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The map label for this gene is fadA [H]

Identifier: 218708127

GI number: 218708127

Start: 37501

End: 38664

Strand: Reverse

Name: fadA [H]

Synonym: VS_0040

Alternate gene names: 218708127

Gene position: 38664-37501 (Counterclockwise)

Preceding gene: 218708128

Following gene: 218708126

Centisome position: 1.17

GC content: 49.4

Gene sequence:

>1164_bases
ATGAAAAATGTAGTTGTTGTTGATTGCCTTCGTACCCCAATGGGACGCTCCAAAGGTGGAGCTTTCCGTCACACTCGTGC
TGAAGATCTGTCTGCACATTTGATGAAAGGCATTTTAGAGCGCAACCCAGAAGTAAACCCTGTCGAAATTGAAGACATTT
ACTGGGGCTGTGTACAACAAACGCTAGAGCAAGGCTTCAACGTTGCCCGTAACGCAGCTTTGCTTGCTGGTCTACCGATT
GAGATTGGTGCGGTGACGGTCAACCGTTTATGTGGTTCATCTATGCAAGCTCTGCATGATGCAACCCGCTCTATCATGGT
GGGTGATGCTGAAATCTGCCTGATCGGTGGTGTCGAACATATGGGTCACGTACCAATGAACCATGGTGTTGATTTTCACC
CTGGCATGTCAAAACACGTAGCAAAAGCGGCGGGTATGATGGGTCTAACCGCTGAGATGTTAGGTAAAATGCACGGTATT
AGCCGTGAAGACCAAGATGCATTCGCAGCACGCTCACATGCTCGCGCTCAAGCAGCTACGGTTGAAGGTCGTTTCAAAAA
TGAAATCCTGCCAACCGAAGCTCACGCTGCAGACGGTTCTCTGTTCACTCTGGATTACGACGAAGTCATTCGTCCAGAGA
CAACAGTTGAAGGCCTATCTCAGCTGCGTCCGGTGTTTGACCCAGCAAACGGCACAGTAACAGCAGGTACTTCATCAGCT
CTGTCTGATGGCGCTTCTGCAATGCTTATCATGAGCGAAGAGAAAGCCAACGCACTTGGCCTTAAGATTCGCGCTCGCGT
TAGGTCTATGGCTATCGCTGGCTGCGATCCTTCAATCATGGGTTATGGCCCAGTACCAGCAACCCAAAAAGCGCTGAAGC
GTGCAGGTTTAACGATTGAAGACATGGGTGTGATTGAGCTGAACGAAGCGTTTGCTGCTCAATCTCTTCCATGTGCGAAA
GACCTAGGTTTACTGGATGTGGTTGACGAGAAAGTAAACCTTAACGGCGGTGCCATTGCTCTTGGTCACCCACTAGGCTG
TTCTGGTTCTCGTATCTCTACAACCCTAATCAACCTGATGGAAGCCCAAGACGTGAAATACGGCCTGGCGACTATGTGTA
TTGGTTTAGGCCAAGGTATTGCAACGGTATTTGAACGCCCATAG

Upstream 100 bases:

>100_bases
TTCCTCAACTATTGCTTGATATGGCAGCGAAGGGCGAAAGCTTTTACGACGGTCAACAAGCCAGTTCTCTGTAACCCACA
TTTGGAAAAGGAATAGCAAA

Downstream 100 bases:

>100_bases
CGTTTACTGACTGTAATACTTGAAGGGTAATCTTTTGTGCAGCTATATTTATATAGCTGCACTTTTTATTACTTGGCATC
CAACAATGCATCGATTGCGT

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKNVVVVDCLRTPMGRSKGGAFRHTRAEDLSAHLMKGILERNPEVNPVEIEDIYWGCVQQTLEQGFNVARNAALLAGLPI
EIGAVTVNRLCGSSMQALHDATRSIMVGDAEICLIGGVEHMGHVPMNHGVDFHPGMSKHVAKAAGMMGLTAEMLGKMHGI
SREDQDAFAARSHARAQAATVEGRFKNEILPTEAHAADGSLFTLDYDEVIRPETTVEGLSQLRPVFDPANGTVTAGTSSA
LSDGASAMLIMSEEKANALGLKIRARVRSMAIAGCDPSIMGYGPVPATQKALKRAGLTIEDMGVIELNEAFAAQSLPCAK
DLGLLDVVDEKVNLNGGAIALGHPLGCSGSRISTTLINLMEAQDVKYGLATMCIGLGQGIATVFERP

Sequences:

