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The map label for this gene is pncB [H]

Identifier: 218704359

GI number: 218704359

Start: 1176755

End: 1177957

Strand: Reverse

Name: pncB [H]

Synonym: ECUMN_1125

Alternate gene names: 218704359

Gene position: 1177957-1176755 (Counterclockwise)

Preceding gene: 218704363

Following gene: 218704358

Centisome position: 22.64

GC content: 51.29

Gene sequence:

>1203_bases
ATGACACAATTCGCTTCTCCTGTTCTGCACTCGTTGCTGGATACAGATGCTTATAAGTTGCATATGCAGCAAGCCGTGTT
TCATCACTATTACGATGTGCATGTCGCGGCGGAGTTTCGTTGCCGAGGTGACGATCTGCTGGGTATTTATGCCGATGCTA
TTCGTGAACAGGTTCAGGCGATGCAGCACCTGCGCCTGCAGGATGATGAATATCAATGGCTCTCAGCCCTGCCTTTCTTT
AAGGCCGACTATCTTAACTGGCTACGCGAGTTCCGCTTTAACCCGGAACAAGTCACCGTGTCCAACGATAATGGCAAGCT
GGATATTCGTTTAAGCGGCCCGTGGCGTGAAGTCATCCTCTGGGAAGTTCCTTTGCTGGCGGTTATCAGTGAAATGGTAC
ATCGCTATCGCTCACCGCAGGCCGACGTTGCGCAAGCCCTCGACACGCTGGAAAGCAAATTAGCCGATTTCTCGGCATTA
ACCGCCGGTCTTGATATGTCGCGCTTCCATCTGATGGATTTTGGCACCCGCCGACGTTTTTCTCGTGAAGTACAAGAAAC
CATCGTGAAACGTCTGCAACAGGAATCCTGGTTCGTGGGCACCAGCAACTACGATCTGGCGCGTCGGCTTTCCCTCACGC
CAATGGGAACACAAGCACACGAATGGTTCCAGGCGCATCAGCAAATCAGTCCGGATCTTGCCAACAGCCAGCGAGCTGCA
CTTGCTGCCTGGCTGGAAGAATATCCCGACCAACTTGGCATTGCATTAACCGACTGCATCACTATGGATGCTTTCCTGCG
TGATTTCGGTGTCGAGTTCGCCAGTCGCTATCAGGGCCTGCGTCATGACTCCGGCGACCCGGTTGAATGGGGTGAAAAAG
CCATTGCACATTATGAGAAGCTGGGAATTGATCCACAGAGTAAAACGCTGGTTTTCTCTGACAATCTGGATTTACGCAAA
GCGGTTGAGCTATACCGCCACTTCTCTTCCCGCGTGCAATTAAGTTTTGGTATTGGGACTCGCCTGACCTGCGATATCCC
CCAGGTAAAACCCCTGAATATTGTCATTAAGTTGGTAGAGTGTAACGGTAAACCGGTGGCGAAACTTTCTGACAGCCCTG
GCAAAACTATCTGCCACGATAAAGCGTTTGTTCGGGCGCTGCGCAAAGCGTTCGACCTTCCGCATATTAAAAAAGCCAGT
TAA

Upstream 100 bases:

>100_bases
GATCGGGGTTCAATAAATTGCGAAACAAGGTATACTCCAGCAGTTCCTGAAGATGTTTATTGTACTAAACGCTCCTGTAC
GAGGACGCTACTGCGCACCT

Downstream 100 bases:

>100_bases
TATCATCAGGGAGCTAATCGGCTCCCTTTTTTTACCTTTAATTCCGAAATCTTTCGCTGCATTTGCGAATTCTGCTTGTC
TGATTGCAGATGCCAGGTAA

Product: nicotinate phosphoribosyltransferase

Products: NA

Alternate protein names: NAPRTase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGDDLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFF
KADYLNWLREFRFNPEQVTVSNDNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQAHEWFQAHQQISPDLANSQRAA
LAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASRYQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRK
AVELYRHFSSRVQLSFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFDLPHIKKAS

Sequences:

>Translated_400_residues
MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGDDLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFF
KADYLNWLREFRFNPEQVTVSNDNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQAHEWFQAHQQISPDLANSQRAA
LAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASRYQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRK
AVELYRHFSSRVQLSFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFDLPHIKKAS
>Mature_399_residues
TQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGDDLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFK
ADYLNWLREFRFNPEQVTVSNDNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSALT
AGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQAHEWFQAHQQISPDLANSQRAAL
AAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASRYQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKA
VELYRHFSSRVQLSFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFDLPHIKKAS

Specific function: NAD biosynthesis; nicotinamide to NaMN; second step. [C]

COG id: COG1488

COG function: function code H; Nicotinic acid phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAPRTase family [H]

Homologues:

Organism=Escherichia coli, GI1787162, Length=400, Percent_Identity=99.75, Blast_Score=829, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324783, Length=419, Percent_Identity=33.6515513126492, Blast_Score=214, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006406
- InterPro:   IPR015977
- InterPro:   IPR007229
- InterPro:   IPR002638 [H]

Pfam domain/function: PF04095 NAPRTase [H]

EC number: =2.4.2.11 [H]

Molecular weight: Translated: 45870; Mature: 45738

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGDDLLGIYADAIREQVQA
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHH
MQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSNDNGKLDIRLSGPWREVIL
HHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCHHHHHH
WEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSALTAGLDMSRFHLMDFGTRRRF
HHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQAHEWFQAHQQISPDLANSQRAA
HHHHHHHHHHHHHHCCEEECCCCCCHHHHEECCCCCCHHHHHHHHHHHCCCCCCCCHHHH
LAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASRYQGLRHDSGDPVEWGEKAIAHYEK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQLSFGIGTRLTCDIPQVKPLNIVIKLVE
HCCCCCCCEEEEECCCCHHHHHHHHHHHHHCEEEEECCCCEEEECCCCCCCCEEEEEEEE
CNGKPVAKLSDSPGKTICHDKAFVRALRKAFDLPHIKKAS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGDDLLGIYADAIREQVQA
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHH
MQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSNDNGKLDIRLSGPWREVIL
HHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCHHHHHH
WEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSALTAGLDMSRFHLMDFGTRRRF
HHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQAHEWFQAHQQISPDLANSQRAA
HHHHHHHHHHHHHHCCEEECCCCCCHHHHEECCCCCCHHHHHHHHHHHCCCCCCCCHHHH
LAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASRYQGLRHDSGDPVEWGEKAIAHYEK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQLSFGIGTRLTCDIPQVKPLNIVIKLVE
HCCCCCCCEEEEECCCCHHHHHHHHHHHHHCEEEEECCCCEEEECCCCCCCCEEEEEEEE
CNGKPVAKLSDSPGKTICHDKAFVRALRKAFDLPHIKKAS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA