFEQLAEIIDQVEDKSRVGVCLDTCHTFTAGYDLRTKEACDHTFAEFDRIVGMHYLRAMHINDSKAEFASR
VDRHHSLGKGEIGWDCFEYIASDSRFNGIPLILETIDSTIWKEEIQQLRMFHRSATAVSEEA

Sequences:

>Translated_302_residues
MTNNNSSSKPETNIKFIGAHVSAAGGVDQAPMRAREIGANAFALFTKNQRQWAAKPLEAKTISAFKANCKMLGFSAEHIL
PHDSYLINLGAPEEEKLEKSRAAFIDEMERCNQLGLTLLNFHPGSHLKKISESECLAKIAESINLAHQAVPDVIAVIENT
SGQGTNLGWKFEQLAEIIDQVEDKSRVGVCLDTCHTFTAGYDLRTKEACDHTFAEFDRIVGMHYLRAMHINDSKAEFASR
VDRHHSLGKGEIGWDCFEYIASDSRFNGIPLILETIDSTIWKEEIQQLRMFHRSATAVSEEA
>Mature_301_residues
TNNNSSSKPETNIKFIGAHVSAAGGVDQAPMRAREIGANAFALFTKNQRQWAAKPLEAKTISAFKANCKMLGFSAEHILP
HDSYLINLGAPEEEKLEKSRAAFIDEMERCNQLGLTLLNFHPGSHLKKISESECLAKIAESINLAHQAVPDVIAVIENTS
GQGTNLGWKFEQLAEIIDQVEDKSRVGVCLDTCHTFTAGYDLRTKEACDHTFAEFDRIVGMHYLRAMHINDSKAEFASRV
DRHHSLGKGEIGWDCFEYIASDSRFNGIPLILETIDSTIWKEEIQQLRMFHRSATAVSEEA

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=276, Percent_Identity=67.3913043478261, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17531193, Length=265, Percent_Identity=47.1698113207547, Blast_Score=277, Evalue=6e-75,
Organism=Saccharomyces cerevisiae, GI6322735, Length=260, Percent_Identity=42.6923076923077, Blast_Score=230, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 33623; Mature: 33492

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNNSSSKPETNIKFIGAHVSAAGGVDQAPMRAREIGANAFALFTKNQRQWAAKPLEAK
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCHHHHCCCCCHH
TISAFKANCKMLGFSAEHILPHDSYLINLGAPEEEKLEKSRAAFIDEMERCNQLGLTLLN
HHHHHHHCCEEECCCHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
FHPGSHLKKISESECLAKIAESINLAHQAVPDVIAVIENTSGQGTNLGWKFEQLAEIIDQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
VEDKSRVGVCLDTCHTFTAGYDLRTKEACDHTFAEFDRIVGMHYLRAMHINDSKAEFASR
HHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VDRHHSLGKGEIGWDCFEYIASDSRFNGIPLILETIDSTIWKEEIQQLRMFHRSATAVSE
HHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EA
CC
>Mature Secondary Structure 
TNNNSSSKPETNIKFIGAHVSAAGGVDQAPMRAREIGANAFALFTKNQRQWAAKPLEAK
CCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCHHHHCCCCCHH
TISAFKANCKMLGFSAEHILPHDSYLINLGAPEEEKLEKSRAAFIDEMERCNQLGLTLLN
HHHHHHHCCEEECCCHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
FHPGSHLKKISESECLAKIAESINLAHQAVPDVIAVIENTSGQGTNLGWKFEQLAEIIDQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
VEDKSRVGVCLDTCHTFTAGYDLRTKEACDHTFAEFDRIVGMHYLRAMHINDSKAEFASR
HHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VDRHHSLGKGEIGWDCFEYIASDSRFNGIPLILETIDSTIWKEEIQQLRMFHRSATAVSE
HHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA