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The map label for this gene is ycgF [H]

Identifier: 218704676

GI number: 218704676

Start: 1460529

End: 1461740

Strand: Reverse

Name: ycgF [H]

Synonym: ECUMN_1448

Alternate gene names: 218704676

Gene position: 1461740-1460529 (Counterclockwise)

Preceding gene: 218704681

Following gene: 218704675

Centisome position: 28.1

GC content: 43.73

Gene sequence:

>1212_bases
ATGCTTACCACCCTTATTTATCGTAGCCATATACGTGACGACGAACCTGTCAAAAAAATCGAAGAAATGGTTTCGATAGC
AAATCGCAGGAACATGCAGTCTGACGTAACAGGGATCTTACTGTTTAATGGTTCTCATTTTTTCCAGCTTCTGGAAGGTC
CGGAAGAACAGGTTAAAATGATATATCGGGCTATATGCCAGGATCCACGGCACTATAATATTGTTGAGCTGCTGTGCGAT
TACGCGCCTGCTCGCCGTTTTGGCAAAGCGGGAATGGAATTATTTGATTTGCGCCTGCACGAGCGAGATGACGTTTTACA
GGCCGTATTCGACAAAGGCACATCAAAATTTCAGCTAACTTATGATGACAGAGCGCTACAATTTTTTCGTACTTTTGTCC
TTGCAACCGAACAAGCAACCTATTTCGAGATCCCTGCCGAAGACTCCTGGCTTTTTATCGCTGACGGATCTGATAAAGAA
CTTGATTCCTGTACCCTTTCACCAACTATAAACGACCACTTTGCCTTTCATCCTATTGTCGATCCCTTATCGCGGCGGAT
AATCGCTTTTGAAGCCATTGTGCAAAAAAATGAAGACAGCCCGTCAGCTATAGCGGTTGGGCAGCGTAAAGACGGGGAAA
TCTACAAAGCGGATCTCAAAAGTAAGGCGCTTGCATTCGCGATGGCACACGCACTTGACCTCGGTGATAAAATGATTTCA
ATCAATCTATTACCTATGACCCTGGTTAACGAACCTGACGCAGTCTCTTTTTTACTTGATGAAATAAAGGCCAATGCTCT
GGTGCCTGAACAAATCATCGTTGAATTTACTGAAAGTGAAGTCATATCTCGGTTTGATGAGTTTGCAGAAGCGATTAAAT
CGCTTAAGGCTGCCGGTATCAGTGTAGCAATTGATCATTTTGGCGCAGGTTTTGCCGGTTTGTTACTCCTGTCACGCTTC
CAGCCTGACAGAATTAAAATCAGTCAGGAATTGATTAACAATGTTCATAAAAGCGGGCCACGGCAGGCAATTATTCAGGC
GATCATAAAATGCTGTACATCACTTGAAATTCAAGTCTCTGCTATGGGTGTGACAACACCAGAAGAGTGGATGTGGCTTG
AATCTGCAGGAATTGAGATGTTTCAGGGAGATCTGTTTGCGAAAGCTAAATTGAATGGTATCCCTTCAGTTGCGTGGCCG
GAGAAAAAATAA

Upstream 100 bases:

>100_bases
AATGTAAAGAAACAGTTATGTTTTGGTATTAAAAGAAAGACACCGGAAGATATGTCTGTTACCGTCTTACTCTCGCCTCT
CCCATTACCCTGGACAGTTA

Downstream 100 bases:

>100_bases
TTTTCAGCACATTATTTCGTATGATTTCTGAAAATCATCGTGAAAATGAATACGTTATGTTTAAATTTAATCCACATTAA
AAATAATGTTTCATTCAATT

Product: putative cyclic-di-GMP phosphodiesterase

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNGSHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCD
YAPARRFGKAGMELFDLRLHERDDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKE
LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYKADLKSKALAFAMAHALDLGDKMIS
INLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEFTESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRF
QPDRIKISQELINNVHKSGPRQAIIQAIIKCCTSLEIQVSAMGVTTPEEWMWLESAGIEMFQGDLFAKAKLNGIPSVAWP
EKK

Sequences:

>Translated_403_residues
MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNGSHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCD
YAPARRFGKAGMELFDLRLHERDDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKE
LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYKADLKSKALAFAMAHALDLGDKMIS
INLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEFTESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRF
QPDRIKISQELINNVHKSGPRQAIIQAIIKCCTSLEIQVSAMGVTTPEEWMWLESAGIEMFQGDLFAKAKLNGIPSVAWP
EKK
>Mature_403_residues
MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNGSHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCD
YAPARRFGKAGMELFDLRLHERDDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKE
LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYKADLKSKALAFAMAHALDLGDKMIS
INLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEFTESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRF
QPDRIKISQELINNVHKSGPRQAIIQAIIKCCTSLEIQVSAMGVTTPEEWMWLESAGIEMFQGDLFAKAKLNGIPSVAWP
EKK

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=403, Percent_Identity=97.7667493796526, Blast_Score=816, Evalue=0.0,
Organism=Escherichia coli, GI1787541, Length=129, Percent_Identity=37.984496124031, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI226510982, Length=153, Percent_Identity=32.6797385620915, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788502, Length=223, Percent_Identity=27.8026905829596, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790496, Length=225, Percent_Identity=28, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789650, Length=121, Percent_Identity=26.4462809917355, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082096, Length=182, Percent_Identity=26.3736263736264, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081921, Length=152, Percent_Identity=28.9473684210526, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 45323; Mature: 45323

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50925 BLUF ; PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNGSHFFQLLEGPEEQVKM
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCHHHHHH
IYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHERDDVLQAVFDKGTSKFQLT
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEE
YDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKELDSCTLSPTINDHFAFHPIV
ECHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCCCCCCEECCCCCCCCCEECCHH
DPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYKADLKSKALAFAMAHALDLGDKMIS
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEE
INLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEFTESEVISRFDEFAEAIKSLKAAGI
EEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SVAIDHFGAGFAGLLLLSRFQPDRIKISQELINNVHKSGPRQAIIQAIIKCCTSLEIQVS
EEEEHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEE
AMGVTTPEEWMWLESAGIEMFQGDLFAKAKLNGIPSVAWPEKK
EEECCCHHHHEEEHHCCCHHHCCCCEEEHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNGSHFFQLLEGPEEQVKM
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCHHHHHH
IYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHERDDVLQAVFDKGTSKFQLT
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEE
YDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKELDSCTLSPTINDHFAFHPIV
ECHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCCCCCCEECCCCCCCCCEECCHH
DPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYKADLKSKALAFAMAHALDLGDKMIS
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEE
INLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEFTESEVISRFDEFAEAIKSLKAAGI
EEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SVAIDHFGAGFAGLLLLSRFQPDRIKISQELINNVHKSGPRQAIIQAIIKCCTSLEIQVS
EEEEHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEE
AMGVTTPEEWMWLESAGIEMFQGDLFAKAKLNGIPSVAWPEKK
EEECCCHHHHEEEHHCCCHHHCCCCEEEHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]