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The map label for this gene is sgaE

Identifier: 218702895

GI number: 218702895

Start: 4863166

End: 4863852

Strand: Direct

Name: sgaE

Synonym: ECIAI39_4663

Alternate gene names: 218702895

Gene position: 4863166-4863852 (Clockwise)

Preceding gene: 218702894

Following gene: 218702897

Centisome position: 94.76

GC content: 55.46

Gene sequence:

>687_bases
ATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGATCTGCCGCGTTACGGGCTGGTGACCTTTACCTGGGGCAA
CGTCAGCGCCATTGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCATTGCCTACGAAAGCATGAAAGCGGCCG
ATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCGTCTTCCGACACCGCGACGCATCTCGAA
CTCTACCGTCGTTATCCGTCGCTTGGCGGTATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCT
GGCGATCCCGGCATTAGGCACCACGCACGCTGACTACTTCTTTGGTGATATCCCGTGTACGCGTGGATTAAGCGAAGAAG
AGGTGCAGGGTGAGTATGAACTGAACACTGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCG
GGAATTGTGGTGTATCAGCACGGGCCGTTTGCCTGGGGGAAAGATGCCCACGATGCGGTGCATAACGCGGTGGTGATGGA
AGAAGTGGCGAAAATGGCGTGGATTGCGCGCAGTATAAACCCGCAACTGAATCACATCGACAGCTATCTTATGAATAAAC
ACTTCATGCGTAAGCATGGTCCCAACGCTTATTACGGGCAGAAGTAA

Upstream 100 bases:

>100_bases
TGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCA
GGCATGGTGGAGGCGGCATA

Downstream 100 bases:

>100_bases
AACACGCGCTGCGGAAATTTCCTTCTTCGGGAGATAACTGGTCTGATTCCGCAGCCGTTTTTCAAAAAAAGCCCCCTGCG
AAGGGGGCAAAGCAAACTAT

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: L-ascorbate utilization protein F; Phosphoribulose isomerase [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGIAYESMKAADMVVVDMSGKVVEGEYRPSSDTATHLE
LYRRYPSLGGIVHTHSTHATAWAQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRKHGPNAYYGQK

Sequences:

>Translated_228_residues
MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGIAYESMKAADMVVVDMSGKVVEGEYRPSSDTATHLE
LYRRYPSLGGIVHTHSTHATAWAQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRKHGPNAYYGQK
>Mature_228_residues
MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGIAYESMKAADMVVVDMSGKVVEGEYRPSSDTATHLE
LYRRYPSLGGIVHTHSTHATAWAQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRKHGPNAYYGQK

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790642, Length=228, Percent_Identity=97.8070175438597, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1786247, Length=230, Percent_Identity=60, Blast_Score=294, Evalue=4e-81,
Organism=Escherichia coli, GI1790008, Length=230, Percent_Identity=60.8695652173913, Blast_Score=292, Evalue=1e-80,
Organism=Escherichia coli, GI1789164, Length=138, Percent_Identity=34.7826086956522, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: =5.1.3.4 [H]

Molecular weight: Translated: 25310; Mature: 25310

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGIAYESMKAADMVVVDM
CHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCCEEEECCCCCCHHCCCCCEEEEEEC
SGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAWAQAGLAIPALGTTHADYF
CCEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCEECCCCCCCCCCEE
FGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTPGIVVYQHGPFAWGKDAHDAV
ECCCCCCCCCCHHHCCCEEEECCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCHHHHH
HNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRKHGPNAYYGQK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGIAYESMKAADMVVVDM
CHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCCEEEECCCCCCHHCCCCCEEEEEEC
SGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAWAQAGLAIPALGTTHADYF
CCEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCEECCCCCCCCCCEE
FGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTPGIVVYQHGPFAWGKDAHDAV
ECCCCCCCCCCHHHCCCEEEECCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCHHHHH
HNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRKHGPNAYYGQK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA