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The map label for this gene is tbpA [H]

Identifier: 218703328

GI number: 218703328

Start: 78861

End: 79844

Strand: Reverse

Name: tbpA [H]

Synonym: ECUMN_0069

Alternate gene names: 218703328

Gene position: 79844-78861 (Counterclockwise)

Preceding gene: 218703329

Following gene: 218703327

Centisome position: 1.53

GC content: 52.95

Gene sequence:

>984_bases
GTGTTAAAAAAATATCTTCCCCTGCTGTTGCTGTGCACAGCGCCCGTTTTCGCGAAACCTGTTCTGACTGTTTATACCTA
CGATTCCTTCGCCGCTGACTGGGGGCCAGGCCCAGTGGTAAAAAAAGCCTTTGAAGCCGACTGTAATTGCGAACTGAAAC
TGGTAGCGCTGGAAGATGGCGTTTCGCTTCTCAACCGTCTGCGGATGGAAGGCAAAAACAGTAAAGCTGATGTGGTGCTG
GGGCTGGATAACAATCTGTTAGACGCCGCCAGCAAAACCGGACTGTTTGCCAAAAGCGGTGTGGCAGCAGATGCGGTAAA
TGTACCCGGCGGCTGGAATAATGACACTTTCGTACCGTTTGATTACGGCTATTTCGCCTTCGTCTATGACAAGAACAAAC
TGAAAAATCCGCCGCAAAGCCTGAAAGAATTGGTTGAGAGCGATCAAAACTGGCGGGTGATTTATCAGGATCCGCGCACC
AGTACGCCGGGGCTGGGCCTGCTGTTGTGGATGCAAAAAGTCTATGGCGATGACGCCCCACAAGCCTGGCAGAAACTGGC
GAAGAAAACGGTCACGGTCACCAAAGGCTGGAGCGAAGCCTACGGCCTGTTTTTAAAAGGCGAAAGCGATCTGGTACTGA
GTTACACCACCTCTCCGGCTTATCACATTCTCGAAGAGAAGAAAGATAACTACGCCACCGCGAACTTCAGCGAAGGTCAC
TATTTGCAAGTGGAAGTCGCCGCCCGCACCGCTGCCAGCAAGCAGCCAGAGCTGGCGCAAAAATTCCTCCAGTTTATGGT
TTCTCCGGCTTTCCAGAATGCGATCCCAACCGGCAACTGGATGTATCCGGTGGCAAATGTCACGCTGCCTGCCGGGTTTG
AACAATTGACCAAACCGGCAACCACGCTGGAGTTCACGCCAGCCGAAGTGGCGGCACAACGTCAGGCATGGATTAGCGAA
TGGCAACGCGCCGTCAGCCGTTAA

Upstream 100 bases:

>100_bases
TGCGCTGAGAAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCC
ACTCTTTTTTGAGGTGCAAA

Downstream 100 bases:

>100_bases
TTCCCGGCTGGTTAATTCCAGGTGTAAGCGCCGCTACGCTTGTGGTGGCGGTTGCGCTGGCGGCGTTTCTCGCCCTGTGG
TGGAACGCGCCGCAGGGTGA

Product: thiamine transporter substrate binding subunit

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MLKKYLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAFEADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVL
GLDNNLLDAASKTGLFAKSGVAADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTSPAYHILEEKKDNYATANFSEGH
YLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIPTGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISE
WQRAVSR

Sequences:

>Translated_327_residues
MLKKYLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAFEADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVL
GLDNNLLDAASKTGLFAKSGVAADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTSPAYHILEEKKDNYATANFSEGH
YLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIPTGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISE
WQRAVSR
>Mature_327_residues
MLKKYLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAFEADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVL
GLDNNLLDAASKTGLFAKSGVAADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTSPAYHILEEKKDNYATANFSEGH
YLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIPTGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISE
WQRAVSR

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=327, Percent_Identity=99.0825688073395, Blast_Score=664, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36254; Mature: 36254

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKYLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAFEADCNCELKLVALEDG
CCHHHHHHHHHHHCCHHHCCEEEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCC
VSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVAADAVNVPGGWNNDTFVPF
HHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCEECCCCCCCEEECCCCCCCCCEEEE
DYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRTSTPGLGLLLWMQKVYGDDAP
CCCEEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCH
QAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTSPAYHILEEKKDNYATANFSEGH
HHHHHHHHHEEEEECCCHHHCEEEEECCCCEEEEEECCCHHHHHHHCCCCCEECCCCCCC
YLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIPTGNWMYPVANVTLPAGFEQLTKPA
EEEEEEEEHHCCCCCHHHHHHHHHHHHCHHHHHCCCCCCEEEEECCEECCCCHHHHCCCC
TTLEFTPAEVAAQRQAWISEWQRAVSR
CEEEECHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKKYLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAFEADCNCELKLVALEDG
CCHHHHHHHHHHHCCHHHCCEEEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCC
VSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVAADAVNVPGGWNNDTFVPF
HHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCEECCCCCCCEEECCCCCCCCCEEEE
DYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRTSTPGLGLLLWMQKVYGDDAP
CCCEEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCH
QAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTSPAYHILEEKKDNYATANFSEGH
HHHHHHHHHEEEEECCCHHHCEEEEECCCCEEEEEECCCHHHHHHHCCCCCEECCCCCCC
YLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIPTGNWMYPVANVTLPAGFEQLTKPA
EEEEEEEEHHCCCCCHHHHHHHHHHHHCHHHHHCCCCCCEEEEECCEECCCCHHHHCCCC
TTLEFTPAEVAAQRQAWISEWQRAVSR
CEEEECHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]