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The map label for this gene is dosC [H]

Identifier: 218708303

GI number: 218708303

Start: 237672

End: 239087

Strand: Direct

Name: dosC [H]

Synonym: VS_0241

Alternate gene names: 218708303

Gene position: 237672-239087 (Clockwise)

Preceding gene: 218708302

Following gene: 218708306

Centisome position: 7.2

GC content: 42.73

Gene sequence:

>1416_bases
GTGGTTTTTAGCTTGTTACTTGTTGATTCAGCGGAAGAGTCAGAAAAGCAGTATGGAATTGAAAACCAAGGCGTGACACG
CTCTTTAGCGGAGCTTACACAGATTATTAATGCGCTTGAATATAATATTACCGCACTTTATCCGCTGCATGGGGATACCT
ACGTATTCTCACATGAGCAAAAGGTCGAAGGTGATACGTGTTACTTCATTAGTGAAGAGCAAGCTGCCCCTCGATTTGAT
TTTATGTTCTCTGGCCCAAGTGAGATGTGCGATCTAAAGTCGGAGCTTCATTTAGAAGCAGGTAGGCGCATGTTTATCGC
GCCCACCATGGCTTACTTTGCGAATACCATTGATACCATCTCTGCGATTTACTTTATCTCCAAAGATAAATTTATTATAT
CGTCGCCTTCCGATTTCGCCCGTTACATTAAAGGTGACACCTTTGATTCAGTCGTCAATAGTCGCCCTTATTGGGTGAAT
ACGATTCGTTTTGGCTTAGCTCAGGGTCAAGATCAAGTGGTGTATACCGGCCAATACGATGACTACCTAACAGGGAAGAA
GGTAGTGACCTTAACCAAAGGTATCTATGTTGATGGTGAGTTTAAAGGAGTACTCGGTGTTGATGGCTACGTCTCTAATC
TCGTCTCGAATCCCGTTCATGGATACAAGGTGACGAGCGCGAGAGGAGTAAACAAATATGGCCTGTTGGACTTTACCTAT
TCACGGCCTTTGTACGTCGATGGAATTAATACTGAGTTATACCTGACTATTGAAGAAGGAAAGAGTGAGCACTTCTTACA
TTTGCTGGAACTGGAGCGGGTTCAATTACTGATTTTGTTAGCTTTGTTCTTTATCTCAGCACTATGGTTGTGGCGGTTTT
ATATCAAGCAAGAGCACAAGCGATTGAAGAATTTAGCGATGCTCGACCCTTTGACTGGTTTATATAATCGGCGTGGTTTT
GAAGTTCGGTTTCTGAACCAAAATGAGGAACCAATTATTGGTGTTGGCGTATTTGATATCGATGATTTCAAAGTGGTGAA
TGACCAGTATGGTCATAAGGTGGGAGATGAGGTGATTTGTCATGTTGCTCAGTTAATGCTGAACAGTGTTAGGCAGCAAG
ATATCGTGGCGAGATTTGGTGGTGAAGAGTTTGTTGTCGCGATTATGGGCGAATCATCCGAATTACTTTCATCGATCTTT
GAACGAATTCAAAATGACATTAGTTTGCAAAGTTATCGATGTGCGACCGGTGACAAGATTAATATCTCAGTCTCCGGGGG
AGCGACGCTTTACTCTCTGTACAAGTTCGACAGCATTGGACACTTGTGGGAAAACCAGAGTATTCGTTCATCAGATGAGC
AACTTTATAAGGCGAAGGCCTCAGGCAAGAATCAAGTGTCTATTCACGTACATTAA

Upstream 100 bases:

>100_bases
CAATGTGCGCTAAATTTTCAACGATGACTTATATGCTTTGATTAATCCCCATACCGTCGATTTTAAAAAGGCTTTCTTAA
GTCTGACCGCCAGTTTTTTA

Downstream 100 bases:

>100_bases
TCTGTGAACTGCAGTTTAGAGCCTAGTCGTTTGCTAGGCTCTTTTTATCTAAGGAAAAAGCCCTTTAAGATGAAACAGTA
GCTAAGGATAAAATGCAGCT

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MVFSLLLVDSAEESEKQYGIENQGVTRSLAELTQIINALEYNITALYPLHGDTYVFSHEQKVEGDTCYFISEEQAAPRFD
FMFSGPSEMCDLKSELHLEAGRRMFIAPTMAYFANTIDTISAIYFISKDKFIISSPSDFARYIKGDTFDSVVNSRPYWVN
TIRFGLAQGQDQVVYTGQYDDYLTGKKVVTLTKGIYVDGEFKGVLGVDGYVSNLVSNPVHGYKVTSARGVNKYGLLDFTY
SRPLYVDGINTELYLTIEEGKSEHFLHLLELERVQLLILLALFFISALWLWRFYIKQEHKRLKNLAMLDPLTGLYNRRGF
EVRFLNQNEEPIIGVGVFDIDDFKVVNDQYGHKVGDEVICHVAQLMLNSVRQQDIVARFGGEEFVVAIMGESSELLSSIF
ERIQNDISLQSYRCATGDKINISVSGGATLYSLYKFDSIGHLWENQSIRSSDEQLYKAKASGKNQVSIHVH

Sequences:

>Translated_471_residues
MVFSLLLVDSAEESEKQYGIENQGVTRSLAELTQIINALEYNITALYPLHGDTYVFSHEQKVEGDTCYFISEEQAAPRFD
FMFSGPSEMCDLKSELHLEAGRRMFIAPTMAYFANTIDTISAIYFISKDKFIISSPSDFARYIKGDTFDSVVNSRPYWVN
TIRFGLAQGQDQVVYTGQYDDYLTGKKVVTLTKGIYVDGEFKGVLGVDGYVSNLVSNPVHGYKVTSARGVNKYGLLDFTY
SRPLYVDGINTELYLTIEEGKSEHFLHLLELERVQLLILLALFFISALWLWRFYIKQEHKRLKNLAMLDPLTGLYNRRGF
EVRFLNQNEEPIIGVGVFDIDDFKVVNDQYGHKVGDEVICHVAQLMLNSVRQQDIVARFGGEEFVVAIMGESSELLSSIF
ERIQNDISLQSYRCATGDKINISVSGGATLYSLYKFDSIGHLWENQSIRSSDEQLYKAKASGKNQVSIHVH
>Mature_471_residues
MVFSLLLVDSAEESEKQYGIENQGVTRSLAELTQIINALEYNITALYPLHGDTYVFSHEQKVEGDTCYFISEEQAAPRFD
FMFSGPSEMCDLKSELHLEAGRRMFIAPTMAYFANTIDTISAIYFISKDKFIISSPSDFARYIKGDTFDSVVNSRPYWVN
TIRFGLAQGQDQVVYTGQYDDYLTGKKVVTLTKGIYVDGEFKGVLGVDGYVSNLVSNPVHGYKVTSARGVNKYGLLDFTY
SRPLYVDGINTELYLTIEEGKSEHFLHLLELERVQLLILLALFFISALWLWRFYIKQEHKRLKNLAMLDPLTGLYNRRGF
EVRFLNQNEEPIIGVGVFDIDDFKVVNDQYGHKVGDEVICHVAQLMLNSVRQQDIVARFGGEEFVVAIMGESSELLSSIF
ERIQNDISLQSYRCATGDKINISVSGGATLYSLYKFDSIGHLWENQSIRSSDEQLYKAKASGKNQVSIHVH

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=167, Percent_Identity=33.5329341317365, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786584, Length=179, Percent_Identity=31.2849162011173, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082007, Length=244, Percent_Identity=30.7377049180328, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787262, Length=171, Percent_Identity=34.5029239766082, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI87081881, Length=183, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1787541, Length=176, Percent_Identity=30.6818181818182, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081974, Length=336, Percent_Identity=25, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=31.4917127071823, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787816, Length=189, Percent_Identity=30.1587301587302, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787056, Length=169, Percent_Identity=32.5443786982249, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081977, Length=125, Percent_Identity=36, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787802, Length=185, Percent_Identity=29.1891891891892, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788381, Length=183, Percent_Identity=31.1475409836066, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 53426; Mature: 53426

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFSLLLVDSAEESEKQYGIENQGVTRSLAELTQIINALEYNITALYPLHGDTYVFSHEQ
CEEEEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEECCC
KVEGDTCYFISEEQAAPRFDFMFSGPSEMCDLKSELHLEAGRRMFIAPTMAYFANTIDTI
CCCCCEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHH
SAIYFISKDKFIISSPSDFARYIKGDTFDSVVNSRPYWVNTIRFGLAQGQDQVVYTGQYD
HEEEEEECCCEEEECCHHHHHHHCCCCHHHHHCCCCCEEEEEEEHHCCCCCCEEEECCCC
DYLTGKKVVTLTKGIYVDGEFKGVLGVDGYVSNLVSNPVHGYKVTSARGVNKYGLLDFTY
CCCCCCEEEEEECCEEECCCCCEEEECCHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEC
SRPLYVDGINTELYLTIEEGKSEHFLHLLELERVQLLILLALFFISALWLWRFYIKQEHK
CCCEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLKNLAMLDPLTGLYNRRGFEVRFLNQNEEPIIGVGVFDIDDFKVVNDQYGHKVGDEVIC
HHHHHHHHCCHHHHHCCCCCEEEEECCCCCCEEEEEEEECCCEEEECCCCCCHHHHHHHH
HVAQLMLNSVRQQDIVARFGGEEFVVAIMGESSELLSSIFERIQNDISLQSYRCATGDKI
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCEE
NISVSGGATLYSLYKFDSIGHLWENQSIRSSDEQLYKAKASGKNQVSIHVH
EEEECCCCEEEEEHHHCCHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure
MVFSLLLVDSAEESEKQYGIENQGVTRSLAELTQIINALEYNITALYPLHGDTYVFSHEQ
CEEEEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEECCC
KVEGDTCYFISEEQAAPRFDFMFSGPSEMCDLKSELHLEAGRRMFIAPTMAYFANTIDTI
CCCCCEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHH
SAIYFISKDKFIISSPSDFARYIKGDTFDSVVNSRPYWVNTIRFGLAQGQDQVVYTGQYD
HEEEEEECCCEEEECCHHHHHHHCCCCHHHHHCCCCCEEEEEEEHHCCCCCCEEEECCCC
DYLTGKKVVTLTKGIYVDGEFKGVLGVDGYVSNLVSNPVHGYKVTSARGVNKYGLLDFTY
CCCCCCEEEEEECCEEECCCCCEEEECCHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEC
SRPLYVDGINTELYLTIEEGKSEHFLHLLELERVQLLILLALFFISALWLWRFYIKQEHK
CCCEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLKNLAMLDPLTGLYNRRGFEVRFLNQNEEPIIGVGVFDIDDFKVVNDQYGHKVGDEVIC
HHHHHHHHCCHHHHHCCCCCEEEEECCCCCCEEEEEEEECCCEEEECCCCCCHHHHHHHH
HVAQLMLNSVRQQDIVARFGGEEFVVAIMGESSELLSSIFERIQNDISLQSYRCATGDKI
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCEE
NISVSGGATLYSLYKFDSIGHLWENQSIRSSDEQLYKAKASGKNQVSIHVH
EEEECCCCEEEEEHHHCCHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]