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The map label for this gene is yoaA [H]

Identifier: 218705306

GI number: 218705306

Start: 2124756

End: 2126666

Strand: Reverse

Name: yoaA [H]

Synonym: ECUMN_2100

Alternate gene names: 218705306

Gene position: 2126666-2124756 (Counterclockwise)

Preceding gene: 218705309

Following gene: 218705305

Centisome position: 40.88

GC content: 55.21

Gene sequence:

>1911_bases
GTGACGGACGATTTTGCACCAGACGGTCAGTTGGCGAAAGCGATACCAGGCTTTAAGCCGCGAGAACCACAGCGACAGAT
GGCGGTAGCCGTCACCCAGGCGATAGAAAAAGGCCAGCCGCTGGTGGTGGAAGCCGGAACCGGTACGGGCAAAACCTACG
CTTACCTGGCCCCTGCGCTGCGGGCGAAAAAGAAAGTCATTATCTCGACCGGTTCAAAAGCGTTGCAGGATCAGCTCTAC
AGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCT
CTGCCTCGAGCGTCTCGAACAGCAGGCGCTGGCGGGGGGCGATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGC
GCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTG
GTCACCAGTACCAACGATAACTGCCTTGGCAGCGACTGCCCGATGTATAAAGAATGCTTTGTGGTCAAAGCACGCAAAAA
AGCGATGGACGCCGATGTGGTGGTGGTAAACCATCATCTCTTTCTGGCGGATATGGTGGTTAAGGAGAGTGGATTTGGCG
AGTTGATCCCGGAAGCGGACGTGATGATCTTCGACGAAGCCCACCAGCTACCGGACATTGCCAGCCAGTATTTTGGTCAG
TCACTCTCCAGTCGACAACTGCTCGACCTGGCAAAAGACATCACCATCGCCTACCGCACCGAATTAAAAGACACCCAGCA
GTTACAAAAGTGCACTGATCGTCTTGCCCAGAGTGCGCAGGATTTTCGTCTGCAATTAGGTGAGCCAGGTTATCGTGGCA
ACCTGCGCGAACTGTTAGCTAATCCGCAAATTCAGCGGGCGTTTTTACTGCTCGATGACACCCTGGAACTTTGTTATGAC
GTGGCGAAACTGTCGCTGGGGCGTTCCGCCTTGCTGGATGCGGCATTTGAGCGCGCCACGTTGTATCGCACGCGGCTGAA
ACGGCTAAAAGAGATCAATCAGCCGGGCTACAGCTACTGGTACGAATGCACTTCGCGCCATTTTACTCTGGCACTCACGC
CGCTCAGCGTGGCGGATAAATTCAAAGAGTTAATGGCGCAAAAACCAGGTAGCTGGATCTTTACCTCAGCAACGCTGTCG
GTGAATGACGATCTGCATCATTTTACCTCGCGGCTTGGCATTGAACAGGCAGAGTCGTTGCTGTTACCCAGCCCGTTTGA
TTACAGCCGCCAGGCGTTACTCTGTGTGCCGCGCAACCTGCCGCAAACCAATCAACCGGGCTCCGCACGGCAACTGGCGG
CAATGTTGCGACCGATCATCGAAGCTAACAACGGTCGTTGTTTTATGCTTTGTACCTCGCACGCCATGATGCGCGATCTG
GCTGAGCAGTTCCGCGCTACCATGACGCTTCCCGTTTTGTTGCAGGGGGAAACCAGCAAAGGGCAACTGTTGCAGCAATT
TGTCAGCGCCGGTAACGCGCTTCTTGTGGCAACCAGCAGCTTCTGGGAAGGGGTGGATGTGCGTGGCGATACATTGTCAT
TGGTGATTATCGACAAGTTGCCGTTTACCTCACCGGATGATCCACTATTAAAAGCGCGCATGGAAGATTGCCGTTTGCGC
GGAGGCGACCCGTTCGATGAAGTACAGCTGCCGGATGCGGTCATTACTCTCAAACAGGGGGTAGGGCGACTAATTCGCGA
CGCCGACGATCGAGGGGTTTTGGTGATTTGCGATAATCGGCTGGTGATGCGCCCTTACGGCGCGACGTTTCTCGCCAGTC
TGCCGCCAGCGCCGCGCACCCGTGACATTGCCCGTGCGGTTCGTTTCCTTGCGATACCATCCTCCAGGTAA

Upstream 100 bases:

>100_bases
GCTGCAAAGACTGCCACAATGTGAGGTCGCCGTCATCACTTCAACTGGCGAAAGCGCCCTCATCCTGACATAATCCCTGT
TCAAATCAACAGGGGGTAGT

Downstream 100 bases:

>100_bases
TTTGTGATGGAGTATGGTATGATGCGCGCCTTTTTATTGATCTAAGCACGAAGACCCATGCGAATTCTGGCTATCGATAC
CGCGACAGAGGCCTGCTCTG

Product: putative ATP-dependent helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 636; Mature: 635

Protein sequence:

>636_residues
MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVEAGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLY
SRDLPTVSKALKYTGNVALLKGRSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
VTSTNDNCLGSDCPMYKECFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPEADVMIFDEAHQLPDIASQYFGQ
SLSSRQLLDLAKDITIAYRTELKDTQQLQKCTDRLAQSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYD
VAKLSLGRSALLDAAFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSWIFTSATLS
VNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSARQLAAMLRPIIEANNGRCFMLCTSHAMMRDL
AEQFRATMTLPVLLQGETSKGQLLQQFVSAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLR
GGDPFDEVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIARAVRFLAIPSSR

Sequences:

>Translated_636_residues
MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVEAGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLY
SRDLPTVSKALKYTGNVALLKGRSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
VTSTNDNCLGSDCPMYKECFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPEADVMIFDEAHQLPDIASQYFGQ
SLSSRQLLDLAKDITIAYRTELKDTQQLQKCTDRLAQSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYD
VAKLSLGRSALLDAAFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSWIFTSATLS
VNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSARQLAAMLRPIIEANNGRCFMLCTSHAMMRDL
AEQFRATMTLPVLLQGETSKGQLLQQFVSAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLR
GGDPFDEVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIARAVRFLAIPSSR
>Mature_635_residues
TDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVEAGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYS
RDLPTVSKALKYTGNVALLKGRSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPLV
TSTNDNCLGSDCPMYKECFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPEADVMIFDEAHQLPDIASQYFGQS
LSSRQLLDLAKDITIAYRTELKDTQQLQKCTDRLAQSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDV
AKLSLGRSALLDAAFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSWIFTSATLSV
NDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSARQLAAMLRPIIEANNGRCFMLCTSHAMMRDLA
EQFRATMTLPVLLQGETSKGQLLQQFVSAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRG
GDPFDEVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIARAVRFLAIPSSR

Specific function: Unknown

COG id: COG1199

COG function: function code KL; Rad3-related DNA helicases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase ATP-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788110, Length=636, Percent_Identity=99.5283018867924, Blast_Score=1296, Evalue=0.0,
Organism=Escherichia coli, GI1787018, Length=702, Percent_Identity=29.0598290598291, Blast_Score=206, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR010614
- InterPro:   IPR014013
- InterPro:   IPR006555
- InterPro:   IPR006935 [H]

Pfam domain/function: PF06733 DEAD_2; PF04851 ResIII [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 70407; Mature: 70276

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVEAGTGTGKTYAYLAPAL
CCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
RAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKGRSNYLCLERLEQQALAGG
HCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCC
DLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPLVTSTNDNCLGSDCPMYKECF
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHH
VVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPEADVMIFDEAHQLPDIASQYFGQ
HHHHHHHCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCEEEECCHHCCHHHHHHHHCC
SLSSRQLLDLAKDITIAYRTELKDTQQLQKCTDRLAQSAQDFRLQLGEPGYRGNLRELLA
CCCHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHCHHHHEEECCCCCCCCCHHHHHC
NPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDAAFERATLYRTRLKRLKEINQPGYSYW
CCCHHHEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
YECTSRHFTLALTPLSVADKFKELMAQKPGSWIFTSATLSVNDDLHHFTSRLGIEQAESL
HHHCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHCHHHHHHH
LLPSPFDYSRQALLCVPRNLPQTNQPGSARQLAAMLRPIIEANNGRCFMLCTSHAMMRDL
CCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHH
AEQFRATMTLPVLLQGETSKGQLLQQFVSAGNALLVATSSFWEGVDVRGDTLSLVIIDKL
HHHHHHHEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEEECC
PFTSPDDPLLKARMEDCRLRGGDPFDEVQLPDAVITLKQGVGRLIRDADDRGVLVICDNR
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCE
LVMRPYGATFLASLPPAPRTRDIARAVRFLAIPSSR
EEECCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVEAGTGTGKTYAYLAPAL
CCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
RAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKGRSNYLCLERLEQQALAGG
HCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCC
DLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPLVTSTNDNCLGSDCPMYKECF
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHH
VVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPEADVMIFDEAHQLPDIASQYFGQ
HHHHHHHCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCEEEECCHHCCHHHHHHHHCC
SLSSRQLLDLAKDITIAYRTELKDTQQLQKCTDRLAQSAQDFRLQLGEPGYRGNLRELLA
CCCHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHCHHHHEEECCCCCCCCCHHHHHC
NPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDAAFERATLYRTRLKRLKEINQPGYSYW
CCCHHHEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
YECTSRHFTLALTPLSVADKFKELMAQKPGSWIFTSATLSVNDDLHHFTSRLGIEQAESL
HHHCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHCHHHHHHH
LLPSPFDYSRQALLCVPRNLPQTNQPGSARQLAAMLRPIIEANNGRCFMLCTSHAMMRDL
CCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHH
AEQFRATMTLPVLLQGETSKGQLLQQFVSAGNALLVATSSFWEGVDVRGDTLSLVIIDKL
HHHHHHHEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEEECC
PFTSPDDPLLKARMEDCRLRGGDPFDEVQLPDAVITLKQGVGRLIRDADDRGVLVICDNR
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCE
LVMRPYGATFLASLPPAPRTRDIARAVRFLAIPSSR
EEECCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]