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The map label for this gene is 218703473

Identifier: 218703473

GI number: 218703473

Start: 252650

End: 253996

Strand: Reverse

Name: 218703473

Synonym: ECUMN_0221

Alternate gene names: NA

Gene position: 253996-252650 (Counterclockwise)

Preceding gene: 218703474

Following gene: 218703472

Centisome position: 4.88

GC content: 58.05

Gene sequence:

>1347_bases
ATGGCAACAATGAAAAACAAGGTGATCTGGCAGGAAGGGCTGTTTGCCCTGCCGCAGCATTTTCAGCAACAGCAGCGTCA
TTGCGAGTACCTGCTGAACCGTCGTCTGGATGCACTGGGTGATTTTTTCTGGGGCTTTACGGAACTCTCCATCAATACCG
AACTGCTTGCCCAGGGCAAAATCATGATTGACCGTGCGGCGGGCTGTATGCCTGACGGCACCGTATTCAGCATTCCCGAC
CAGGATCTGCTGCCGGAACCTTTCCAGCCGGGAACCCTTTCATCGAAAGAGAGCCACAATATTTATCTGGCGCTGCCTGT
AATCAGCGACGTGATCAATGAAATCCAGGGGTTGCACAGCGCCGGTCAGGGTACGGAGCGTTACCGCCTCACCCATACCC
GCGTCCGTGATTTTCACACTGACGAAGGCGACGAACAGCCGGTCGGTCTGGGGCAGTTGATCCCCAGGATAGTCAGCGGC
GCAGATGATCTCAGCGCGATGGTGACCCTCCCCCTGTGCCGTATCCTCAATAAAAATGCTACCGGCGCACTGGTGCTGGA
CAACACCTTTATTCCCACCATTCAGGCCGTCCGTGTCAGCGGGTTGCTCGGGGCATTCTCCGGCGAGGTGCAGGGGCTGC
TTGCCACCCGCGCCGCCGACCTGGCCGGGCGCATCGGTTCGCCTGAACAGAGCGGGATCGCCGATGTGGCCGAATTTATG
ATGTTGCAGATGCTTAACCGCCATCAAATGCAGTTCACGCACCGTTCACAACTGCACACGCTGCATCCGGAAGCCTTCTA
CCAAGATCTGGTGGGGCTGCTGGGAGAACTGATGACCTTTACCGAAGGTAATCGCCTGCCCTGCACCGTGGAGCCGTATA
ATCACCGGGATCTTACCGCGACCTTCACCAGAACCGTGATCCCGGAACTGCGGCGCGCACTCAATACCGTACTGGTGCCG
CGTGCGCAGAATCTGCCGCTGTTCTTTTCAGAGGGGACCTGGATCGCCACCATCAGCGATCCCACACTGTTGCAGTCCTG
CAAGCTGGTACTGGCAGTGCGCGCCAGGATGCCCTACGACCAGGTACAACGCCAGTTCATCCAGCAGTCAAAAGTGGCCG
CTACCGACAAAATCCGCAGCGTGGTCAGCGTGCAGGTGCCGGGTATTCCGCTGCACACTCTTACCGCTGCGCCGCGTCAG
TTGCCCTGGCATGAAGGTTATGTTTACTTCGAACTGGAAAAAGGCACCCCAGCCTGGCAGGACGTGATAAAAGCGGGCGC
GCTGGCGCTGCATATCTCCGGCACCTTCCCCGATCTGAACCTTCAGCTCTGGGCGATAAGGGGATAA

Upstream 100 bases:

>100_bases
GGAAACAAATTGAGCCGGTAGAAAGTATCGGCCACCACTATCGCCTGCTGGTGCATATCCGCGCCAGTGCGATTGAGATG
AAAAAAGAGGAAAACTGACG

Downstream 100 bases:

>100_bases
GTGAATGAGCGATATCAGCGATATGGGTGAACCGTCGGTTTTTCCCATGGAGCCGGAGAGCGGAACATCAGGACGTCAGT
ACCGTCTCGCCCTTCGCGGC

Product: hypothetical protein

Products: NA

Alternate protein names: Type VI Secretion Protein Family; Protein ImpJ/VasE; Conserved Protein; Replication/Virulence Associated Protein; Type VI Secretion System Protein EvpM; Protein ImpJ; Type VI Secretion System Protein ImpJ; Type VI Secretion; Type VI Secretion System Core Protein; T6ss M; Type VI Secretion Protein VasE; Temperature-Dependent Protein Secretion ImpJ

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MATMKNKVIWQEGLFALPQHFQQQQRHCEYLLNRRLDALGDFFWGFTELSINTELLAQGKIMIDRAAGCMPDGTVFSIPD
QDLLPEPFQPGTLSSKESHNIYLALPVISDVINEIQGLHSAGQGTERYRLTHTRVRDFHTDEGDEQPVGLGQLIPRIVSG
ADDLSAMVTLPLCRILNKNATGALVLDNTFIPTIQAVRVSGLLGAFSGEVQGLLATRAADLAGRIGSPEQSGIADVAEFM
MLQMLNRHQMQFTHRSQLHTLHPEAFYQDLVGLLGELMTFTEGNRLPCTVEPYNHRDLTATFTRTVIPELRRALNTVLVP
RAQNLPLFFSEGTWIATISDPTLLQSCKLVLAVRARMPYDQVQRQFIQQSKVAATDKIRSVVSVQVPGIPLHTLTAAPRQ
LPWHEGYVYFELEKGTPAWQDVIKAGALALHISGTFPDLNLQLWAIRG

Sequences:

>Translated_448_residues
MATMKNKVIWQEGLFALPQHFQQQQRHCEYLLNRRLDALGDFFWGFTELSINTELLAQGKIMIDRAAGCMPDGTVFSIPD
QDLLPEPFQPGTLSSKESHNIYLALPVISDVINEIQGLHSAGQGTERYRLTHTRVRDFHTDEGDEQPVGLGQLIPRIVSG
ADDLSAMVTLPLCRILNKNATGALVLDNTFIPTIQAVRVSGLLGAFSGEVQGLLATRAADLAGRIGSPEQSGIADVAEFM
MLQMLNRHQMQFTHRSQLHTLHPEAFYQDLVGLLGELMTFTEGNRLPCTVEPYNHRDLTATFTRTVIPELRRALNTVLVP
RAQNLPLFFSEGTWIATISDPTLLQSCKLVLAVRARMPYDQVQRQFIQQSKVAATDKIRSVVSVQVPGIPLHTLTAAPRQ
LPWHEGYVYFELEKGTPAWQDVIKAGALALHISGTFPDLNLQLWAIRG
>Mature_447_residues
ATMKNKVIWQEGLFALPQHFQQQQRHCEYLLNRRLDALGDFFWGFTELSINTELLAQGKIMIDRAAGCMPDGTVFSIPDQ
DLLPEPFQPGTLSSKESHNIYLALPVISDVINEIQGLHSAGQGTERYRLTHTRVRDFHTDEGDEQPVGLGQLIPRIVSGA
DDLSAMVTLPLCRILNKNATGALVLDNTFIPTIQAVRVSGLLGAFSGEVQGLLATRAADLAGRIGSPEQSGIADVAEFMM
LQMLNRHQMQFTHRSQLHTLHPEAFYQDLVGLLGELMTFTEGNRLPCTVEPYNHRDLTATFTRTVIPELRRALNTVLVPR
AQNLPLFFSEGTWIATISDPTLLQSCKLVLAVRARMPYDQVQRQFIQQSKVAATDKIRSVVSVQVPGIPLHTLTAAPRQL
PWHEGYVYFELEKGTPAWQDVIKAGALALHISGTFPDLNLQLWAIRG

Specific function: Unknown

COG id: COG3522

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 49861; Mature: 49730

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKNKVIWQEGLFALPQHFQQQQRHCEYLLNRRLDALGDFFWGFTELSINTELLAQGK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHCCCC
IMIDRAAGCMPDGTVFSIPDQDLLPEPFQPGTLSSKESHNIYLALPVISDVINEIQGLHS
EEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHH
AGQGTERYRLTHTRVRDFHTDEGDEQPVGLGQLIPRIVSGADDLSAMVTLPLCRILNKNA
CCCCCCCEEEEHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
TGALVLDNTFIPTIQAVRVSGLLGAFSGEVQGLLATRAADLAGRIGSPEQSGIADVAEFM
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
MLQMLNRHQMQFTHRSQLHTLHPEAFYQDLVGLLGELMTFTEGNRLPCTVEPYNHRDLTA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHH
TFTRTVIPELRRALNTVLVPRAQNLPLFFSEGTWIATISDPTLLQSCKLVLAVRARMPYD
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHCCCHH
QVQRQFIQQSKVAATDKIRSVVSVQVPGIPLHTLTAAPRQLPWHEGYVYFELEKGTPAWQ
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHCCCCCCCCCCCCEEEEEECCCCCHHH
DVIKAGALALHISGTFPDLNLQLWAIRG
HHHHHCEEEEEEECCCCCCCEEEEEECC
>Mature Secondary Structure 
ATMKNKVIWQEGLFALPQHFQQQQRHCEYLLNRRLDALGDFFWGFTELSINTELLAQGK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHCCCC
IMIDRAAGCMPDGTVFSIPDQDLLPEPFQPGTLSSKESHNIYLALPVISDVINEIQGLHS
EEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHH
AGQGTERYRLTHTRVRDFHTDEGDEQPVGLGQLIPRIVSGADDLSAMVTLPLCRILNKNA
CCCCCCCEEEEHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
TGALVLDNTFIPTIQAVRVSGLLGAFSGEVQGLLATRAADLAGRIGSPEQSGIADVAEFM
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
MLQMLNRHQMQFTHRSQLHTLHPEAFYQDLVGLLGELMTFTEGNRLPCTVEPYNHRDLTA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHH
TFTRTVIPELRRALNTVLVPRAQNLPLFFSEGTWIATISDPTLLQSCKLVLAVRARMPYD
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHCCCHH
QVQRQFIQQSKVAATDKIRSVVSVQVPGIPLHTLTAAPRQLPWHEGYVYFELEKGTPAWQ
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHCCCCCCCCCCCCEEEEEECCCCCHHH
DVIKAGALALHISGTFPDLNLQLWAIRG
HHHHHCEEEEEEECCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA