Click here to switch to the map view.

The map label for this gene is chbR [H]

Identifier: 218705233

GI number: 218705233

Start: 2053023

End: 2053865

Strand: Reverse

Name: chbR [H]

Synonym: ECUMN_2024

Alternate gene names: 218705233

Gene position: 2053865-2053023 (Counterclockwise)

Preceding gene: 218705234

Following gene: 218705232

Centisome position: 39.48

GC content: 41.28

Gene sequence:

>843_bases
ATGATGCAGCCAGTGATTAACGCGCCGGAAATTGCCACTGCCCGAGAACAGCAGTTGTTTAATGGCAAAAATTTCCATGT
GTTTATCTATAACAAAACTGAGAGTATCAGCGGACTGCATCAGCACGACTATTATGAATTTACTCTGGTATTAACCGGGC
GTTATTTCCAGGAGATTAACGGTAAGCGCGTGTTACTGGAACGGGGCGATTTTGTTTTTATTCCGTTAGGTTCGCACCAT
CAAAGTTTTTATGAGTTTGGTGCCACGCGCATATTGAACGTTGGGATCAGTAAACGCTTTTTTGAGCAGCATTACCTGCC
GTTGTTGCCTTATTGCTTTGTAGCTTCGCAGGTATACCGAACCAATAACGCTTTTCTCACCTATGTGGAAACAGTGATTT
CTTCATTGAATTTCCGCGAAACAGGGCTGGAAGAGTTTGTTGAGATGGTTACTTTTTATGTCATTAACCGTTTACGTCAT
TACCGCGAAGAACAGGTGATTGATGATGTACCGCAGTGGCTGAAAAGTACGGTAGAAAAGATGCATGATAAAGAGCAGTT
TAGTGAATCGGCGCTGGAGAATATGGTGACGTTGTCAGCCAAATCACAGGAATATTTGACGCGAGCGACTCAACGATATT
ATGGCAAAACACCAATGCAGATTATTAATGAGATCCGTATTAATTTTGCCAAAAAACAACTGGAAATGACCAACTATTCC
GTGACGGATATTGCGTTTGAGGCCGGTTATAGTAGCCCGAGTTTGTTTATTAAAACGTTTAAGAAATTAACGTCCTTTAC
GCCTAAGAGCTATCGTAAGAAATTGACTGAATTTAATCAGTAA

Upstream 100 bases:

>100_bases
CTGGTCCACGCTCAGGATCATTTAATGACGTCCATGCTTGCGCGTGAACTGATTACCGAATTAATTGAGCTTCATGAAAA
ACTGAAGGCATAAGGAGTCG

Downstream 100 bases:

>100_bases
GTCGTTATACCTGACAATTCACATATCTGTCCTGTTGCTGGATTATTTATGTCCGGGGGGGCAGATATGCCAGATATCAG
TATTCTGTACTGAAGGGAGA

Product: DNA-binding transcriptional regulator ChbR

Products: NA

Alternate protein names: Chb operon repressor [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHH
QSFYEFGATRILNVGISKRFFEQHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPMQIINEIRINFAKKQLEMTNYS
VTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTEFNQ

Sequences:

>Translated_280_residues
MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHH
QSFYEFGATRILNVGISKRFFEQHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPMQIINEIRINFAKKQLEMTNYS
VTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTEFNQ
>Mature_280_residues
MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHH
QSFYEFGATRILNVGISKRFFEQHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPMQIINEIRINFAKKQLEMTNYS
VTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTEFNQ

Specific function: Dual-function repressor/activator of the chbBCARFG operon. In the absence of the inducing sugar chitobiose, together with NagC, represses the chbBCARFG operon for the uptake and metabolism of chitobiose. In association with Crp, and probably in the presen

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788030, Length=280, Percent_Identity=99.6428571428571, Blast_Score=578, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 32998; Mature: 32998

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYFQEIN
CCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHCCCHHCCCEEEEEEEEHHHHHHCC
GKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQHYLPLLPYCFVASQVYR
CCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHH
TNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHYREEQVIDDVPQWLKSTVEK
CCCHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPMQIINEIRINFAKKQLEMTNYS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
VTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTEFNQ
HHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYFQEIN
CCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHCCCHHCCCEEEEEEEEHHHHHHCC
GKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQHYLPLLPYCFVASQVYR
CCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHH
TNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHYREEQVIDDVPQWLKSTVEK
CCCHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPMQIINEIRINFAKKQLEMTNYS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
VTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTEFNQ
HHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2179047; 9097039; 9278503; 9405618 [H]