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The map label for this gene is bioH

Identifier: 218708214

GI number: 218708214

Start: 143342

End: 144118

Strand: Direct

Name: bioH

Synonym: VS_0150

Alternate gene names: 218708214

Gene position: 143342-144118 (Clockwise)

Preceding gene: 218708209

Following gene: 218708215

Centisome position: 4.34

GC content: 48.78

Gene sequence:

>777_bases
ATGAGTGACACGTTATATTGGCATGTTTCTGGGCAAGGGCCTGATTTAGTGTTGGTTCACGGCTGGGGAATGAACGGTGC
GGTATGGCAACAAACCGTTGATGCGTTAGAAGCTGATTTTCGAGTGCATGTTGTTGACCTTCCTGGGTATGGACACAGCG
CGCATTGCCACGCTCAAGATCTTGAAGAGATAGCTCAGCAATTGTTGGCCGAAGCTCCAAAGCGGGCTATTTGGGTCGGT
TGGTCACTGGGTGGCTTGGTCGCAACACACATGGCTTTGCATCACTCAGATTACGTAAGCAAATTGGTGACTGTTGCCAG
TTCTCCTAAATTCGCCGCTGCAAAAGAACCCGTGTTATGGCGAGGTATTCAGCCAAACGTATTAAGCGCATTTACCGAAC
AGCTGGTTGAAGATTTCCAAACCACTATCGAACGCTTTATGGCACTGCAAGCCATGGGCAGCCCTTCTGCAAGGCAAGAT
GTAAAACAACTCAAGCAAGCGGTACTGTCTCGTCCGCTTCCGAATCCTGATTCTTTATTGGCCGGCTTAAAGATGCTGTC
TGATGTTGACTTGCGTGAACAACTGCCGGAGATTTCAGTGCCTATGCTGCGTTTATACGGGCGACTGGATGGATTGGTGC
CAATCAAGGTAGCAAAAGATCTGGGTAGAGCGCTGCCTCATACCGAGCAATATATATTCACGCAGTCTTCACATGCGCCG
TTTATGACAGAAGCCGATGCCTTTTGCAGTGAACTAGTTAGCTTCGCGCAAAAATAA

Upstream 100 bases:

>100_bases
ATAACATAGCTCGTGATGCTTTTGGGTTATGGTGTTCCTTTTACTCACAATACTGAAAAGCGCGGATTATCGCTGATGGA
AAAAGTAGGGAATATCGAGA

Downstream 100 bases:

>100_bases
TCACTAAATTTATTGCCGGATTGGTCGATATATAACCTAATACCAATCACAGTAAGTAAGTGTTCAAAAACAGCGTAGGA
AAAAGGCTTGAGAACAAGGC

Product: carboxylesterase BioH

Products: NA

Alternate protein names: Biotin synthesis protein BioH

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSDTLYWHVSGQGPDLVLVHGWGMNGAVWQQTVDALEADFRVHVVDLPGYGHSAHCHAQDLEEIAQQLLAEAPKRAIWVG
WSLGGLVATHMALHHSDYVSKLVTVASSPKFAAAKEPVLWRGIQPNVLSAFTEQLVEDFQTTIERFMALQAMGSPSARQD
VKQLKQAVLSRPLPNPDSLLAGLKMLSDVDLREQLPEISVPMLRLYGRLDGLVPIKVAKDLGRALPHTEQYIFTQSSHAP
FMTEADAFCSELVSFAQK

Sequences:

>Translated_258_residues
MSDTLYWHVSGQGPDLVLVHGWGMNGAVWQQTVDALEADFRVHVVDLPGYGHSAHCHAQDLEEIAQQLLAEAPKRAIWVG
WSLGGLVATHMALHHSDYVSKLVTVASSPKFAAAKEPVLWRGIQPNVLSAFTEQLVEDFQTTIERFMALQAMGSPSARQD
VKQLKQAVLSRPLPNPDSLLAGLKMLSDVDLREQLPEISVPMLRLYGRLDGLVPIKVAKDLGRALPHTEQYIFTQSSHAP
FMTEADAFCSELVSFAQK
>Mature_257_residues
SDTLYWHVSGQGPDLVLVHGWGMNGAVWQQTVDALEADFRVHVVDLPGYGHSAHCHAQDLEEIAQQLLAEAPKRAIWVGW
SLGGLVATHMALHHSDYVSKLVTVASSPKFAAAKEPVLWRGIQPNVLSAFTEQLVEDFQTTIERFMALQAMGSPSARQDV
KQLKQAVLSRPLPNPDSLLAGLKMLSDVDLREQLPEISVPMLRLYGRLDGLVPIKVAKDLGRALPHTEQYIFTQSSHAPF
MTEADAFCSELVSFAQK

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family

Homologues:

Organism=Escherichia coli, GI1789817, Length=257, Percent_Identity=50.1945525291829, Blast_Score=262, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOH_VIBSL (B7VHH1)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002415835.1
- ProteinModelPortal:   B7VHH1
- GeneID:   7159823
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0150
- HOGENOM:   HBG748003
- OMA:   HWPQKER
- ProtClustDB:   CLSK874891
- GO:   GO:0005737
- HAMAP:   MF_01260
- InterPro:   IPR000073
- InterPro:   IPR010076
- TIGRFAMs:   TIGR01738

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.1.1.1

Molecular weight: Translated: 28457; Mature: 28326

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: ACT_SITE 82-82 ACT_SITE 210-210 ACT_SITE 238-238

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTLYWHVSGQGPDLVLVHGWGMNGAVWQQTVDALEADFRVHVVDLPGYGHSAHCHAQD
CCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHH
LEEIAQQLLAEAPKRAIWVGWSLGGLVATHMALHHSDYVSKLVTVASSPKFAAAKEPVLW
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
RGIQPNVLSAFTEQLVEDFQTTIERFMALQAMGSPSARQDVKQLKQAVLSRPLPNPDSLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
AGLKMLSDVDLREQLPEISVPMLRLYGRLDGLVPIKVAKDLGRALPHTEQYIFTQSSHAP
HHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHEEEECCCCCC
FMTEADAFCSELVSFAQK
CCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDTLYWHVSGQGPDLVLVHGWGMNGAVWQQTVDALEADFRVHVVDLPGYGHSAHCHAQD
CCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHH
LEEIAQQLLAEAPKRAIWVGWSLGGLVATHMALHHSDYVSKLVTVASSPKFAAAKEPVLW
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
RGIQPNVLSAFTEQLVEDFQTTIERFMALQAMGSPSARQDVKQLKQAVLSRPLPNPDSLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
AGLKMLSDVDLREQLPEISVPMLRLYGRLDGLVPIKVAKDLGRALPHTEQYIFTQSSHAP
HHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHEEEECCCCCC
FMTEADAFCSELVSFAQK
CCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA