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The map label for this gene is acrA [H]

Identifier: 218703746

GI number: 218703746

Start: 543142

End: 544335

Strand: Reverse

Name: acrA [H]

Synonym: ECUMN_0502

Alternate gene names: 218703746

Gene position: 544335-543142 (Counterclockwise)

Preceding gene: 218703749

Following gene: 218703745

Centisome position: 10.46

GC content: 53.85

Gene sequence:

>1194_bases
ATGAACAAAAACAGAGGGTTTACGCCTCTGGCGGTCGTTCTGATGCTCTCAGGCAGCTTAGCCCTAACAGGATGTGACGA
CAAACAGGCCCAACAAGGTGGCCAGCAGATGCCCGCCGTTGGCGTAGTAACAGTCAAAACTGAACCTCTGCAGATCACAA
CCGAGCTTCCGGGTCGCACCAGTGCCTACCGGATCGCAGAAGTTCGTCCTCAAGTTAGCGGGATTATCCTGAAGCGTAAT
TTCAAAGAAGGTAGCGACATCGAAGCAGGTGTCTCTCTCTATCAGATTGATCCTGCGACCTATCAGGCGGCATACGACAG
CGCGAAAGGCGATCTGGCGAAAGCCCAGGCTGCAGCCAATATCGCGCAATTGACGGTGAATCGTTATCAGAAATTGCTCG
GTACTCAGTACATCAGTAAGCAAGAGTACGATCAGGCTCTGGCTGATGCGCAACAGGCAAATGCTGCGGTAACTGCGGCG
AAAGCTGCCGTTGAAACTGCGCGGATCAATCTGGCTTACACCAAAGTTACCTCTCCGATTAGCGGTCGCATTGGTAAGTC
GAACGTGACGGAAGGCGCATTGGTACAGAACGGTCAGGCGACTGCGCTGGCAACCGTGCAGCAACTTGATCCGATCTACG
TTGATGTGACCCAGTCCAGCAACGACTTCCTGCGCCTGAAACAGGAACTGGCAAATGGCACGCTGAAACAAGAGAACGGC
AAAGCCAAAGTGTCGCTGATCACCAGTGACGGCATTAAGTTCCCGCAGGACGGTACGCTGGAATTCTCTGACGTTACCGT
TGATCAGACCACCGGGTCTATCACCCTACGCGCTATCTTCCCGAACCCGGATCACACTCTGCTGCCGGGTATGTTCGTAC
GTGCACGTCTGGAAGAAGGGCTTAATCCAAACGCTATTTTAGTCCCGCAACAGGGCGTAACCCGTACGCCGCGTGGCGAT
GCCACCGTACTGGTGGTTGGCGCGGATGACAAAGTGGAAACCCGTCCGATCGTTGCAAGCCAGGCTATTGGCGATAAGTG
GCTGGTGACAGAAGGTCTGAAAGCAGGCGATCGCGTAGTAATAAGTGGGCTGCAGAAAGTGCGTCCTGGTGTCCAGGTAA
AAGCACAAGAAGTTACCGCTGATAATAACCAGCAAGCCGCAAGCGGTGCTCAGCCTGAACAGTCCAAGTCTTAA

Upstream 100 bases:

>100_bases
GAATGTATGTACCATAGCACGACGATAATATAAACGCAGCAATGGGTTTATTAACTTTTGACCATTGACCAATTTGAAAT
CGGACACTCGAGGTTTACAT

Downstream 100 bases:

>100_bases
CTTAAACAGGAGCCGTTAAGACATGCCTAATTTCTTTATCGATCGCCCGATTTTTGCGTGGGTGATCGCCATTATCATCA
TGTTGGCAGGGGGGCTGGCG

Product: multidrug efflux system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVTVKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRN
FKEGSDIEAGVSLYQIDPATYQAAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGD
ATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQPEQSKS

Sequences:

>Translated_397_residues
MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVTVKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRN
FKEGSDIEAGVSLYQIDPATYQAAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGD
ATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQPEQSKS
>Mature_397_residues
MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVTVKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRN
FKEGSDIEAGVSLYQIDPATYQAAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGD
ATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQPEQSKS

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=397, Percent_Identity=99.7481108312343, Blast_Score=795, Evalue=0.0,
Organism=Escherichia coli, GI1789665, Length=375, Percent_Identity=69.3333333333333, Blast_Score=513, Evalue=1e-147,
Organism=Escherichia coli, GI1789929, Length=382, Percent_Identity=54.4502617801047, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI87082036, Length=321, Percent_Identity=30.2180685358255, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081951, Length=117, Percent_Identity=35.8974358974359, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789637, Length=150, Percent_Identity=30.6666666666667, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42167; Mature: 42167

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVTVKTEPLQITTELPGRT
CCCCCCCCHHHHHHHHCCCEEEECCCHHHHHCCCCCCCEEEEEEEECCCEEEEECCCCCC
SAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQAAYDSAKGDLAKAQAAAN
CEEEEEECCCCCEEEEEEECCCCCCCCHHCEEEEEECCHHHHHHHHCCCCHHHHHHHHHH
IAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAAKAAVETARINLAYTKVTSPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEEEEECCCC
SGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
CCCCCCCCCCCCCEEECCCCCEEHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCEECCCC
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEG
CEEEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCC
LNPNAILVPQQGVTRTPRGDATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVV
CCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEEHHCCCCCEEEECCCCCCCHHH
ISGLQKVRPGVQVKAQEVTADNNQQAASGAQPEQSKS
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVTVKTEPLQITTELPGRT
CCCCCCCCHHHHHHHHCCCEEEECCCHHHHHCCCCCCCEEEEEEEECCCEEEEECCCCCC
SAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQAAYDSAKGDLAKAQAAAN
CEEEEEECCCCCEEEEEEECCCCCCCCHHCEEEEEECCHHHHHHHHCCCCHHHHHHHHHH
IAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAAKAAVETARINLAYTKVTSPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEEEEECCCC
SGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
CCCCCCCCCCCCCEEECCCCCEEHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCEECCCC
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEG
CEEEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCC
LNPNAILVPQQGVTRTPRGDATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVV
CCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEEHHCCCCCEEEECCCCCCCHHH
ISGLQKVRPGVQVKAQEVTADNNQQAASGAQPEQSKS
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]