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The map label for this gene is dhfrIII [H]

Identifier: 218708405

GI number: 218708405

Start: 366120

End: 366599

Strand: Direct

Name: dhfrIII [H]

Synonym: VS_0367

Alternate gene names: 218708405

Gene position: 366120-366599 (Clockwise)

Preceding gene: 218708404

Following gene: 218708412

Centisome position: 11.1

GC content: 44.17

Gene sequence:

>480_bases
ATGATCATCAGCATGATAGCGGCAATGGCGAACAACCGTGTAATTGGTAAAGACAATCAGATGCCTTGGCATTTACCTGC
AGATTTCGCATGGTTTAAACGTTCAACTATGGGTAAACCTGTCGTGATGGGACGTAAGACTTACGATTCAATTGGACGTC
CTTTACCGGGGAGGCTGAACGTTGTAATCAGCCGCGACGAGAGTTTAGAAATCGGAGGCGTCACGACGGTAACTTCGATT
GAAAAAGCACTAGAGCTGGTTAGTGATGTTGATGAAGTGATGATCATCGGTGGCGGTTCAATCTATGAAAGCTGCTTACC
AAAAGCGAACAAGTTATACCTGACTTATATCGATTTTGATGTGGATGGTGATACTCAATTCCCAGACTGGGGAGAAGGTT
GGAAGCAGAGCTTTAATGATACTTATCAGGCGGATGAGAAAAACAAGCACAATATGGAGTTTGTGGTTCTCGAGCGTTAA

Upstream 100 bases:

>100_bases
CTTGCGGTTGGCTTAGCAGTGCCTGGGCTGTTATTCTACCGTCGTAGACCTATCGTCTAGCTTCAACTTCATTTTCAAGT
AAGGACAGGACTTTCGATTT

Downstream 100 bases:

>100_bases
CGGTGGTTATTAAGCATTAGGCTCTGAAAATGCTTGCGTTACTGTGCCCAGCTAGCGCAAGCTTCTCGTATCTCGTATCT
CGTATCTCGTATCTCGTATC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MIISMIAAMANNRVIGKDNQMPWHLPADFAWFKRSTMGKPVVMGRKTYDSIGRPLPGRLNVVISRDESLEIGGVTTVTSI
EKALELVSDVDEVMIIGGGSIYESCLPKANKLYLTYIDFDVDGDTQFPDWGEGWKQSFNDTYQADEKNKHNMEFVVLER

Sequences:

>Translated_159_residues
MIISMIAAMANNRVIGKDNQMPWHLPADFAWFKRSTMGKPVVMGRKTYDSIGRPLPGRLNVVISRDESLEIGGVTTVTSI
EKALELVSDVDEVMIIGGGSIYESCLPKANKLYLTYIDFDVDGDTQFPDWGEGWKQSFNDTYQADEKNKHNMEFVVLER
>Mature_159_residues
MIISMIAAMANNRVIGKDNQMPWHLPADFAWFKRSTMGKPVVMGRKTYDSIGRPLPGRLNVVISRDESLEIGGVTTVTSI
EKALELVSDVDEVMIIGGGSIYESCLPKANKLYLTYIDFDVDGDTQFPDWGEGWKQSFNDTYQADEKNKHNMEFVVLER

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=147, Percent_Identity=37.4149659863946, Blast_Score=74, Evalue=4e-14,
Organism=Homo sapiens, GI28827793, Length=149, Percent_Identity=34.2281879194631, Blast_Score=64, Evalue=8e-11,
Organism=Escherichia coli, GI1786233, Length=159, Percent_Identity=53.4591194968553, Blast_Score=180, Evalue=3e-47,
Organism=Drosophila melanogaster, GI116008026, Length=177, Percent_Identity=31.638418079096, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24647458, Length=177, Percent_Identity=31.638418079096, Blast_Score=83, Evalue=8e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17925; Mature: 17925

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIISMIAAMANNRVIGKDNQMPWHLPADFAWFKRSTMGKPVVMGRKTYDSIGRPLPGRLN
CHHHHHHHHHCCEEECCCCCCCEECCCCHHHHHHCCCCCCEEECCCCHHHHCCCCCCCEE
VVISRDESLEIGGVTTVTSIEKALELVSDVDEVMIIGGGSIYESCLPKANKLYLTYIDFD
EEEECCCCEEECCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHCCCCCEEEEEEEEEE
VDGDTQFPDWGEGWKQSFNDTYQADEKNKHNMEFVVLER
CCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MIISMIAAMANNRVIGKDNQMPWHLPADFAWFKRSTMGKPVVMGRKTYDSIGRPLPGRLN
CHHHHHHHHHCCEEECCCCCCCEECCCCHHHHHHCCCCCCEEECCCCHHHHCCCCCCCEE
VVISRDESLEIGGVTTVTSIEKALELVSDVDEVMIIGGGSIYESCLPKANKLYLTYIDFD
EEEECCCCEEECCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHCCCCCEEEEEEEEEE
VDGDTQFPDWGEGWKQSFNDTYQADEKNKHNMEFVVLER
CCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]