Click here to switch to the map view.

The map label for this gene is aceK

Identifier: 218702649

GI number: 218702649

Start: 4604786

End: 4606510

Strand: Direct

Name: aceK

Synonym: ECIAI39_4404

Alternate gene names: 218702649

Gene position: 4604786-4606510 (Clockwise)

Preceding gene: 218702648

Following gene: 218702651

Centisome position: 89.73

GC content: 53.68

Gene sequence:

>1725_bases
ATGCCGCGTGGCCTGGAATTACTGATTGCACAAACCATTCTGCAAGGCTTCGACGCTCAGTATGGTCGATTCCTCGAAGT
GACTTCCGGGGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCTATCTTT
ACGATCATCACGTGGGTCTGGTGGTGGAGCAACTGCGCTGCATTACCAACGGCCAAAGCACGGACGCGGCATTTTTACTG
CGCGTCAAAGAGCATTACACGCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTA
CTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCCCAGCCAGAGCGCCGTTTTCGTA
CCATTCCGCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTGCTGATGCGTGTTATCAGCGACCTG
CCGTTGCGTCTGCGCTGGCAGAATAAAAGCCGTGACATTCATTACATCGTTCGCCATCTGACGGAAACGCTGGGGGCAGA
CAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTGGGCAAACTGA
TCACGCCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACTTGCCTG
ACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCACCGCTGCCCGCAGCGCT
GGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCTA
AAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGC
ATGGTGATGCTGGTGTTTACGTTGCCGGGTTTTGATCGGGTCTTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGA
GATGTCTGCCGCTCACGTTCGTGCCTGCTACCAACTGGTGAAAGAGCACGATCGTGTGGGCCGAATGGCGGACACCCAGG
AGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTCCAGGAAGCAGCGGAAAAA
ATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATCGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGA
ACAAGTGGAAGGTCAGCAGTTGCGCGATGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTT
TCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTGTTTTATGATTACGACGAAATTTGC
TACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTACCCGGAGGACGAACTTGCCAGCGAACCGTGGTACAG
CGTCTCGCCGGGCGATGTTTTCCCGGAAGAGTTTCGCCATTGGCTATGCGCCGACTCGCGTATTGGGCCACTGTTTGAAG
AGATGCACGCCGACCTGTTCCGCGCCGACTACTGGCGCGCGCTACAAAACCGCATTCGTGAAGGACATGTGGAAGATGTT
TATGCGTATCGGCGCAGGCAGAGGTTTAGCGTTCGATTCGTATAA

Upstream 100 bases:

>100_bases
GCTTGCGCGTCTTATCATGCCTACAACCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCT
GCTCCTGATGAGGGCGCTAA

Downstream 100 bases:

>100_bases
ATCAGAGGCGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAAC
TCGGTCACGCGGTCATCGGT

Product: bifunctional isocitrate dehydrogenase kinase/phosphatase protein

Products: NA

Alternate protein names: IDH kinase/phosphatase; IDHK/P [H]

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKNRIYLYDHHVGLVVEQLRCITNGQSTDAAFLL
RVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
PLRLRWQNKSRDIHYIVRHLTETLGADNLAESHLQVANELFYRNKAAWLVGKLITPSGTLPFLLPIHQTDDGELFIDTCL
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRG
MVMLVFTLPGFDRVFKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEK
ITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADSRIGPLFEEMHADLFRADYWRALQNRIREGHVEDV
YAYRRRQRFSVRFV

Sequences:

>Translated_574_residues
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKNRIYLYDHHVGLVVEQLRCITNGQSTDAAFLL
RVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
PLRLRWQNKSRDIHYIVRHLTETLGADNLAESHLQVANELFYRNKAAWLVGKLITPSGTLPFLLPIHQTDDGELFIDTCL
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRG
MVMLVFTLPGFDRVFKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEK
ITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADSRIGPLFEEMHADLFRADYWRALQNRIREGHVEDV
YAYRRRQRFSVRFV
>Mature_573_residues
PRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKNRIYLYDHHVGLVVEQLRCITNGQSTDAAFLLR
VKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDLP
LRLRWQNKSRDIHYIVRHLTETLGADNLAESHLQVANELFYRNKAAWLVGKLITPSGTLPFLLPIHQTDDGELFIDTCLT
TTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGM
VMLVFTLPGFDRVFKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEKI
TDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEICY
MTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADSRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVY
AYRRRQRFSVRFV

Specific function: Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source

COG id: COG4579

COG function: function code T; Isocitrate dehydrogenase kinase/phosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AceK family [H]

Homologues:

Organism=Escherichia coli, GI1790446, Length=573, Percent_Identity=99.1273996509599, Blast_Score=1181, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010452
- ProDom:   PD043552 [H]

Pfam domain/function: PF06315 AceK [H]

EC number: =2.7.11.5 [H]

Molecular weight: Translated: 67177; Mature: 67046

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKNRIYLYDHHVGL
CCCCHHHHHHHHHHHHCCHHCCCEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEECHHHH
VVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPER
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDLPLRLRWQNKSRDIHYIVRHL
EEEECCCCHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
TETLGADNLAESHLQVANELFYRNKAAWLVGKLITPSGTLPFLLPIHQTDDGELFIDTCL
HHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHEEECCCCCCCEEEEEEECCCCCEEEHHHH
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYRE
HHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCHHHHHH
YLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVIKDRFAPQKEMSAAHVRACYQLV
HHHHHHCCCHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEKITDLGEQIVIRHLYIERRMV
HHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCEE
YMTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADSRIGPLFEEMHADLF
EEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
RADYWRALQNRIREGHVEDVYAYRRRQRFSVRFV
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKNRIYLYDHHVGL
CCCHHHHHHHHHHHHCCHHCCCEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEECHHHH
VVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPER
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDLPLRLRWQNKSRDIHYIVRHL
EEEECCCCHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
TETLGADNLAESHLQVANELFYRNKAAWLVGKLITPSGTLPFLLPIHQTDDGELFIDTCL
HHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHEEECCCCCCCEEEEEEECCCCCEEEHHHH
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYRE
HHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCHHHHHH
YLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVIKDRFAPQKEMSAAHVRACYQLV
HHHHHHCCCHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEKITDLGEQIVIRHLYIERRMV
HHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCEE
YMTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADSRIGPLFEEMHADLF
EEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
RADYWRALQNRIREGHVEDVYAYRRRQRFSVRFV
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]