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The map label for this gene is hlyB [H]

Identifier: 218708452

GI number: 218708452

Start: 421710

End: 422966

Strand: Direct

Name: hlyB [H]

Synonym: VS_0415

Alternate gene names: 218708452

Gene position: 421710-422966 (Clockwise)

Preceding gene: 218708450

Following gene: 218708453

Centisome position: 12.78

GC content: 46.94

Gene sequence:

>1257_bases
ATGACTAAACTGTCATTCAAGCCGTGGGAAAGGATTATCTCGGACATCCGTCTCGTTCCAAAAATGATCATGCTGATGGT
TTTCAGCACTATCTTGATTATTTCGAAGCAGCTATGGGACGCAAGTGCGTTTTACGATTCATTACTTGCAGTCACTAACA
ATGCACAAGTTGCACAGCAGCATTACGAGACTTACCTAGTTCAAGTGGCTTGGCAAACAGCCTTGATGATCATTGTGTTT
GTGGTTCTTCTATTGGCGGCAGCGCGCGTTATGCTTCGCCAAACTCAATACCTCAATGACGCCATTAAAACGATGGCCGA
TAAGAACCTATCAGTGCCTATTCATATGGATTGTAAAGATGAATACGGTGATGTGGCACGAGAACTCGAAAAAACTCGAG
TCCAGTTGAATGACATGATCAAAACTCAGGTGGCCTCTTCTGATGAGTTGTTTGCCCTGACGGAAGTGATGACCATCAGC
ATGTCCGAAACCAAAGATTCAGCTCAGGAAGAATTTAACGAAATCGACCAACTAGCGACAGCAATGAGCGAGATGACGTC
TACTGTGCAAACTGTGGCTGAGCACGCGCAAAGTGCTTCTTCTCTTACTGAGCAAGCATCAGGTCAAGCACTAACAGGTC
AGAAGTTCGTTCAAGGTTCTGTTTCTAAGATGAGTGAGCTTTCTTCGGACATCGCAGCCTCTGCAGCAGCGGTAAACCAA
GTAGAAGAGCGCGTAGACTCGATTGGCAGTGTGGTTGGTACTATCCAAGGCATTTCAGAGCAAACGAACCTATTGGCTTT
GAACGCAGCGATTGAAGCCGCGCGTGCCGGTGAAGCGGGACGTGGTTTTGCGGTTGTTGCCGATGAGGTTCGTAACCTTG
CGCAACGTACTCAGCAAGCGACAGTAGAAATTCAAGATATGATTAGCCATCTACAAAGCAGTGCTAACTCGGCTGTAGAG
CTGATGGAAAAGAGTGTTGTTGAAGCCGCTGAAGGCGTAGACCTAGTGACTAACGCTGGTTCTGAGCTTGATGGCATCGT
AAGTCAGGTAAACCAGATTAATGATATGAACTTCCAGATCGCGACTGCTGCGGGCCAACAAACTAGTGTCGCGGAAGAGA
TGAGTGTCAACCTGACCAATGTTCGTGAGCTTGTTGAAGCTTCCGTAGTGGTGGTGGGTGAGCTTCTCGAGACTTCTGAG
ATCATGGAAAGCAACGCACAAGAGCTAGACGGTAAGATTAAGCAGTTTAAGGTTTAA

Upstream 100 bases:

>100_bases
CATTCATACGAGGCTCAAAATAAATCGCGAACTTTGACTGACATAGCCATCTCGCGGATATAGAAGCCATCAATATATAA
GAGTTAATGGAGAACTGACA

Downstream 100 bases:

>100_bases
TCCCCTGTTCTCGGTCTTGAGCTCTAGTTCGCAAGATCTATAGGTCTATTCCTAGAGGTTTGGCCACGGAGACTTGTTAT
TAGAGATAAAGGCTTTGCTT

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MTKLSFKPWERIISDIRLVPKMIMLMVFSTILIISKQLWDASAFYDSLLAVTNNAQVAQQHYETYLVQVAWQTALMIIVF
VVLLLAAARVMLRQTQYLNDAIKTMADKNLSVPIHMDCKDEYGDVARELEKTRVQLNDMIKTQVASSDELFALTEVMTIS
MSETKDSAQEEFNEIDQLATAMSEMTSTVQTVAEHAQSASSLTEQASGQALTGQKFVQGSVSKMSELSSDIAASAAAVNQ
VEERVDSIGSVVGTIQGISEQTNLLALNAAIEAARAGEAGRGFAVVADEVRNLAQRTQQATVEIQDMISHLQSSANSAVE
LMEKSVVEAAEGVDLVTNAGSELDGIVSQVNQINDMNFQIATAAGQQTSVAEEMSVNLTNVRELVEASVVVVGELLETSE
IMESNAQELDGKIKQFKV

