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The map label for this gene is insK [C]

Identifier: 218702801

GI number: 218702801

Start: 4771720

End: 4772607

Strand: Direct

Name: insK [C]

Synonym: ECIAI39_4565

Alternate gene names: 218702801

Gene position: 4771720-4772607 (Clockwise)

Preceding gene: 218702800

Following gene: 218702815

Centisome position: 92.98

GC content: 53.49

Gene sequence:

>888_bases
ATGCCACTGCTGGATAAGCTGCGTGAGCAGTACGGGGTCGGACCTGTATGCAGTGAACTGCATATTGCCCCGTCAACGTA
TTACCACTGTCAGCAGCAGCGGCATCATCCTGATAAACGCTGTACCCGTGCGCAGCGCGATGACTGGCTGAAGAGAGAGA
TACAGCGCGTATACGATGAAAATCATCAGGTGTACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGAATCAGG
GTGGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGCACTAC
CGTCAGCCGGAAAACCGTTGCCACAGGTGACCGCGTAAACCGTCAGTTCGTGGCAGAACGTCCTGACCAGCTGTGGGTGG
CTGATTTTACTTACGTCAGCACATGGCAGGGCTTCGTCTATGTGGCGTTTATCATTGATGTGTTTGCTGGATACATCGTG
GGGTGGCGGGTCTCATCGTCTATGGAAACGACATTCGTGCTGGATGCGCTGGAGCAGGCGTTGTGGGCCCGTCGTCCGTC
TGGCACCATCCATCACAGCGATAAAGGCTCTCAGTATGTGTCACTGGCCTATACGGAGCGACTAAAAGAAGCCGGATTAC
TGGCATCAACAGGAAGTACAGGCGACTCGTATGACAACGCGATGGCTGAGAGCATCAATGGTCTTTACAAAGCGGAGGTA
ATACACCGTAAGAGCTGGAAAAACCGTGCAGAAGTGGAACTGGCCACACTAACGTGGGTGGACTGGTATAACAATCGACG
ATTACTGGGAAGGCTGGGCCATACTCCTCCGGCAGAAGCAGAAAAAGCTTATTATGCTTCCATCGGAAACAATGATCTGG
CAGCCTGA

Upstream 100 bases:

>100_bases
TGGAACGTGAAAATCGTGAACTGCGCCGCAGTAACGATATCCTTCGCCAGGCTTCCGCTTATTTTGCGAAGGCGGAGTTC
GACCGCCTCTGGAAAAAATA

Downstream 100 bases:

>100_bases
GTTCACAGATAAAACACTCTCCAGGAAACCCGGGGCGGTTCAGCGTTACAGTTATTCAGAAGCAAATGTTCTGCTTCCTG
GCTGATGCTGTTGTTCTGAT

Product: transposase ORF B, IS629

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRCTRAQRDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIR
VARCTVARLMAVMGLAGVLRGKKVRTTVSRKTVATGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIV
GWRVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAMAESINGLYKAEV
IHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPAEAEKAYYASIGNNDLAA

Sequences:

>Translated_295_residues
MPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRCTRAQRDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIR
VARCTVARLMAVMGLAGVLRGKKVRTTVSRKTVATGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIV
GWRVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAMAESINGLYKAEV
IHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPAEAEKAYYASIGNNDLAA
>Mature_294_residues
PLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRCTRAQRDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRV
ARCTVARLMAVMGLAGVLRGKKVRTTVSRKTVATGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVG
WRVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVI
HRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPAEAEKAYYASIGNNDLAA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=252, Percent_Identity=51.5873015873016, Blast_Score=249, Evalue=2e-66,
Organism=Escherichia coli, GI1789981, Length=286, Percent_Identity=28.6713286713287, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788405, Length=291, Percent_Identity=28.1786941580756, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787263, Length=291, Percent_Identity=28.1786941580756, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1786753, Length=291, Percent_Identity=28.1786941580756, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1786570, Length=291, Percent_Identity=28.1786941580756, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1786490, Length=291, Percent_Identity=28.1786941580756, Blast_Score=114, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33698; Mature: 33566

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRCTRAQRDDWLKREIQRVYDE
CCHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
NHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVSRKTVATGDRVN
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHH
RQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQA
HHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHH
LWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAMAESINGLYKAEV
HHHCCCCCCEEECCCCCCEEHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHH
IHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPAEAEKAYYASIGNNDLAA
HHHHHCCCCCCEEEEEEEEEHHCCCCHHHHHCCCCCCCCHHHHHEEECCCCCCCC
>Mature Secondary Structure 
PLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRCTRAQRDDWLKREIQRVYDE
CHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
NHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVSRKTVATGDRVN
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHH
RQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQA
HHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHH
LWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAMAESINGLYKAEV
HHHCCCCCCEEECCCCCCEEHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHH
IHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPAEAEKAYYASIGNNDLAA
HHHHHCCCCCCEEEEEEEEEHHCCCCHHHHHCCCCCCCCHHHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2159625; 2824781 [H]