Click here to switch to the map view.

The map label for this gene is ligT [H]

Identifier: 218703403

GI number: 218703403

Start: 165486

End: 166016

Strand: Reverse

Name: ligT [H]

Synonym: ECUMN_0144

Alternate gene names: 218703403

Gene position: 166016-165486 (Counterclockwise)

Preceding gene: 218703410

Following gene: 218703402

Centisome position: 3.19

GC content: 56.31

Gene sequence:

>531_bases
ATGTCTGAACCGCAACGGCTGTTCTTTGCTATCGACTTACCTGCAGAAATCCGCGAACAGATTATCCACTGGCGCGCCAC
ACACTTCCCACCTGACGCGGGACGTCCGGTCGCCGCCGATAATTTGCATCTGACTCTGGCATTTTTAGGCGAAGTGAGCG
CCGAGAAAGAGAAGGCGCTTTCTCTTTTAGCCGGGCGGATTCGTCAACCGGGTTTCACACTCACGCTTGATGACGCCGGT
CAATGGCTGCGTTCGCGTGTGGTGTGGTTAGGGATGCGTCAGCCGCCACGCGGCTTAATCCAGCTGGCGAATATGCTCCG
TTCACAGGCTGCCCACAGCGGTTGTTTTCAAAGCAATCGTCCGTTTCATCCACATATTACCTTATTGCGCGACGCCAGCG
AGGCGGTGACAATCCCGCCGCCAGGTTTTAACTGGTCGTATACGGTGACGGAGTTCACCCTTTACGCCTCCTCGTTTGCG
CGTGGACGCACACGCTACACGCCGCTAAAACGCTGGGCGCTAACGCAATAA

Upstream 100 bases:

>100_bases
GACGGCAGCAACGGGCAACGACGACACAAAACGCTCCAGAGGGTTAACATTCTTCGCGCCACATTGTAGCATCGCGGTAA
TTCATAACCGAGTGCCTCAC

Downstream 100 bases:

>100_bases
CAAGGATTGTCGTAATGGAATTTTCTCCCCCTCTACAGCGCGCGACGCTAATTCAGCGTTACAAACGTTTTTTAGCCGAT
GTGATCACACCCGATGGTCG

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHLTLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAG
QWLRSRVVWLGMRQPPRGLIQLANMLRSQAAHSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
RGRTRYTPLKRWALTQ

Sequences:

>Translated_176_residues
MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHLTLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAG
QWLRSRVVWLGMRQPPRGLIQLANMLRSQAAHSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
RGRTRYTPLKRWALTQ
>Mature_175_residues
SEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHLTLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQ
WLRSRVVWLGMRQPPRGLIQLANMLRSQAAHSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFAR
GRTRYTPLKRWALTQ

Specific function: Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage [H]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=176, Percent_Identity=98.2954545454545, Blast_Score=355, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 19931; Mature: 19800

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHLTLAFLGEVSAEKEKAL
CCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHCCCHHHHHHH
SLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQLANMLRSQAAHSGCFQSNR
HHHHHHHCCCCCEEEECHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
PFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFARGRTRYTPLKRWALTQ
CCCCCEEEEECCCCEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
SEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHLTLAFLGEVSAEKEKAL
CCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHCCCHHHHHHH
SLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQLANMLRSQAAHSGCFQSNR
HHHHHHHCCCCCEEEECHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
PFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFARGRTRYTPLKRWALTQ
CCCCCEEEEECCCCEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 2013578; 8940112 [H]