Sequences:

>Translated_418_residues
MTKLSFKPWERIISDIRLVPKMIMLMVFSTILIISKQLWDASAFYDSLLAVTNNAQVAQQHYETYLVQVAWQTALMIIVF
VVLLLAAARVMLRQTQYLNDAIKTMADKNLSVPIHMDCKDEYGDVARELEKTRVQLNDMIKTQVASSDELFALTEVMTIS
MSETKDSAQEEFNEIDQLATAMSEMTSTVQTVAEHAQSASSLTEQASGQALTGQKFVQGSVSKMSELSSDIAASAAAVNQ
VEERVDSIGSVVGTIQGISEQTNLLALNAAIEAARAGEAGRGFAVVADEVRNLAQRTQQATVEIQDMISHLQSSANSAVE
LMEKSVVEAAEGVDLVTNAGSELDGIVSQVNQINDMNFQIATAAGQQTSVAEEMSVNLTNVRELVEASVVVVGELLETSE
IMESNAQELDGKIKQFKV
>Mature_417_residues
TKLSFKPWERIISDIRLVPKMIMLMVFSTILIISKQLWDASAFYDSLLAVTNNAQVAQQHYETYLVQVAWQTALMIIVFV
VLLLAAARVMLRQTQYLNDAIKTMADKNLSVPIHMDCKDEYGDVARELEKTRVQLNDMIKTQVASSDELFALTEVMTISM
SETKDSAQEEFNEIDQLATAMSEMTSTVQTVAEHAQSASSLTEQASGQALTGQKFVQGSVSKMSELSSDIAASAAAVNQV
EERVDSIGSVVGTIQGISEQTNLLALNAAIEAARAGEAGRGFAVVADEVRNLAQRTQQATVEIQDMISHLQSSANSAVEL
MEKSVVEAAEGVDLVTNAGSELDGIVSQVNQINDMNFQIATAAGQQTSVAEEMSVNLTNVRELVEASVVVVGELLETSEI
MESNAQELDGKIKQFKV

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1788195, Length=267, Percent_Identity=34.0823970037453, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI2367378, Length=298, Percent_Identity=31.8791946308725, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1787690, Length=294, Percent_Identity=31.6326530612245, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788194, Length=345, Percent_Identity=30.7246376811594, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1789453, Length=345, Percent_Identity=28.9855072463768, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 45428; Mature: 45297

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLSFKPWERIISDIRLVPKMIMLMVFSTILIISKQLWDASAFYDSLLAVTNNAQVAQQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
HYETYLVQVAWQTALMIIVFVVLLLAAARVMLRQTQYLNDAIKTMADKNLSVPIHMDCKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCH
EYGDVARELEKTRVQLNDMIKTQVASSDELFALTEVMTISMSETKDSAQEEFNEIDQLAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMSEMTSTVQTVAEHAQSASSLTEQASGQALTGQKFVQGSVSKMSELSSDIAASAAAVNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEERVDSIGSVVGTIQGISEQTNLLALNAAIEAARAGEAGRGFAVVADEVRNLAQRTQQA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
TVEIQDMISHLQSSANSAVELMEKSVVEAAEGVDLVTNAGSELDGIVSQVNQINDMNFQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCCCEEE
ATAAGQQTSVAEEMSVNLTNVRELVEASVVVVGELLETSEIMESNAQELDGKIKQFKV
EECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHCCC
>Mature Secondary Structure 
TKLSFKPWERIISDIRLVPKMIMLMVFSTILIISKQLWDASAFYDSLLAVTNNAQVAQQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
HYETYLVQVAWQTALMIIVFVVLLLAAARVMLRQTQYLNDAIKTMADKNLSVPIHMDCKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCH
EYGDVARELEKTRVQLNDMIKTQVASSDELFALTEVMTISMSETKDSAQEEFNEIDQLAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMSEMTSTVQTVAEHAQSASSLTEQASGQALTGQKFVQGSVSKMSELSSDIAASAAAVNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEERVDSIGSVVGTIQGISEQTNLLALNAAIEAARAGEAGRGFAVVADEVRNLAQRTQQA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
TVEIQDMISHLQSSANSAVELMEKSVVEAAEGVDLVTNAGSELDGIVSQVNQINDMNFQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCCCEEE
ATAAGQQTSVAEEMSVNLTNVRELVEASVVVVGELLETSEIMESNAQELDGKIKQFKV
EECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]