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The map label for this gene is imp [H]

Identifier: 218703315

GI number: 218703315

Start: 59194

End: 61548

Strand: Reverse

Name: imp [H]

Synonym: ECUMN_0056

Alternate gene names: 218703315

Gene position: 61548-59194 (Counterclockwise)

Preceding gene: 218703318

Following gene: 218703314

Centisome position: 1.18

GC content: 51.3

Gene sequence:

>2355_bases
ATGAAAAAACGTATCCCCACTCTCCTGGCCACCATGATTGCCACCGCCCTTTATAGTCAACAGGGACTGGCAGCCGACCT
CGCCTCACAGTGCATGTTGGGCGTGCCAAGCTATGACCGTCCTCTGGTACAGGGCGATACCAATGACTTACCCGTGACTA
TCAATGCTGACCACGCGAAAGGGGACTACCCGGATGACGCCGTGTTTACTGGCAGCGTGGATATCATGCAGGGTAACAGC
CGTCTGCAGGCCGACGAAGTGCAGCTCCATCAAAAAGAGGCACCAGGACAACCGGAGCCGGTACGTACCGTTGATGCGCT
CGGTAATGTCCATTACGACGATAACCAGGTGATCCTCAAAGGGCCGAAAGGCTGGGCGAATCTGAACACCAAAGATACCA
ATGTCTGGGAAGGTGATTACCAGATGGTGGGTCGTCAGGGCCGCGGTAAAGCGGACCTGATGAAACAACGTGGCGAAAAC
CGCTATACCATTCTGGATAACGGTAGCTTTACCTCCTGTCTGCCGGGTTCTGACACCTGGAGCGTGGTAGGTAGCGAAAT
TATTCATGACCGCGAAGAACAAGTTGCGGAGATCTGGAACGCCCGCTTTAAGGTGGGTCCGGTACCGATCTTTTATAGCC
CCTATTTGCAGTTGCCGGTGGGTGACAAACGTCGCTCTGGTTTCTTGATCCCGAACGCTAAGTACACCACTACCAACTAC
TTTGAGTTCTACCTTCCATATTACTGGAACATCGCGCCAAATATGGATGCCACCATCACGCCGCATTATATGCATCGTCG
TGGCAATATCATGTGGGAGAACGAATTCCGCTACCTCTCCCAGGCGGGCGCTGGCTTGATGGAACTGGACTATCTGCCTT
CAGATAAAGTCTATGAAGATGAACACCCGAACGATGACAGTTCACGTCGTTGGTTATTCTACTGGAACCACTCCGGGGTC
ATGGATCAGGTGTGGCGTTTCAACGTCGACTACACCAAGGTCAGCGATCCTAGCTACTTCAATGATTTCGATAACAAGTA
CGGTTCCAGTACTGACGGCTACGCAACGCAAAAATTCAGCGTTGGCTATGCGGTGCAAAACTTCAATGCCACCGTTTCAA
CCAAGCAGTTCCAGGTCTTCAGCGAGCAGAACACCAGTAGCTACTCGGCAGAGCCGCAGTTAGACGTTAACTACTACCAG
AATGATGTTGGTCCGTTTGATACGCGTATTTACGGCCAGGCAGTGCACTTTGTTAACACCAGAGACGACATGCCTGAAGC
AACCCGTGTTCACCTGGAACCGACCATCAATTTGCCGCTCTCTAATAACTGGGGCAGCATCAATACCGAAGCGAAGTTGC
TGGCAACCCATTATCAGCAAACTAATCTTGACTGGTATAACTCCAGAAACACAACCAAGCTGGACGAATCCGTTAACCGC
GTAATGCCGCAATTTAAAGTTGACGGCAAAATGGTCTTCGAACGTGATATGGAAATGCTGGCTCCGGGTTATACCCAAAC
GCTGGAACCGCGCGCGCAGTATTTATACGTGCCGTATCGCGATCAGAGCGACATCTATAACTACGACTCGTCTCTGCTGC
AATCTGACTACTCTGGCCTGTTCCGGGACCGGACTTACGGCGGTCTTGACCGTATTGCCTCCGCTAACCAGGTGACGACC
GGTGTCACATCTCGCATATATGATGATGCTGCCGTTGAACGTTTTAATATTTCCGTTGGTCAAATCTACTATTTCACGGA
GTCTCGCACTGGCGATGACAACATAACATGGGAGAATGACGACAAAACGGGCTCACTGGTGTGGGCAGGCGATACTTACT
GGCGTATCTCCGAGCGTTGGGGATTGCGTGGCGGGATTCAGTACGATACACGTCTGGATAACGTAGCGACCAGTAACTCC
AGCATTGAATACCGTCGGGATGAAGACCGTCTGGTACAGCTGAACTACCGTTACGCCAGCCCGGAATATATTCAGGCTAC
GCTGCCTAAGTACTATTCCACGGCTGAGCAATATAAGAATGGTATTTCGCAGGTAGGTGCCGTCGCCAGCTGGCCAATTG
CCGATCGTTGGTCAATTGTTGGGGCCTACTACTACGACACCAATGCGAACAAGCAAGCCGACTCTATGTTAGGTGTGCAA
TACAGCTCCTGCTGCTATGCAATTCGCGTCGGTTACGAGCGGAAGCTGAACGGTTGGGATAACGATAAACAACATGCGGT
ATATGACAACGCAATCGGCTTTAACATCGAACTTCGCGGCCTGAGCTCCAACTACGGTCTGGGTACGCAAGAGATGCTGC
GTTCGAACATTCTGCCGTATCAAAACACTTTGTGA

Upstream 100 bases:

>100_bases
TTCGGACAGGATTAACACTAGCGTAGAGATGACGAGTACGTTAGTCTCTGACCGTTTGTCACGCGCAACGTTACCGATGA
TGGAACAATAAAATCAACGT

Downstream 100 bases:

>100_bases
TTTGTTGATTTACCACGTAATCCGCAGTGCGGTTAATTGAAATGGAAAAAGTATGAAGAACTGGAAAACGCTGCTTCTCG
GTATCGCCATGATCGCGAAT

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 784; Mature: 784

Protein sequence:

>784_residues
MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNS
RLQADEVQLHQKEAPGQPEPVRTVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNY
FEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGV
MDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQ
NDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTEAKLLATHYQQTNLDWYNSRNTTKLDESVNR
VMPQFKVDGKMVFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTT
GVTSRIYDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNS
SIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQ
YSSCCYAIRVGYERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL

Sequences:

>Translated_784_residues
MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNS
RLQADEVQLHQKEAPGQPEPVRTVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNY
FEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGV
MDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQ
NDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTEAKLLATHYQQTNLDWYNSRNTTKLDESVNR
VMPQFKVDGKMVFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTT
GVTSRIYDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNS
SIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQ
YSSCCYAIRVGYERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL
>Mature_784_residues
MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNS
RLQADEVQLHQKEAPGQPEPVRTVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNY
FEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGV
MDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQ
NDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTEAKLLATHYQQTNLDWYNSRNTTKLDESVNR
VMPQFKVDGKMVFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTT
GVTSRIYDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNS
SIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQ
YSSCCYAIRVGYERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL

Specific function: Together with lptE, is involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=784, Percent_Identity=100, Blast_Score=1629, Evalue=0.0,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 89672; Mature: 89672

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINADHAK
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCEECCCCCCCCEEEECCCCC
GDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVRTVDALGNVHYDDNQVILK
CCCCCCCEEECEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCEECCCEEEEE
GPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGENRYTILDNGSFTSCLPGSDTW
CCCCCCCCCCCCCEEECCHHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCCEECCCCCHH
SVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNY
HHHHHHHHHCHHHHHHHHHCCCEEECCEEEEECCEEECCCCCCCCCCEECCCCCEEECCE
FEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYED
EEEEEEEEEEECCCCCCEECHHHHHHCCCEEEEHHHHHHHHCCCCEEEEECCCCCCCCCC
EHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTDGYATQKFS
CCCCCCCCCEEEEEECCCCHHHHHHEECCCEEECCCCCHHCHHHHHCCCCCCCCEEEEEE
VGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQNDVGPFDTRIYGQAVHFVNT
EEEEEECCCCEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEEECC
RDDMPEATRVHLEPTINLPLSNNWGSINTEAKLLATHYQQTNLDWYNSRNTTKLDESVNR
CCCCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEEEHHHHCCCCEECCCCCCHHHHHHHH
VMPQFKVDGKMVFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGL
HCCCEECCCEEEEHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
FRDRTYGGLDRIASANQVTTGVTSRIYDDAAVERFNISVGQIYYFTESRTGDDNITWEND
HHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHEEEECEEEEEEEEECCCCCCCCCEECCC
DKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYAS
CCCCCEEECCCCEEEEECCCCCCCCCEECCCCCCEECCCCCEEEECCCCEEEEEEEEECC
PEYIQATLPKYYSTAEQYKNGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCEEEEEEEEECCCCCCHHHHHCCC
YSSCCYAIRVGYERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPY
HHCEEEEEEECCEEECCCCCCCCCCEEECCCEEEEEEEEECCCCCCCCHHHHHHHCCCCC
QNTL
CCCC
>Mature Secondary Structure
MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINADHAK
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCEECCCCCCCCEEEECCCCC
GDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVRTVDALGNVHYDDNQVILK
CCCCCCCEEECEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCEECCCEEEEE
GPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGENRYTILDNGSFTSCLPGSDTW
CCCCCCCCCCCCCEEECCHHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCCEECCCCCHH
SVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNY
HHHHHHHHHCHHHHHHHHHCCCEEECCEEEEECCEEECCCCCCCCCCEECCCCCEEECCE
FEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYED
EEEEEEEEEEECCCCCCEECHHHHHHCCCEEEEHHHHHHHHCCCCEEEEECCCCCCCCCC
EHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTDGYATQKFS
CCCCCCCCCEEEEEECCCCHHHHHHEECCCEEECCCCCHHCHHHHHCCCCCCCCEEEEEE
VGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQNDVGPFDTRIYGQAVHFVNT
EEEEEECCCCEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEEECC
RDDMPEATRVHLEPTINLPLSNNWGSINTEAKLLATHYQQTNLDWYNSRNTTKLDESVNR
CCCCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEEEHHHHCCCCEECCCCCCHHHHHHHH
VMPQFKVDGKMVFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGL
HCCCEECCCEEEEHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
FRDRTYGGLDRIASANQVTTGVTSRIYDDAAVERFNISVGQIYYFTESRTGDDNITWEND
HHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHEEEECEEEEEEEEECCCCCCCCCEECCC
DKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYAS
CCCCCEEECCCCEEEEECCCCCCCCCEECCCCCCEECCCCCEEEECCCCEEEEEEEEECC
PEYIQATLPKYYSTAEQYKNGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCEEEEEEEEECCCCCCHHHHHCCC
YSSCCYAIRVGYERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPY
HHCEEEEEEECCEEECCCCCCCCCCEEECCCEEEEEEEEECCCCCCCCHHHHHHHCCCCC
QNTL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA