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The map label for this gene is mltD

Identifier: 218703460

GI number: 218703460

Start: 235668

End: 237026

Strand: Reverse

Name: mltD

Synonym: ECUMN_0208

Alternate gene names: 218703460

Gene position: 237026-235668 (Counterclockwise)

Preceding gene: 218703461

Following gene: 218703457

Centisome position: 4.56

GC content: 52.1

Gene sequence:

>1359_bases
ATGAAGGCAAAAGCGATATTACTCGCCTCTGTCCTGCTCGTGGGTTGCCAGAGTACCGGCAACGTTCAACAGCACGCACA
GAGCCTTTCTGCAGCTGGTCAAGGGGAAGCAGCAAAGTTTACAAGTCAGGCACGATGGATGGACGATGGGACGTCTATCG
CGCCAGATGGTGACTTGTGGGCTTTCATTGGCGACGAGCTAAAGATGGGAATTCCGGAAAATGACCGGATTCGCGAACAG
AAACAGAAATATTTACGCAATAAGAGCTATCTCCACGATGTAACTTTACGGGCAGAGCCGTATATGTACTGGATAGCCGG
GCAAGTTAAAAAACGTAACATGCCTATGGAACTGGTACTACTACCCATAGTGGAGAGCGCTTTTGATCCTCACGCAACGT
CTGGCGCCAATGCCGCAGGCATCTGGCAGATCATTCCGAGCACGGGGCGCAATTATGGTTTGAAACAGACCCGCAATTAT
GACGCGCGTCGCGATGTTGTTGCTTCAACAACTGCCGCGCTGAACATGATGCAGCGTCTGAACAAGATGTTTGACGGCGA
CTGGCTTCTGACCGTAGCGGCTTATAACAGCGGCGAAGGTCGAGTCATGAAGGCAATTAAAACGAACAAAGCGCGTGGGA
AATCCACGGACTTCTGGTCGTTACCGTTGCCGCAGGAAACGAAGCAGTACGTGCCTAAAATGCTGGCATTGAGTGATATT
CTCAAAAACAGCAAGCGTTATGGCGTACGTCTGCCAACGACCGATGAAAGCCGTGCTCTGGCGCGTGTGCACCTGAGCAG
CCCAGTTGAAATGGCGAAGGTTGCAGATATGGCGGGGATTTCCGTCAGCAAGCTGAAGACATTCAACGCTGGCGTGAAAG
GCTCCACGCTGGGCGCAAGTGGTCCGCAGTACGTGATGGTGCCAAAGAAGCATGCAGATCAACTGCGTGAATCTCTGGCT
TCGGGCGAAATTGCTGCTGTACAGTCGACGCTGGTTGCCGACAATACGCCGCTTAACAGCCGTGTTTACACCGTACGCTC
TGGCGACACGCTTTCAAGTATCGCTTCACGTCTCGGCGTTAGCACCAAAGATTTGCAGCAGTGGAACAAACTGCGCGGAT
CTAAGCTGAAGCCAGGCCAAAGCTTGACGATTGGCGCAGGTAGTAGCGCACAGCGGTTGGCAAACAACAGCGATAGCATT
ACGTATCGTGTGCGCAAAGGCGATTCGCTTTCAAGCATTGCTAAACGCCACGGCGTGAACATCAAAGATGTGATGCGCTG
GAACAGCGATACTGCGAATCTGCAACCAGGCGATAAGCTGACGTTGTTTGTGAAAAACAACAACATGCCAGATTCCTGA

Upstream 100 bases:

>100_bases
GGTTAAGGTCAAAGAAAGATAGGTTCTGATAAAACTTTCTTGTCATCGGCTCCGTTCGCCGTTATGATCGGTCGTCTTTT
AAGCAACTATTGACACACAC

Downstream 100 bases:

>100_bases
CAAACCAGATAATAAAAAGGCACCGATTCCCCCGGTGCCTTTTTTATTTATGCTGCTTTATGTGCTTCTACCATGATGAT
ATCACTGGTGAAAGAGCCGT

Product: membrane-bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQARWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQ
KQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDFWSLPLPQETKQYVPKMLALSDI
LKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVADMAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLA
SGEIAAVQSTLVADNTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQRLANNSDSI
TYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS

Sequences:

>Translated_452_residues
MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQARWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQ
KQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDFWSLPLPQETKQYVPKMLALSDI
LKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVADMAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLA
SGEIAAVQSTLVADNTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQRLANNSDSI
TYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS
>Mature_452_residues
MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQARWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQ
KQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDFWSLPLPQETKQYVPKMLALSDI
LKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVADMAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLA
SGEIAAVQSTLVADNTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQRLANNSDSI
TYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats

Homologues:

Organism=Escherichia coli, GI1786405, Length=452, Percent_Identity=100, Blast_Score=928, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): MLTD_ECOL6 (P0AEZ8)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_752194.1
- ProteinModelPortal:   P0AEZ8
- EnsemblBacteria:   EBESCT00000043922
- GeneID:   1036039
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c0248
- GeneTree:   EBGT00050000011852
- HOGENOM:   HBG519241
- OMA:   YAIAAYN
- ProtClustDB:   PRK10783
- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189
- SMART:   SM00257

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT

EC number: 3.2.1.- [C]

Molecular weight: Translated: 49418; Mature: 49418

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00922 TRANSGLYCOSYLASE; PS00013 PROKAR_LIPOPROTEIN

Important sites: ACT_SITE 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQARWMDDGTSIAPDGDLW
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCEE
AFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVL
EEECCHHCCCCCCCHHHHHHHHHHHHCCCHHEEEEEECCCEEEEEECHHHCCCCCCEEEE
LPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNYDARRDVVASTTAALNMMQRL
HHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDFWSLPLPQETKQYVPKMLALSDI
HHHCCCCEEEEEEEEECCCCEEEEEEHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHH
LKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVADMAGISVSKLKTFNAGVKGSTLGAS
HHCCCCCCEECCCCCCCHHEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCC
GPQYVMVPKKHADQLRESLASGEIAAVQSTLVADNTPLNSRVYTVRSGDTLSSIASRLGV
CCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCC
STKDLQQWNKLRGSKLKPGQSLTIGAGSSAQRLANNSDSITYRVRKGDSLSSIAKRHGVN
CHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCC
IKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS
HHHHHHCCCCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQARWMDDGTSIAPDGDLW
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCEE
AFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVL
EEECCHHCCCCCCCHHHHHHHHHHHHCCCHHEEEEEECCCEEEEEECHHHCCCCCCEEEE
LPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNYDARRDVVASTTAALNMMQRL
HHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDFWSLPLPQETKQYVPKMLALSDI
HHHCCCCEEEEEEEEECCCCEEEEEEHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHH
LKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVADMAGISVSKLKTFNAGVKGSTLGAS
HHCCCCCCEECCCCCCCHHEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCC
GPQYVMVPKKHADQLRESLASGEIAAVQSTLVADNTPLNSRVYTVRSGDTLSSIASRLGV
CCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCC
STKDLQQWNKLRGSKLKPGQSLTIGAGSSAQRLANNSDSITYRVRKGDSLSSIAKRHGVN
CHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCC
IKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS
HHHHHHCCCCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157