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The map label for this gene is intB

Identifier: 218702973

GI number: 218702973

Start: 4950895

End: 4952085

Strand: Direct

Name: intB

Synonym: ECIAI39_4743

Alternate gene names: 218702973

Gene position: 4950895-4952085 (Clockwise)

Preceding gene: 218702971

Following gene: 218702975

Centisome position: 96.47

GC content: 45.51

Gene sequence:

>1191_bases
ATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGC
ACTTGGGGTTTATCCTGAAATCACACTAGCGGATGCCAGAGTACGTCGTGACGAGGCGCGTAACCTGCTTGCGAATGGCG
TCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGTGG
CATGGCACCAATAAAAAGTGGTCTGAAGATCACGCCCATCGTGTGCTAAAAAGTCTTGAAGATAATCTTTTTGCAGCGCT
TGGTGAACGTAATATCGCTGAGTTAAAAACTCGAGATTTATTAGCACCTATTAAGGCCGTAGAAATGTCTGGACGTCTTG
AAGTGGCCGCTCGTCTTCAGCAGCGCACTACAGCCATCATGCGCTATGCAGTGCAAAGTGGGTTAATTGATTATAACCCG
GCACAAGAGATGGCTGGGGCGGTTGCTTCCTGTAATCGACAACATCGTCCCGCGCTTGAATTAAAGCGCATCCCTGAGTT
GCTTACAAAAATAGATAGCTATACTGGTAGGCCGCTAACCCGATGGGCGATAGAACTCACTTTGCTGATCTTTATTCGGT
CCAGTGAGCTGCGTTTTGCTCGTTGGTCAGAGATCGATTTCGAAGCGTCTATATGGACTATCCCACCGGAGCGGGAGCCT
ATTCCTGGAGTGAAACATTCCCATAGAGGCTCAAAAATGCGTACAACGCATCTAGTGCCTCTTTCAACGCAAGCTCTTGC
AATTTTAAAGCAGATAAAACAGTTTTATGGGGCCCATGACTTGATATTTATTGGTGATCACGATTCGCACAAACCCATGA
GTGAGAATACGGTAAATAGTGCGTTACGGGTCATGGGGTATGATACAAAAGTAGAGGTTTGTGGTCATGGCTTTCGAACA
ATGGCCTGTAGTTCATTGGTCGAATCAGGTTTGTGGTCTCGTGATGCTGTTGAACGTCAGATGAGCCACATGGAGCGAAA
TTCAGTGAGGGCCGCGTATATCCATAAAGCAGAGCATCTGGAAGAACGGCGATTGATGCTACAGTGGTGGGCCGATTTTC
TGGATGTAAACAGAGAAAGATTTATCAGTCCATTTGAATATGCAAAGATTAATAATCCATTAAAACAGTAA

Upstream 100 bases:

>100_bases
TTGTACCAACAGGGAGGGAATACGCATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATA
AGCTGACTGATGGTGGCGGT

Downstream 100 bases:

>100_bases
TCATCCCGGGCAAATGCCCGGGAATTATTCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATT
TACTATTTACCGCTCCCTGC

Product: putative integrase; KpLE2 phage-like element

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MHLLVHPNGSKYWRLQYRYEGKQKMLALGVYPEITLADARVRRDEARNLLANGVDPGDKKKNDKVEQSKARTFKEVAIEW
HGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEVAARLQQRTTAIMRYAVQSGLIDYNP
AQEMAGAVASCNRQHRPALELKRIPELLTKIDSYTGRPLTRWAIELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREP
IPGVKHSHRGSKMRTTHLVPLSTQALAILKQIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDTKVEVCGHGFRT
MACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLMLQWWADFLDVNRERFISPFEYAKINNPLKQ

Sequences:

>Translated_396_residues
MHLLVHPNGSKYWRLQYRYEGKQKMLALGVYPEITLADARVRRDEARNLLANGVDPGDKKKNDKVEQSKARTFKEVAIEW
HGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEVAARLQQRTTAIMRYAVQSGLIDYNP
AQEMAGAVASCNRQHRPALELKRIPELLTKIDSYTGRPLTRWAIELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREP
IPGVKHSHRGSKMRTTHLVPLSTQALAILKQIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDTKVEVCGHGFRT
MACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLMLQWWADFLDVNRERFISPFEYAKINNPLKQ
>Mature_396_residues
MHLLVHPNGSKYWRLQYRYEGKQKMLALGVYPEITLADARVRRDEARNLLANGVDPGDKKKNDKVEQSKARTFKEVAIEW
HGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEVAARLQQRTTAIMRYAVQSGLIDYNP
AQEMAGAVASCNRQHRPALELKRIPELLTKIDSYTGRPLTRWAIELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREP
IPGVKHSHRGSKMRTTHLVPLSTQALAILKQIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDTKVEVCGHGFRT
MACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLMLQWWADFLDVNRERFISPFEYAKINNPLKQ

Specific function: Unknown

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=379, Percent_Identity=35.0923482849604, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI145693166, Length=378, Percent_Identity=34.6560846560847, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788690, Length=377, Percent_Identity=31.8302387267904, Blast_Score=196, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45627; Mature: 45627

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLLVHPNGSKYWRLQYRYEGKQKMLALGVYPEITLADARVRRDEARNLLANGVDPGDKK
CEEEEECCCCCEEEEEEEECCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCC
KNDKVEQSKARTFKEVAIEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDL
CCCHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LAPIKAVEMSGRLEVAARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHH
LKRIPELLTKIDSYTGRPLTRWAIELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREP
HHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCCEEECCCCCCCEEEEECCCCCCC
IPGVKHSHRGSKMRTTHLVPLSTQALAILKQIKQFYGAHDLIFIGDHDSHKPMSENTVNS
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHH
ALRVMGYDTKVEVCGHGFRTMACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHL
HHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
EERRLMLQWWADFLDVNRERFISPFEYAKINNPLKQ
HHHHHHHHHHHHHHHCCHHHCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MHLLVHPNGSKYWRLQYRYEGKQKMLALGVYPEITLADARVRRDEARNLLANGVDPGDKK
CEEEEECCCCCEEEEEEEECCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCC
KNDKVEQSKARTFKEVAIEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDL
CCCHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LAPIKAVEMSGRLEVAARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHH
LKRIPELLTKIDSYTGRPLTRWAIELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREP
HHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCCEEECCCCCCCEEEEECCCCCCC
IPGVKHSHRGSKMRTTHLVPLSTQALAILKQIKQFYGAHDLIFIGDHDSHKPMSENTVNS
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHH
ALRVMGYDTKVEVCGHGFRTMACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHL
HHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
EERRLMLQWWADFLDVNRERFISPFEYAKINNPLKQ
HHHHHHHHHHHHHHHCCHHHCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]