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The map label for this gene is pucG [H]

Identifier: 218708358

GI number: 218708358

Start: 308365

End: 309504

Strand: Direct

Name: pucG [H]

Synonym: VS_0318

Alternate gene names: 218708358

Gene position: 308365-309504 (Clockwise)

Preceding gene: 218708356

Following gene: 218708363

Centisome position: 9.35

GC content: 46.49

Gene sequence:

>1140_bases
ATGTCTAATTTAACACTCACTACTGCTATCGATAGCTTTTATCCGCCACACCGAACGTTAATGGGGCCGGGGCCTTCAGA
CATTTCTCCTCAAGTTTTACAGGCCTTAAGCCGTCCAACCATTGGTCACCTCGACCCGCTATTTATTGCGATGATGGATG
AGCTTAAACAACTTCTTAAGTATGCATTCCAAACAGAGAATGAGTTTACGATTGCTGTTTCTGCTCCAGGCAGTGCGGGC
ATGGAAACCTGTTTTGTTAATTTGATTGAGCCTGGTGAAAAGGTCATTGTTTGTCGTAACGGTGTTTTTGGTGAGCGTAT
GCGCGAGAATGTTGTTCGCTGCGGTGGTGAAGCCATCCTTGTTGATGACGAGTGGGGTAAGCCCGTTTCAGTTGAAAAGG
TAGAAAAGGCATTGGCAGAAAACCCCGATGCTGTTGCTGTCGCATTTGTGCATGCCGAGACGTCTACTGGCGCGTTATCG
GATGCTCAAGCTATTTCAGCAGTAGCTCGTCAGTTTGATGCATTGTCGATTGTGGATGCGGTGACATCATTAGGTGGTGT
GCCATTGTTGGTTGATGAATGGCAACTCGATGCGGTTTATTCAGGCAGTCAGAAGTGCCTGTCTTGTGTACCTGGTTTAT
CTCCCGTGACGTTCTCTCAACGTGCTGTCGATAAAATGAAAGCACGTCAAGCTCCCGTACAAAGCTGGTTCTTGGATCAG
AGCTTGGTATTAGGTTATTGGAGTGGAGAAGGTAAGCGTAGCTATCACCACACTGCGCCTGTGAACAGCTTATATGCTCT
GCATGAGTCGTTGGTTTTGTTGAAAAATGAAGGCCTAGACAATGCGTGGTCTCGTCACTATGCGATGCATCAAGAACTTA
AAGCTGGCGTAGAAGCTTTGGGGTTGGAATTTGTGGTTGCTGAAGAGAGTCGCTTACCTCAATTGAATGCGCTTTACTTC
CCTGAAGGGATTGATGAAGCCAAAATAAGAACGCAGCTGTTAGAAGAGTATAATCTTGAAATCGGTGCTGGGCTGGGCTC
TCTGGCTGGAAAGGCTTGGCGTATTGGTCTGATGGGCTATGGTGCTCGTAAAGAGAATGTCGCTTTGTGCTTAAAAGCGT
TACAAGACGTTTTAAAATAA

Upstream 100 bases:

>100_bases
TTATGTGACACTGGTTTGACCTCAATACTTATGGCTAATTGCTGAGGTAATCAATTTGCTTTAGGGTGGATTGTTCACAA
CATTAACGTAAGGTATGTAC

Downstream 100 bases:

>100_bases
CGCTAATAAATTGAAGTTTAAAAGAAAAGCGCACGAGAGAATGTGCGCTTTTTTGTGTCTAGGCTATAGGCTAAACGTCA
TTACAGCAAAATGACTCTAG

Product: serine--pyruvate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSNLTLTTAIDSFYPPHRTLMGPGPSDISPQVLQALSRPTIGHLDPLFIAMMDELKQLLKYAFQTENEFTIAVSAPGSAG
METCFVNLIEPGEKVIVCRNGVFGERMRENVVRCGGEAILVDDEWGKPVSVEKVEKALAENPDAVAVAFVHAETSTGALS
DAQAISAVARQFDALSIVDAVTSLGGVPLLVDEWQLDAVYSGSQKCLSCVPGLSPVTFSQRAVDKMKARQAPVQSWFLDQ
SLVLGYWSGEGKRSYHHTAPVNSLYALHESLVLLKNEGLDNAWSRHYAMHQELKAGVEALGLEFVVAEESRLPQLNALYF
PEGIDEAKIRTQLLEEYNLEIGAGLGSLAGKAWRIGLMGYGARKENVALCLKALQDVLK

Sequences:

>Translated_379_residues
MSNLTLTTAIDSFYPPHRTLMGPGPSDISPQVLQALSRPTIGHLDPLFIAMMDELKQLLKYAFQTENEFTIAVSAPGSAG
METCFVNLIEPGEKVIVCRNGVFGERMRENVVRCGGEAILVDDEWGKPVSVEKVEKALAENPDAVAVAFVHAETSTGALS
DAQAISAVARQFDALSIVDAVTSLGGVPLLVDEWQLDAVYSGSQKCLSCVPGLSPVTFSQRAVDKMKARQAPVQSWFLDQ
SLVLGYWSGEGKRSYHHTAPVNSLYALHESLVLLKNEGLDNAWSRHYAMHQELKAGVEALGLEFVVAEESRLPQLNALYF
PEGIDEAKIRTQLLEEYNLEIGAGLGSLAGKAWRIGLMGYGARKENVALCLKALQDVLK
>Mature_378_residues
SNLTLTTAIDSFYPPHRTLMGPGPSDISPQVLQALSRPTIGHLDPLFIAMMDELKQLLKYAFQTENEFTIAVSAPGSAGM
ETCFVNLIEPGEKVIVCRNGVFGERMRENVVRCGGEAILVDDEWGKPVSVEKVEKALAENPDAVAVAFVHAETSTGALSD
AQAISAVARQFDALSIVDAVTSLGGVPLLVDEWQLDAVYSGSQKCLSCVPGLSPVTFSQRAVDKMKARQAPVQSWFLDQS
LVLGYWSGEGKRSYHHTAPVNSLYALHESLVLLKNEGLDNAWSRHYAMHQELKAGVEALGLEFVVAEESRLPQLNALYFP
EGIDEAKIRTQLLEEYNLEIGAGLGSLAGKAWRIGLMGYGARKENVALCLKALQDVLK

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=366, Percent_Identity=36.0655737704918, Blast_Score=249, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI17536281, Length=360, Percent_Identity=38.6111111111111, Blast_Score=258, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6321079, Length=386, Percent_Identity=28.7564766839378, Blast_Score=109, Evalue=6e-25,
Organism=Drosophila melanogaster, GI17530823, Length=371, Percent_Identity=38.544474393531, Blast_Score=265, Evalue=6e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.-

Molecular weight: Translated: 41203; Mature: 41071

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLTLTTAIDSFYPPHRTLMGPGPSDISPQVLQALSRPTIGHLDPLFIAMMDELKQLLK
CCCCEEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
YAFQTENEFTIAVSAPGSAGMETCFVNLIEPGEKVIVCRNGVFGERMRENVVRCGGEAIL
HHHCCCCCEEEEEECCCCCCHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHCCCCEEE
VDDEWGKPVSVEKVEKALAENPDAVAVAFVHAETSTGALSDAQAISAVARQFDALSIVDA
EECCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
VTSLGGVPLLVDEWQLDAVYSGSQKCLSCVPGLSPVTFSQRAVDKMKARQAPVQSWFLDQ
HHHHCCCEEEEECCEECHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCC
SLVLGYWSGEGKRSYHHTAPVNSLYALHESLVLLKNEGLDNAWSRHYAMHQELKAGVEAL
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHH
GLEFVVAEESRLPQLNALYFPEGIDEAKIRTQLLEEYNLEIGAGLGSLAGKAWRIGLMGY
CEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCEECCCHHHHCCCEEEEEEEEC
GARKENVALCLKALQDVLK
CCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SNLTLTTAIDSFYPPHRTLMGPGPSDISPQVLQALSRPTIGHLDPLFIAMMDELKQLLK
CCCEEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
YAFQTENEFTIAVSAPGSAGMETCFVNLIEPGEKVIVCRNGVFGERMRENVVRCGGEAIL
HHHCCCCCEEEEEECCCCCCHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHCCCCEEE
VDDEWGKPVSVEKVEKALAENPDAVAVAFVHAETSTGALSDAQAISAVARQFDALSIVDA
EECCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
VTSLGGVPLLVDEWQLDAVYSGSQKCLSCVPGLSPVTFSQRAVDKMKARQAPVQSWFLDQ
HHHHCCCEEEEECCEECHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCC
SLVLGYWSGEGKRSYHHTAPVNSLYALHESLVLLKNEGLDNAWSRHYAMHQELKAGVEAL
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHH
GLEFVVAEESRLPQLNALYFPEGIDEAKIRTQLLEEYNLEIGAGLGSLAGKAWRIGLMGY
CEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCEECCCHHHHCCCEEEEEEEEC
GARKENVALCLKALQDVLK
CCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]