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The map label for this gene is rutD

Identifier: 218704426

GI number: 218704426

Start: 1243850

End: 1244689

Strand: Reverse

Name: rutD

Synonym: ECUMN_1192

Alternate gene names: 218704426

Gene position: 1244689-1243850 (Counterclockwise)

Preceding gene: 218704427

Following gene: 218704425

Centisome position: 23.93

GC content: 57.5

Gene sequence:

>840_bases
TTGCGCATATCGCCAAGTGAGGCCGCGATGAAACTTTCACTCTCACCTCCCCCTTATGCTGATGCGCCCGTAGTGGTGTT
GATTTCGGGTCTTGGCGGTAGCGGCAGTTACTGGTTACCGCAACTGGCGGTACTGGAGCAGGAGTATCAGGTAGTCTGTT
ACGACCAGCGCGGCACCGGCAATAATCCCGACACGCTGGCAGAAGATTACAGTATCGCCCAGATGGCAGCGGAACTGCAT
CAGGCGCTGGTAGCCGCAGGGATTAAGCGTTACGCGGTGGTCGGCCATGCGCTCGGTGCGCTGGTGGGAATGCAGCTGGC
GCTGGACTATCCCGCGTCGGTAACTATGCTGGTCAGCGTTAACGGCTGGCTACGAATAAACGCCCATACGCGCCGCTGTT
TTCAGGTTCGCGAACAGTTACTACATAGCGGCGGCGCGCAGGCATGGGTGGAAGCTCAGCCATTGTTCCTCTATCCCGCC
GACTGGATGGCGGCCCGCGCACCTCGCCTGGAAGCTGAAGACGCGCTGGCACTGGCGCATTTTCAGGGCAAAAATAATTT
ACTGCGTCGACTTAACGCCCTCAAACGCGCTGACTTTAGTCACCATGCGGATCGCATCCGCTGCCCGGTGCAAATCATCT
GCGCCAGTGATGATCTGCTGGTGCCATCAGCATGTTCCAGTGAACTTCATGCCGCCCTGCCCGATAGCCAGAAAATGGTG
ATGCGCTATGGCGGACACGCCTGCAACGTGACCCATCCCGAAACGTTTAATGCTCTGTTACTCAACGGGCTTGCCAGCCT
GTTACATCACCGTGAAGCCGCCTGTAAGGAATTGCTATGA

Upstream 100 bases:

>100_bases
AACGAAATCTACTCCGAGTTTTTCCCGGGTGACAAACCGGCGCGATTCTGCATTCAGTGCGGACTGGTAAAACCTGACGC
GCTGGTGGAAATCGCCACAA

Downstream 100 bases:

>100_bases
ACGAAGCCGTTAGCCCTGGTGCGCTTAGCACCCTATTCACCGATGCCCGCACTCACAACGGCTGGCGGGAGACACCCGTC
AGCGATGAGACGTTACGGGA

Product: alternative pyrimidine degradation pathway protein

Products: NA

Alternate protein names: Aminohydrolase

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MRISPSEAAMKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAEDYSIAQMAAELH
QALVAAGIKRYAVVGHALGALVGMQLALDYPASVTMLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPA
DWMAARAPRLEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMV
MRYGGHACNVTHPETFNALLLNGLASLLHHREAACKELL

Sequences:

>Translated_279_residues
MRISPSEAAMKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAEDYSIAQMAAELH
QALVAAGIKRYAVVGHALGALVGMQLALDYPASVTMLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPA
DWMAARAPRLEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMV
MRYGGHACNVTHPETFNALLLNGLASLLHHREAACKELL
>Mature_279_residues
MRISPSEAAMKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAEDYSIAQMAAELH
QALVAAGIKRYAVVGHALGALVGMQLALDYPASVTMLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPA
DWMAARAPRLEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMV
MRYGGHACNVTHPETFNALLLNGLASLLHHREAACKELL

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family

Homologues:

Organism=Escherichia coli, GI1787244, Length=265, Percent_Identity=96.6037735849057, Blast_Score=504, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTD_ECOLU (B7N3G5)

Other databases:

- EMBL:   CU928163
- RefSeq:   YP_002411945.1
- ProteinModelPortal:   B7N3G5
- EnsemblBacteria:   EBESCT00000116915
- GeneID:   7157826
- GenomeReviews:   CU928163_GR
- GeneTree:   EBGT00050000009729
- HOGENOM:   HBG748003
- OMA:   CIVQEMA
- ProtClustDB:   CLSK879922
- BioCyc:   ECOL585056:ECUMN_1192-MONOMER
- HAMAP:   MF_00832
- InterPro:   IPR000073
- InterPro:   IPR019913
- PRINTS:   PR00111
- TIGRFAMs:   TIGR03611

Pfam domain/function: PF00561 Abhydrolase_1

EC number: NA

Molecular weight: Translated: 30364; Mature: 30364

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISPSEAAMKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTG
CCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCC
NNPDTLAEDYSIAQMAAELHQALVAAGIKRYAVVGHALGALVGMQLALDYPASVTMLVSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
NGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPRLEAEDALALAH
CCEEEECHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHCCCCCCCHHHEEHHH
FQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMV
HCCHHHHHHHHHHHHHCCCHHCHHHHCCCEEEEECCCCEECCCCCCCHHHHCCCCHHHHH
MRYGGHACNVTHPETFNALLLNGLASLLHHREAACKELL
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRISPSEAAMKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTG
CCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCC
NNPDTLAEDYSIAQMAAELHQALVAAGIKRYAVVGHALGALVGMQLALDYPASVTMLVSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
NGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPRLEAEDALALAH
CCEEEECHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHCCCCCCCHHHEEHHH
FQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMV
HCCHHHHHHHHHHHHHCCCHHCHHHHCCCEEEEECCCCEECCCCCCCHHHHCCCCHHHHH
MRYGGHACNVTHPETFNALLLNGLASLLHHREAACKELL
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA