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The map label for this gene is caiT [H]

Identifier: 218703301

GI number: 218703301

Start: 44853

End: 46367

Strand: Reverse

Name: caiT [H]

Synonym: ECUMN_0042

Alternate gene names: 218703301

Gene position: 46367-44853 (Counterclockwise)

Preceding gene: 218703310

Following gene: 218703300

Centisome position: 0.89

GC content: 52.08

Gene sequence:

>1515_bases
ATGAAGAATGAAAAGAGAAAAACGGGAATAGAACCGAAGGTTTTCTTTCCGCCGTTAATAATCGTCGGCATACTTTGTTG
GCTTACAGTCAGAGATCTGGATGCGGCGAATGTCGTTATTAATGCTGTATTCAGTTACGTCACCAATGTATGGGGATGGG
CATTTGAATGGTATATGGTGGTGATGCTTTTCGGTTGGTTCTGGCTGGTGTTTGGCCCGTATGCCAAAAAGCGTTTAGGT
AACGAACCACCAGAATTTAGTACCGCCAGTTGGATCTTTATGATGTTCGCCTCCTGTACGTCTGCTGCCGTACTGTTCTG
GGGATCGATTGAGATCTACTACTACATCTCCACCCCGCCGTTTGGCTTAGAACCGAACTCGACAGGGGCGAAAGAGTTGG
GGCTGGCTTACAGCTTGTTCCACTGGGGACCTCTGCCGTGGGCCACTTATAGCTTCCTTTCTGTCGCCTTCGCTTACTTC
TTCTTTGTCCGCAAAATGGAAGTGATTCGCCCCAGCTCCACCCTGGTGCCGCTGGTGGGTGAAAAACACGCCAAAGGGTT
GTTCGGTACCATCGTCGACAACTTCTATCTCGTCGCCTTGATTTTCGCGATGGGTACCAGTCTGGGCCTTGCCACGCCGC
TGGTGACCGAGTGTATGCAATGGTTGTTTGGCATTCCGCATACCCTCCAACTGGACGCTATCATCATTACCTGCTGGATT
ATCCTCAACGCCATTTGCGTCGCCTGCGGTCTGCAAAAAGGGGTACGTATCGCCAGTGACGTGCGTAGTTACCTGAGCTT
CCTGATGCTGGGTTGGGTGTTCATTGTCAGCGGTGCCAGCTTCATCATGAACTACTTCACCGATTCGGTGGGGATGTTGC
TGATGTATCTGCCGCGCATGTTGTTCTATACCGATCCCATCGCTAAAGGCGGCTTCCCGCAGGGCTGGACCGTGTTCTAC
TGGGCATGGTGGGTGATTTACGCTATCCAGATGAGTATCTTCCTCGCCCGCATCTCCCGTGGTCGTACCGTGCGTGAACT
GTGCTTCGGCATGGTGCTGGGGCTGACAGCGTCAACCTGGATCCTGTGGACTGTACTCGGTAGTAACACTCTGCTGTTGA
TAGATAAAAACATCATCAACATTCCAAATCTGATCGAACAGTACGGTGTGGCGCGCGCCATCATTGAAACCTGGGCCGCT
CTGCCACTCAGCACCGCCACCATGTGGGGCTTCTTCATCCTCTGCTTTATTGCCACCGTTACGCTGGTTAACGCCTGCTC
TTATACCCTGGCGATGTCCACTTGCCGCGAAGTACGCGATGGTGAAGAACCACCGCTGCTGGTGCGTATCGGCTGGTCAA
TTCTGGTTGGCATTATCGGTATTGTTCTGCTGGCGCTCGGCGGCCTGAAACCGATTCAAACCGCCATTATCGCCGGAGGA
TGCCCGCTGTTCTTCGTCAACATTATGGTGACGCTCTCCTTTATTAAAGACGCGAAACAGAACTGGAAAGATTAA

Upstream 100 bases:

>100_bases
AACGCGTCCACGAGGTTAATAATAATTATATTAAATGTTAACAAAAATAAAACAAACGGGAACGCAAAATAAATATTCGT
TTTCACAGTGGAATTAACTC

Downstream 100 bases:

>100_bases
TTAACCCCCAAAATATCAAGAGGTTGAAAGATGGATTTTAATTTAAATGATGAGCAGGAACTGTTTGTCGCCGGTATCCG
CGAACTTATGGCCAGCGAAA

Product: L-carnitine/gamma-butyrobetaine antiporter

Products: Proton [Cytoplasm]; L-carnitine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVFSYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLG
NEPPEFSTASWIFMMFASCTSAAVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTECMQWLFGIPHTLQLDAIIITCWI
ILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFY
WAWWVIYAIQMSIFLARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVARAIIETWAA
LPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRIGWSILVGIIGIVLLALGGLKPIQTAIIAGG
CPLFFVNIMVTLSFIKDAKQNWKD

Sequences:

>Translated_504_residues
MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVFSYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLG
NEPPEFSTASWIFMMFASCTSAAVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTECMQWLFGIPHTLQLDAIIITCWI
ILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFY
WAWWVIYAIQMSIFLARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVARAIIETWAA
LPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRIGWSILVGIIGIVLLALGGLKPIQTAIIAGG
CPLFFVNIMVTLSFIKDAKQNWKD
>Mature_504_residues
MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVFSYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLG
NEPPEFSTASWIFMMFASCTSAAVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTECMQWLFGIPHTLQLDAIIITCWI
ILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFY
WAWWVIYAIQMSIFLARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVARAIIETWAA
LPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRIGWSILVGIIGIVLLALGGLKPIQTAIIAGG
CPLFFVNIMVTLSFIKDAKQNWKD

Specific function: Catalyzes the exchange of L-carnitine for gamma- butyrobetaine and related betaines [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family. CaiT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786224, Length=504, Percent_Identity=100, Blast_Score=1016, Evalue=0.0,
Organism=Escherichia coli, GI1788102, Length=438, Percent_Identity=29.4520547945205, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1786506, Length=514, Percent_Identity=26.0700389105058, Blast_Score=151, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56588; Mature: 56588

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVFSYVTNVWGWAFEWYMV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSAAVLFWGSIEIYYYISTPP
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCC
FGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYFFFVRKMEVIRPSSTLVPLVG
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
EKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTECMQWLFGIPHTLQLDAIIITCWI
CHHHCHHHHHHHHHHHHHHHHHHHHCHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGASFIMNYFTDSVGMLLMYLPRM
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIFLARISRGRTVRELCFGMVLGLTASTW
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ILWTVLGSNTLLLIDKNIINIPNLIEQYGVARAIIETWAALPLSTATMWGFFILCFIATV
HHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TLVNACSYTLAMSTCREVRDGEEPPLLVRIGWSILVGIIGIVLLALGGLKPIQTAIIAGG
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
CPLFFVNIMVTLSFIKDAKQNWKD
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVFSYVTNVWGWAFEWYMV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSAAVLFWGSIEIYYYISTPP
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCC
FGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYFFFVRKMEVIRPSSTLVPLVG
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
EKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTECMQWLFGIPHTLQLDAIIITCWI
CHHHCHHHHHHHHHHHHHHHHHHHHCHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGASFIMNYFTDSVGMLLMYLPRM
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIFLARISRGRTVRELCFGMVLGLTASTW
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ILWTVLGSNTLLLIDKNIINIPNLIEQYGVARAIIETWAALPLSTATMWGFFILCFIATV
HHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TLVNACSYTLAMSTCREVRDGEEPPLLVRIGWSILVGIIGIVLLALGGLKPIQTAIIAGG
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
CPLFFVNIMVTLSFIKDAKQNWKD
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-carnitine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-carnitine [Periplasm] = Proton [Cytoplasm] + L-carnitine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA