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The map label for this gene is Rz

Identifier: 218703094

GI number: 218703094

Start: 5073270

End: 5073749

Strand: Direct

Name: Rz

Synonym: ECIAI39_4876

Alternate gene names: 218703094

Gene position: 5073270-5073749 (Clockwise)

Preceding gene: 218703093

Following gene: 218703095

Centisome position: 98.85

GC content: 52.92

Gene sequence:

>480_bases
GTGGGGGCAACAATGAGCAGAGTAACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCGTGGGCGGT
CAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGAGATAAAAAAGTCAGTGAGCTGAAGCAGGCGACCGCCA
CCATTACTGACATGCAGCAGCGCCAGCGTTCTGCTGATGCACTCGATGCTAAATACACGAAGGAGTTAGCTGATGCGAAA
GCTGAAAATGATGCTCTTCGGCGCAAGCTTGATAATGGTGGTCGGGTGTTCGTCAAAGGAAAATGCCCTGTGCCATCCTC
AGCCGAAACCTCCAGCGCCTCCGGCATGGGCAATGATGCCACCGTCGAACTCTCTCCAGTTGCTGGACGAAACGTTCTCG
GTATCCGGGACGGAATTATCCGCGACCAAACAGCACTGAGAACGCTTCAGGAATACATCAGGACGCAATGCCTTCGATGA

Upstream 100 bases:

>100_bases
TCAAAGGCGCATGTGACCAGCTACGTCGCTGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAG
ATTGAGCGTGAAGTCTGTTT

Downstream 100 bases:

>100_bases
TAGCGATAATTTTACTCATCATCCTTCACATCTGGCTCTGTAGACAGGATGGTGATCACTTCTGGAGTGAATCCAGATTA
AACATCTCATTGCTGATGCT

Product: Endopeptidase from phage origin (Lysis protein Rz)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MGATMSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKKVSELKQATATITDMQQRQRSADALDAKYTKELADAK
AENDALRRKLDNGGRVFVKGKCPVPSSAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR

Sequences:

>Translated_159_residues
MGATMSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKKVSELKQATATITDMQQRQRSADALDAKYTKELADAK
AENDALRRKLDNGGRVFVKGKCPVPSSAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR
>Mature_158_residues
GATMSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKKVSELKQATATITDMQQRQRSADALDAKYTKELADAKA
ENDALRRKLDNGGRVFVKGKCPVPSSAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR

Specific function: Necessary for host cell lysis. It is believed to code for an endopeptidase that cleaves the amino-carboxyl cross-link between the diaminopimelic acid and D-alanine residues in the murein component of the bacterial cell wall [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786769, Length=154, Percent_Identity=60.3896103896104, Blast_Score=145, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004929 [H]

Pfam domain/function: PF03245 Phage_lysis [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 17416; Mature: 17285

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGATMSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKKVSELKQATATITDMQQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQRSADALDAKYTKELADAKAENDALRRKLDNGGRVFVKGKCPVPSSAETSSASGMGNDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCC
TVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR
EEEECCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GATMSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKKVSELKQATATITDMQQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQRSADALDAKYTKELADAKAENDALRRKLDNGGRVFVKGKCPVPSSAETSSASGMGNDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCC
TVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR
EEEECCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]