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The map label for this gene is leuO [H]

Identifier: 218708424

GI number: 218708424

Start: 389145

End: 390104

Strand: Direct

Name: leuO [H]

Synonym: VS_0386

Alternate gene names: 218708424

Gene position: 389145-390104 (Clockwise)

Preceding gene: 218708423

Following gene: 218708425

Centisome position: 11.79

GC content: 44.38

Gene sequence:

>960_bases
ATGTTAGAGAAAAAAGACGCAATGAGTGCAATTGCAAGCTACAGAATGGAAAGCACACTTCGTGGAGTCGACTTAAATCT
TTTGACTGTATTTGATGCAGTTATGCAAGAGCAAAATATTACACGTGCAGCTCATAATCTGGGTATGTCTCAGCCAGCGG
TAAGTAATGCTGTTGCTCGTTTGAAAGTAATGTTCAACGACGAGCTATTCATGCGTCAAGGTCGTGGTATTCAACCGACT
CAACGTGCTCGTCAGTTGTTTGGCCCAATCCGCCAAGCGCTGCAGTTAGTACGCAACGAATTACCAAGTTCTGTATTCTC
TCCAGAGTCGTCTTCTCGTCTGTTCAAACTTGCAATTTGTAGCCCTTGCGACATGCGTTTTGCACCTAAGATTATGTCGA
CAATCAACGACCAAGCACCTAGCGTTAATCTGCACATGGATGCAGAATTTGACCGTCAACTTTCTGAGCGCATGCGTTAC
CAAGAAATTGACTTCGTTATTGATTACTCGCGTTTTGATGAACAAGGTTTCTCAAGCACTGAAATCTTCCAAGATGAATT
GGTTGTGGTAGCTTCTGCTTCTCACCCACGCATCAACGGTGAAGTTTCAGCGGCAGAATTGCTTAACGAAAAGCACGCTA
AACTGTCTCGCATTCACGGTCAACGTAGCTTCTCTGAGCAAGCTTACCGTGACCTAGATTGCACGCCTTTCTACGAAGGT
ACGAGCTTGAGCAACGTTCTTTACGTTGTTGGTCAATCTGAATTAGTAACAATCGCTCCTCGTTGGATGGTAGAACATGC
AGCAAACAAAGAGCAGCTTCAGATTTTAGATTTCCCATTCGATAATGCGGCGATTTCTGGCTTCCTTAGCTGGCACGAAT
CAAGTGAAAAGGACAAAGGACACATCTGGTTACGAGATCAGTTAATGATGATCTGTGGCGAAGTAGTAGCGCTTAACTAG

Upstream 100 bases:

>100_bases
AATCCGAAAAACATCGGTCTGATAAATATAAGAATTAGTGGTTATCTATTCCCACACCATTACCAATTTAACCCTGTCGA
ACCGACAAGAGGCAACTTGA

Downstream 100 bases:

>100_bases
ACACTAATTCCTATGATTTATAAGCCCTAAGCTAGGAGTCTGGCTTGGGGTTTTTTTGTACCTGCTATTTTTGTTGATAG
AAAACTTAGCGTGAGACGTA

Product: leucine transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MLEKKDAMSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVARLKVMFNDELFMRQGRGIQPT
QRARQLFGPIRQALQLVRNELPSSVFSPESSSRLFKLAICSPCDMRFAPKIMSTINDQAPSVNLHMDAEFDRQLSERMRY
QEIDFVIDYSRFDEQGFSSTEIFQDELVVVASASHPRINGEVSAAELLNEKHAKLSRIHGQRSFSEQAYRDLDCTPFYEG
TSLSNVLYVVGQSELVTIAPRWMVEHAANKEQLQILDFPFDNAAISGFLSWHESSEKDKGHIWLRDQLMMICGEVVALN

Sequences:

>Translated_319_residues
MLEKKDAMSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVARLKVMFNDELFMRQGRGIQPT
QRARQLFGPIRQALQLVRNELPSSVFSPESSSRLFKLAICSPCDMRFAPKIMSTINDQAPSVNLHMDAEFDRQLSERMRY
QEIDFVIDYSRFDEQGFSSTEIFQDELVVVASASHPRINGEVSAAELLNEKHAKLSRIHGQRSFSEQAYRDLDCTPFYEG
TSLSNVLYVVGQSELVTIAPRWMVEHAANKEQLQILDFPFDNAAISGFLSWHESSEKDKGHIWLRDQLMMICGEVVALN
>Mature_319_residues
MLEKKDAMSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVARLKVMFNDELFMRQGRGIQPT
QRARQLFGPIRQALQLVRNELPSSVFSPESSSRLFKLAICSPCDMRFAPKIMSTINDQAPSVNLHMDAEFDRQLSERMRY
QEIDFVIDYSRFDEQGFSSTEIFQDELVVVASASHPRINGEVSAAELLNEKHAKLSRIHGQRSFSEQAYRDLDCTPFYEG
TSLSNVLYVVGQSELVTIAPRWMVEHAANKEQLQILDFPFDNAAISGFLSWHESSEKDKGHIWLRDQLMMICGEVVALN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=295, Percent_Identity=52.8813559322034, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI87081766, Length=270, Percent_Identity=25.9259259259259, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786820, Length=291, Percent_Identity=24.0549828178694, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36160; Mature: 36160

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKKDAMSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVAR
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LKVMFNDELFMRQGRGIQPTQRARQLFGPIRQALQLVRNELPSSVFSPESSSRLFKLAIC
HHHEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEEEC
SPCDMRFAPKIMSTINDQAPSVNLHMDAEFDRQLSERMRYQEIDFVIDYSRFDEQGFSST
CCCCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCHH
EIFQDELVVVASASHPRINGEVSAAELLNEKHAKLSRIHGQRSFSEQAYRDLDCTPFYEG
HHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCC
TSLSNVLYVVGQSELVTIAPRWMVEHAANKEQLQILDFPFDNAAISGFLSWHESSEKDKG
CCCCCEEEEECCCCEEEECHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCC
HIWLRDQLMMICGEVVALN
EEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLEKKDAMSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVAR
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LKVMFNDELFMRQGRGIQPTQRARQLFGPIRQALQLVRNELPSSVFSPESSSRLFKLAIC
HHHEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEEEC
SPCDMRFAPKIMSTINDQAPSVNLHMDAEFDRQLSERMRYQEIDFVIDYSRFDEQGFSST
CCCCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCHH
EIFQDELVVVASASHPRINGEVSAAELLNEKHAKLSRIHGQRSFSEQAYRDLDCTPFYEG
HHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCC
TSLSNVLYVVGQSELVTIAPRWMVEHAANKEQLQILDFPFDNAAISGFLSWHESSEKDKG
CCCCCEEEEECCCCEEEECHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCC
HIWLRDQLMMICGEVVALN
EEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]