>Translated_387_residues
MKNVVVVDCLRTPMGRSKGGAFRHTRAEDLSAHLMKGILERNPEVNPVEIEDIYWGCVQQTLEQGFNVARNAALLAGLPI
EIGAVTVNRLCGSSMQALHDATRSIMVGDAEICLIGGVEHMGHVPMNHGVDFHPGMSKHVAKAAGMMGLTAEMLGKMHGI
SREDQDAFAARSHARAQAATVEGRFKNEILPTEAHAADGSLFTLDYDEVIRPETTVEGLSQLRPVFDPANGTVTAGTSSA
LSDGASAMLIMSEEKANALGLKIRARVRSMAIAGCDPSIMGYGPVPATQKALKRAGLTIEDMGVIELNEAFAAQSLPCAK
DLGLLDVVDEKVNLNGGAIALGHPLGCSGSRISTTLINLMEAQDVKYGLATMCIGLGQGIATVFERP
>Mature_387_residues
MKNVVVVDCLRTPMGRSKGGAFRHTRAEDLSAHLMKGILERNPEVNPVEIEDIYWGCVQQTLEQGFNVARNAALLAGLPI
EIGAVTVNRLCGSSMQALHDATRSIMVGDAEICLIGGVEHMGHVPMNHGVDFHPGMSKHVAKAAGMMGLTAEMLGKMHGI
SREDQDAFAARSHARAQAATVEGRFKNEILPTEAHAADGSLFTLDYDEVIRPETTVEGLSQLRPVFDPANGTVTAGTSSA
LSDGASAMLIMSEEKANALGLKIRARVRSMAIAGCDPSIMGYGPVPATQKALKRAGLTIEDMGVIELNEAFAAQSLPCAK
DLGLLDVVDEKVNLNGGAIALGHPLGCSGSRISTTLINLMEAQDVKYGLATMCIGLGQGIATVFERP

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=398, Percent_Identity=40.2010050251256, Blast_Score=264, Evalue=8e-71,
Organism=Homo sapiens, GI4501853, Length=394, Percent_Identity=41.1167512690355, Blast_Score=245, Evalue=6e-65,
Organism=Homo sapiens, GI148539872, Length=396, Percent_Identity=37.8787878787879, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI4557237, Length=397, Percent_Identity=34.5088161209068, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI4504327, Length=432, Percent_Identity=34.7222222222222, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI194353979, Length=240, Percent_Identity=32.9166666666667, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI48994986, Length=386, Percent_Identity=76.4248704663213, Blast_Score=622, Evalue=1e-180,
Organism=Escherichia coli, GI1787663, Length=405, Percent_Identity=44.6913580246914, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI87082165, Length=399, Percent_Identity=42.1052631578947, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI1788554, Length=400, Percent_Identity=43.5, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1788683, Length=421, Percent_Identity=32.3040380047506, Blast_Score=178, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI133906874, Length=400, Percent_Identity=39, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17535921, Length=405, Percent_Identity=35.8024691358025, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=432, Percent_Identity=31.9444444444444, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI25147385, Length=397, Percent_Identity=31.7380352644836, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17535917, Length=392, Percent_Identity=26.530612244898, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322031, Length=397, Percent_Identity=38.0352644836272, Blast_Score=223, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=34.75, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24655093, Length=400, Percent_Identity=40.25, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI17648125, Length=334, Percent_Identity=43.4131736526946, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=32.8671328671329, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24640423, Length=397, Percent_Identity=33.5012594458438, Blast_Score=181, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40909; Mature: 40909

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVVVVDCLRTPMGRSKGGAFRHTRAEDLSAHLMKGILERNPEVNPVEIEDIYWGCVQQ
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
TLEQGFNVARNAALLAGLPIEIGAVTVNRLCGSSMQALHDATRSIMVGDAEICLIGGVEH
HHHHHHHHHHCCHHEECCCEEEHHHHHHHHHCHHHHHHHHHHHHEEECCCCEEEEECHHH
MGHVPMNHGVDFHPGMSKHVAKAAGMMGLTAEMLGKMHGISREDQDAFAARSHARAQAAT
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHE
VEGRFKNEILPTEAHAADGSLFTLDYDEVIRPETTVEGLSQLRPVFDPANGTVTAGTSSA
ECCHHHCCCCCCCCCCCCCCEEEECHHHHHCCCHHHHHHHHHCCCCCCCCCEEECCCCHH
LSDGASAMLIMSEEKANALGLKIRARVRSMAIAGCDPSIMGYGPVPATQKALKRAGLTIE
HCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHCCCEEE
DMGVIELNEAFAAQSLPCAKDLGLLDVVDEKVNLNGGAIALGHPLGCSGSRISTTLINLM
CCCEEEECHHHHHCCCCCHHCCCHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHH
EAQDVKYGLATMCIGLGQGIATVFERP
HHHHHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MKNVVVVDCLRTPMGRSKGGAFRHTRAEDLSAHLMKGILERNPEVNPVEIEDIYWGCVQQ
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
TLEQGFNVARNAALLAGLPIEIGAVTVNRLCGSSMQALHDATRSIMVGDAEICLIGGVEH
HHHHHHHHHHCCHHEECCCEEEHHHHHHHHHCHHHHHHHHHHHHEEECCCCEEEEECHHH
MGHVPMNHGVDFHPGMSKHVAKAAGMMGLTAEMLGKMHGISREDQDAFAARSHARAQAAT
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHE
VEGRFKNEILPTEAHAADGSLFTLDYDEVIRPETTVEGLSQLRPVFDPANGTVTAGTSSA
ECCHHHCCCCCCCCCCCCCCEEEECHHHHHCCCHHHHHHHHHCCCCCCCCCEEECCCCHH
LSDGASAMLIMSEEKANALGLKIRARVRSMAIAGCDPSIMGYGPVPATQKALKRAGLTIE
HCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHCCCEEE
DMGVIELNEAFAAQSLPCAKDLGLLDVVDEKVNLNGGAIALGHPLGCSGSRISTTLINLM
CCCEEEECHHHHHCCCCCHHCCCHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHH
EAQDVKYGLATMCIGLGQGIATVFERP
HHHHHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]