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The map label for this gene is ydeH [H]

Identifier: 218705019

GI number: 218705019

Start: 1832474

End: 1833364

Strand: Reverse

Name: ydeH [H]

Synonym: ECUMN_1803

Alternate gene names: 218705019

Gene position: 1833364-1832474 (Counterclockwise)

Preceding gene: 218705020

Following gene: 218705017

Centisome position: 35.24

GC content: 44.22

Gene sequence:

>891_bases
ATGATCAAGAAGACAACGGAAATTGATGCCATCTTGTTAAATCTCAATAAGGCTATCGATGCCCACTACCAGTGGCTGGT
GAGTATGTTTCACAGCGTGGTCGCGAGAGATGCCAGTAAGCCAGAAATAACGGATAACCATTCTTATGGACTGTGCCAGT
TTGGTCGGTGGATTGATCATCTGGGACCACTCGATAACGATGAATTACCTTACGTTCGGCTAATGGATTCTGCCCATCAA
CATATGCATAACTGTGGTCGGGAATTAATGTTGGCTATTGTTGAAAATCACTGGCAGGACGCGCATTTCGACGCCTTTCA
GGAAGGGTTGCTTTCTTTTACTGCGGCATTAACCGATTACAAAATTTATTTGCTGACGATCCGTAGCAATATGGATGTTT
TGACGGGATTGCCGGGTCGTCGGGTTCTTGATGAATCCTTTGATCATCAGTTACGCAACGCTGAGCCTCTGAATCTTTAT
TTAATGTTGTTGGATATTGACCGATTTAAATTGGTTAATGATACCTACGGGCATTTAATCGGCGATGTAGTATTACGCAC
CCTGGCAACTTACTTAGCCAGTTGGACGCGCGATTACGAAACGGTTTATCGCTACGGTGGCGAAGAATTTATCATTATTG
TCAAAGCGGCTAATGATGAAGAAGCATGTCGTGCAGGTATCAGAATTTGCCAGTTAGTCGATAACCATGCCATCACACAT
TCTGAAGGGCATATCAACATTACCGTGACTGCAGGTGTGAGTCGCGCATTTCCTGAAGAACCTCTGGATGTGGTCATTGG
AAGAGCGGACCGGGCAATGTATGAGGGTAAGCAAACCGGAAGAAATCGCTGTATGTTTATTGACGAACAAAATGTGATTA
ACCGAGTTTAA

Upstream 100 bases:

>100_bases
AGATGACTCATAACTTCGGATTTCTATGAAAATATTGACGTAATTTTGCTTTTCTCTCGTTAGAATAGCGCGCACAAGGA
ACTGTGAAAAAGGAGTGGCA

Downstream 100 bases:

>100_bases
GCTCCGTTTAACATTCATTGAGAAAACTGATGCTACTGTGTCAACGATCCGTTATCAGTGCAGGAAAATGCCTGTTAGCG
TAAAAGCAAAACACAAATCT

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQ
HMHNCGRELMLAIVENHWQDAHFDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGIRICQLVDNHAITH
SEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV

Sequences:

>Translated_296_residues
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQ
HMHNCGRELMLAIVENHWQDAHFDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGIRICQLVDNHAITH
SEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV
>Mature_296_residues
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQ
HMHNCGRELMLAIVENHWQDAHFDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGIRICQLVDNHAITH
SEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV

Specific function: A diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returnes to normal when residues 206-207 are both mutated to Ala. Overexpression also leads to a reduction in flagellar abundance and a 20-fold increase in c-di- GMP

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787816, Length=296, Percent_Identity=99.6621621621622, Blast_Score=616, Evalue=1e-178,
Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=34.1614906832298, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=32.7485380116959, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787056, Length=191, Percent_Identity=32.4607329842932, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081881, Length=160, Percent_Identity=35.625, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787802, Length=180, Percent_Identity=30.5555555555556, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=29.6969696969697, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=31.7073170731707, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=33.1428571428571, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082007, Length=130, Percent_Identity=36.1538461538462, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=162, Percent_Identity=31.4814814814815, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33878; Mature: 33878

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDH
CCCCCHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
LGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAHFDAFQEGLLSFTAALTDY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
KIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLYLMLLDIDRFKLVNDTYGHLI
EEEEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHCHHHHHHH
GDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGIRICQLVDNHAITH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEE
SEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV
CCCEEEEEEEECCCCCCCCCCHHHEECCHHHHHHCCHHCCCCCEEEEECHHHHCCC
>Mature Secondary Structure
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDH
CCCCCHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
LGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAHFDAFQEGLLSFTAALTDY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
KIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLYLMLLDIDRFKLVNDTYGHLI
EEEEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHCHHHHHHH
GDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGIRICQLVDNHAITH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEE
SEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV
CCCEEEEEEEECCCCCCCCCCHHHEECCHHHHHHCCHHCCCCCEEEEECHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383113; 9097039; 9278503 [H]