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The map label for this gene is sbcD [H]

Identifier: 218703679

GI number: 218703679

Start: 471008

End: 472210

Strand: Reverse

Name: sbcD [H]

Synonym: ECUMN_0435

Alternate gene names: 218703679

Gene position: 472210-471008 (Counterclockwise)

Preceding gene: 218703686

Following gene: 218703678

Centisome position: 9.08

GC content: 53.03

Gene sequence:

>1203_bases
ATGCGCATCCTTCACACCTCAGACTGGCATCTCGGCCAGAACTTCTACAGTAAAAGCCGCGAAGCTGAACATCAGGCTTT
TCTTGACTGGCTGCTGGAGACAGCACAAACCCATCAGGTGGATGCGATTATTGTTGCCGGTGATGTTTTCGATACCGGCT
CGCCCCCCAGTTACGCCCGCACGTTATACAACCGTTTTGTTGTCAATTTACAGCAAACTGGCTGTCATCTGGTGGTACTG
GCAGGAAACCATGACTCGGTCGCCACGCTGAATGAATCGCGCGATATCATGGCGTTCCTCAATACGACCGTGGTCGCCAG
CGCCGGACATGCGCCGCAAATCTTGCCTCGTCGCGACGGGACGCCAGGCGCAGTGCTGTGCCCTATTCCGTTTTTACGTC
CGCGTGACATTATTACCAGCCAGGCGGGGCTTAACGGTATTGAAAAACAGCAGCATTTGCTGGCGGCTATTACTGATTAT
TATCAACAACATTATGCCGATGCCTGCAAACTGCGCGGCGATCAGCCTCTGCCCATCATCGCCACGGGACATTTAACGAC
CGTGGGTGCCAGTAAAAGTGACGCCGTGCGTGACATTTATATTGGCACGCTGGACGCGTTTCCGGCACAAAACTTTCCAC
CAGCCGACTACATCGCGCTCGGGCATATTCACCGCGCGCAGATTATTGGCGGCATGGAACATGTTCGCTATTGCGGTTCC
CCCATACCACTGAGTTTTGATGAATGCGGTAAGAGTAAATATGTCCATCTGGTGACATTTTCAAACGGCAAATTAGAGAG
CGTGGAAAACCTGAACGTACCGGTAACGCAACCCATGGCGGTGCTGAAAGGCGATCTGGCGTCGATTACCGAACAGCTGG
AACAGTGGCGCGATGTATCACAGGAGCCACCTGTCTGGCTGGATATCGAAATCACTACTGATGAGTATCTGCATGATATT
CAGCGCAAAATCCAGGCATTAACCGAATCATTGCCTGTCGAAGTATTGCTGGTACGCCGGAGTCGTGAACAGCGCGAGCG
AGTGTTAGCCAGCCAACAGCGTGAAACCCTCAGCGAACTCAGCGTCGAAGAGGTGTTCAATCGCCGTCTGGCACTGGAAG
AACTGGATGAATCGCAGCAGCAACGTCTGCAGCATCTTTTCACCACGACGTTGCATACCCTCGCCGGAGAACACGAAGCA
TGA

Upstream 100 bases:

>100_bases
CACATATCTTCAGGTTATTGATTTCCGTGGCGCAGAAAAAAGCAAATGGCACATCTGTTTGGGTATAATCGCGCCCATGC
TTTTTCGCCAGGGAACCGTT

Downstream 100 bases:

>100_bases
AAATTCTCAGCCTGCGCCTGAAAAACCTGAACTCATTAAAAGGCGAATGGAAGATTGATTTCACCCGCGAGCCGTTCGCC
AGCAACGGGCTGTTTGCCAT

Product: exonuclease subunit SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVL
AGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDI
QRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLHTLAGEHEA

Sequences:

>Translated_400_residues
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVL
AGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDI
QRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLHTLAGEHEA
>Mature_400_residues
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVL
AGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDI
QRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLHTLAGEHEA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=400, Percent_Identity=99.75, Blast_Score=825, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 44772; Mature: 44772

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYAR
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHH
TLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDG
HHHHHHHHEEECCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHEECCCCCCCCCCCCCC
TPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDYYQQHYADACKLRGDQPLPII
CCCEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
ATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
EECCEEEECCCCCCCHHEEEEEECCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCC
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITEQLEQWRDVS
CCCCCHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHCCC
QEPPVWLDIEITTDEYLHDIQRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSEL
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
SVEEVFNRRLALEELDESQQQRLQHLFTTTLHTLAGEHEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYAR
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHH
TLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDG
HHHHHHHHEEECCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHEECCCCCCCCCCCCCC
TPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDYYQQHYADACKLRGDQPLPII
CCCEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
ATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
EECCEEEECCCCCCCHHEEEEEECCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCC
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITEQLEQWRDVS
CCCCCHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHCCC
QEPPVWLDIEITTDEYLHDIQRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSEL
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
SVEEVFNRRLALEELDESQQQRLQHLFTTTLHTLAGEHEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]