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The map label for this gene is dhaH [H]

Identifier: 218704720

GI number: 218704720

Start: 1501945

End: 1503363

Strand: Reverse

Name: dhaH [H]

Synonym: ECUMN_1494

Alternate gene names: 218704720

Gene position: 1503363-1501945 (Counterclockwise)

Preceding gene: 218704721

Following gene: 218704719

Centisome position: 28.9

GC content: 50.53

Gene sequence:

>1419_bases
ATGGTAAACCTGGTCATAGTTTCACATAGCAGCCGACTGGGAGAAGGTGTCGGTGAACTAGCCCGTCAGATGTTAATGAG
TGATAGTTGTAAAATCGCCATTGCCGCGGGAATTGACGATCCACAAAATCCCATTGGTACCGATGCCGTCAAAGTGATGG
AGGCCATCGAATCTGTTGCCGATACCGACCATGTGCTGGTCATGATGGATATAGGAAGCGCATTATTGAGTGCTGAAACT
GCGCTGGAATTGCTGGCTCCCGAGATCGCCGCAAAAGTACGTTTGTGTGCTGCACCGTTGGTCGAAGGTACACTGGCAGC
AACGGTCAGCGCGGCCTCGGGGGCGGAGATCGACAAAGTTATCTTTGACGCCATGCATGCGCTGGAAGCCAAACGTGAAC
AACTGGGTTTACCCTCCTCCAACACTGAAATCTCTGACACATGTCCTCCGTACGATGAAGAAGCCCGTTCTCTGGCGGTG
GTCATAAAAAACCGTAACGGCCTGCATGTACGTCCGGCCTCCCGGCTGGTTTATACCTTATCGACATTTAATGCCGATAT
GTTGCTGGAGAAAAACGGCAAATGCGTCACACCGGAGAGTATTAACCAGATTGCGTTACTACAAGTTCGCTATAACGATA
CGCTGCGCCTGATTGCGAAAGGGCCAGAAGCTGAAGAGGCACTGATCGCTTTCCGTCAGCTGGCTGAAGATAACTTTGGT
GAAACGGAGGAAGTTGCTCCACCTATTCTGCGTCCCGTTCCGCCTGTTTCGGGTAAAGCCTTTTATTATCAACCGGTTTT
ATGTACGGTACAGGCAAAATCAACCCTGACCGTGGATGAAGAACAAGAACGATTACGCCAGGCCATTGACTTCACGTTAT
TAGATCTGATGACGTTAACAGCGAAAGCAGAAGCCAGCGGGCTTGACGATATTGCCGCAATCTTTTCTGGTCACCATACA
CTGTTAGATGATCCGGAACTGCTGGCGGCGGCAAGCGAACTCCTTCAGCATGAACATTGCACGGCAGAATATGCCTGGCA
GCAAGTTCTTAAAGAACTTAGCCAGCAATACCAGCAACTGGATGATGAATATCTACAGGCTCGCTATATTGATGTGGACG
ATCTTCTGCATCGCACCCTGGTCCACCTGACCCAAACGAAAGAAGAACTCCCGCAGTTTAACTCGCCAACTATTCTACTG
GCGGAGAACATCTATCCCTCCACAATACTGCAACTGGATCCGGCGGTTGTAAAAGGTATCTGCCTTAGCGCCGGAAGTCC
GCTATCCCACAGCGCCCTGATCGCCCGTGAACTGGGGATTGGCTGGATTTGCCAGCAGGGTGAGAAACTGTATGCGATAC
AACCAGAAGAAACGCTAACGCTGGACGTTAAAACGCAACGTTTCAGCCGTCAGGGTTAA

Upstream 100 bases:

>100_bases
CTCGGTGAACGCAGTATTGGTCACCAGGACCCCGGTGCGACCTCGGTGATGTTTATGATGCAAATGTTGGCGTTAGCCGC
AAAAGAGTAAGGAATTGGTG

Downstream 100 bases:

>100_bases
ACCACCGTTCGGATGGCATCATTCTGATGTCATCCGGCAATTTACTCTGCACGGATGTTTTTAACTACCTGTTATATTTT
TCACCAATTTGTGATGATTT

Product: dihydroxyacetone kinase subunit M

Products: NA

Alternate protein names: Phosphocarrier protein HPr; Protein H; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVADTDHVLVMMDIGSALLSAET
ALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPPYDEEARSLAV
VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTLTVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHT
LLDDPELLAAASELLQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILL
AENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFSRQG

Sequences:

>Translated_472_residues
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVADTDHVLVMMDIGSALLSAET
ALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPPYDEEARSLAV
VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTLTVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHT
LLDDPELLAAASELLQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILL
AENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFSRQG
>Mature_472_residues
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVADTDHVLVMMDIGSALLSAET
ALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPPYDEEARSLAV
VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTLTVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHT
LLDDPELLAAASELLQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILL
AENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFSRQG

Specific function: Phosphotransferase subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. DhaM serves as the phosphoryl donor. It is phosphorylated by HPr, then it donates its phosphoryl group to dhaL-ADP, which eventually tran

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI226510935, Length=472, Percent_Identity=97.6694915254237, Blast_Score=945, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=186, Percent_Identity=32.258064516129, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789193, Length=178, Percent_Identity=28.6516853932584, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI48994992, Length=310, Percent_Identity=23.8709677419355, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012844
- InterPro:   IPR008279
- InterPro:   IPR004701
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR008731 [H]

Pfam domain/function: PF03610 EIIA-man; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF00381 PTS-HPr [H]

EC number: NA

Molecular weight: Translated: 51513; Mature: 51513

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00369 PTS_HPR_HIS ; PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVA
CEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHC
DTDHVLVMMDIGSALLSAETALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGAEIDKV
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHH
IFDAMHALEAKREQLGLPSSNTEISDTCPPYDEEARSLAVVIKNRNGLHVRPASRLVYTL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCEEEEEEECCCCCEECCHHHEEEEE
STFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ECCCCEEEEECCCCEECCCCCCCEEEEEEEECCEEEEEECCCCHHHHHHHHHHHHCCCCC
ETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTLTVDEEQERLRQAIDFTLLDLMTLT
CCHHCCCCCCCCCCCCCCCEEEEECEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHH
AKAEASGLDDIAAIFSGHHTLLDDPELLAAASELLQHEHCTAEYAWQQVLKELSQQYQQL
HHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILLAENIYPSTILQLDPAVVKGI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCHHHHHHH
CLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFSRQG
HHCCCCCCCHHHHHHHHCCCCEEECCCCEEEEECCCCEEEEEEEHHHHCCCC
>Mature Secondary Structure
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVA
CEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHC
DTDHVLVMMDIGSALLSAETALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGAEIDKV
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHH
IFDAMHALEAKREQLGLPSSNTEISDTCPPYDEEARSLAVVIKNRNGLHVRPASRLVYTL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCEEEEEEECCCCCEECCHHHEEEEE
STFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ECCCCEEEEECCCCEECCCCCCCEEEEEEEECCEEEEEECCCCHHHHHHHHHHHHCCCCC
ETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTLTVDEEQERLRQAIDFTLLDLMTLT
CCHHCCCCCCCCCCCCCCCEEEEECEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHH
AKAEASGLDDIAAIFSGHHTLLDDPELLAAASELLQHEHCTAEYAWQQVLKELSQQYQQL
HHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILLAENIYPSTILQLDPAVVKGI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCHHHHHHH
CLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFSRQG
HHCCCCCCCHHHHHHHHCCCCEEECCCCEEEEECCCCEEEEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 2671658; 7984428 [H]