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The map label for this gene is aceB

Identifier: 218702645

GI number: 218702645

Start: 4600231

End: 4601832

Strand: Direct

Name: aceB

Synonym: ECIAI39_4400

Alternate gene names: 218702645

Gene position: 4600231-4601832 (Clockwise)

Preceding gene: 218702644

Following gene: 218702646

Centisome position: 89.64

GC content: 51.69

Gene sequence:

>1602_bases
ATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCATAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGC
CGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGC
AGCAGCAGGATATCGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTAGTGGTGCTGACTGGAAAATT
CGTGGTATTCCCGCGGACTTACAAGATCGTCGAGTCGAGATAACTGGCCCGGTTGAGCGCAAGATGGTGATCAACGCGCT
CAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCCCCGGACTGGAACAAAGTGATCGACGGGCAAA
TTAACCTGCGCGATGCGGTTAACGGCACCATCAGCTATACCAATGAAGCAGGCAAAATTTATCAGCTCAAGCCCAATCCA
GCGGTGTTGATTTGTCGGGTTCGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGCGGCGAAGCAATTCCTGGCAG
CCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTACCAGGCTCTGTTAGCAAAAGGCAGCGGTCCCTATTTCTATCTGC
CGAAAACCCAGTCCTGGCAGGAAGCGGCCTGGTGGAGCGAAGTCTTCAGCTATGCAGAAGATCGCTTTAATCTGCCGCGC
GGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGACGAAATCCTTCACGCGCTGCGTGA
CCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGTTATATCAAAACGTTGAAAAACTATCCCGATCGCG
TCCTGCCAGATAGACAGGCAGTGACGATGGATAAACCCTTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCAT
AAACGCGGTGCTTTTGCGATGGGCGGTATGGCGGCATTTATTCCGAGCAAAGATGAAGAGCGCAATAACCAGGTGCTCAA
CAAAGTAAAAGCGGATAAAGCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGATA
CGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATT
ACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCA
GTACATCGAAGCATGGATCTCCGGCAACGGCTGCGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTT
CCCGTACCTCAATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGACCAAAGCCTTGTTCCGC
CAGATGCTGGGCGAAGAGATGAAAGTCATTGCCAGCGAACTGGGCGAAGAACGTTTCTCCCAGGGGCGTTTTGACGATGC
CGCACGCTTGATGGAACAGATCACCACTTCCGATGAGTTAATTGATTTCCTGACCCTGCCAGGCTACCGCCTGTTAGCGT
AA

Upstream 100 bases:

>100_bases
CATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGCGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGAT
CCATGACGAGGAGCTGCACG

Downstream 100 bases:

>100_bases
ACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAA
CCGCGTTGGGAAGGCATTAC

Product: malate synthase

Products: NA

Alternate protein names: MSA [H]

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQRNKLLAARIQQQQDIDNGTLPDFISETASISGADWKI
RGIPADLQDRRVEITGPVERKMVINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQSWQEAAWWSEVFSYAEDRFNLPR
GTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGRWDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCH
KRGAFAMGGMAAFIPSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEVMREQDAPI
TADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIHHQKTLSNGKPVTKALFR
QMLGEEMKVIASELGEERFSQGRFDDAARLMEQITTSDELIDFLTLPGYRLLA

Sequences:

>Translated_533_residues
MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQRNKLLAARIQQQQDIDNGTLPDFISETASISGADWKI
RGIPADLQDRRVEITGPVERKMVINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQSWQEAAWWSEVFSYAEDRFNLPR
GTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGRWDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCH
KRGAFAMGGMAAFIPSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEVMREQDAPI
TADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIHHQKTLSNGKPVTKALFR
QMLGEEMKVIASELGEERFSQGRFDDAARLMEQITTSDELIDFLTLPGYRLLA
>Mature_532_residues
TEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQRNKLLAARIQQQQDIDNGTLPDFISETASISGADWKIR
GIPADLQDRRVEITGPVERKMVINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNPA
VLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQSWQEAAWWSEVFSYAEDRFNLPRG
TIKATLLIETLPAVFQMDEILHALRDHIVGLNCGRWDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHK
RGAFAMGGMAAFIPSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEVMREQDAPIT
ADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIHHQKTLSNGKPVTKALFRQ
MLGEEMKVIASELGEERFSQGRFDDAARLMEQITTSDELIDFLTLPGYRLLA

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=533, Percent_Identity=98.874296435272, Blast_Score=1100, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17561814, Length=516, Percent_Identity=51.1627906976744, Blast_Score=506, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71982926, Length=410, Percent_Identity=53.9024390243902, Blast_Score=419, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6324212, Length=528, Percent_Identity=45.4545454545455, Blast_Score=438, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6322222, Length=513, Percent_Identity=46.7836257309941, Blast_Score=437, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 60161; Mature: 60030

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQRNKLLAARIQQQQDIDN
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
GTLPDFISETASISGADWKIRGIPADLQDRRVEITGPVERKMVINALNANVKVFMADFED
CCCHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEECCHHHHHHHHHHCCCEEEEEECCCC
SLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNPAVLICRVRGLHLPEKHVTWR
CCCCCHHHHCCCCEEEHHHCCCEEEEECCCCEEEEECCCCEEEEEEECCCCCCCHHCCCC
GEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQSWQEAAWWSEVFSYAEDRFNLPR
CCCCCCHHHHHHHHHHHCCHHEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
GTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGRWDYIFSYIKTLKNYPDRVLPDRQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCE
VTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFIPSKDEERNNQVLNKVKADKALEANNG
EECCCHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
HDGTWIAHPGLADTAMAVFNDILGSRKNQLEVMREQDAPITADQLLAPCDGERTEEGMRA
CCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCC
NIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIHHQKTLSNGKPVTKALFR
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QMLGEEMKVIASELGEERFSQGRFDDAARLMEQITTSDELIDFLTLPGYRLLA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHCC
>Mature Secondary Structure 
TEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQRNKLLAARIQQQQDIDN
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
GTLPDFISETASISGADWKIRGIPADLQDRRVEITGPVERKMVINALNANVKVFMADFED
CCCHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEECCHHHHHHHHHHCCCEEEEEECCCC
SLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNPAVLICRVRGLHLPEKHVTWR
CCCCCHHHHCCCCEEEHHHCCCEEEEECCCCEEEEECCCCEEEEEEECCCCCCCHHCCCC
GEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQSWQEAAWWSEVFSYAEDRFNLPR
CCCCCCHHHHHHHHHHHCCHHEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
GTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGRWDYIFSYIKTLKNYPDRVLPDRQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCE
VTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFIPSKDEERNNQVLNKVKADKALEANNG
EECCCHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
HDGTWIAHPGLADTAMAVFNDILGSRKNQLEVMREQDAPITADQLLAPCDGERTEEGMRA
CCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCC
NIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIHHQKTLSNGKPVTKALFR
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QMLGEEMKVIASELGEERFSQGRFDDAARLMEQITTSDELIDFLTLPGYRLLA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3050899; 3060852; 8265357; 9278503 [H]