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The map label for this gene is rbsK [H]

Identifier: 218702619

GI number: 218702619

Start: 4569554

End: 4570771

Strand: Direct

Name: rbsK [H]

Synonym: ECIAI39_4374

Alternate gene names: 218702619

Gene position: 4569554-4570771 (Clockwise)

Preceding gene: 218702618

Following gene: 218702620

Centisome position: 89.04

GC content: 46.06

Gene sequence:

>1218_bases
ATGTTTGGGAAAGAAAGACAACGTCAAATATTGGCATATTTGAATAAACATGAGCGGGCTACGGTTAATCAACTCGCCAT
CTTATTCTCTGTCAGTAAAGAAACGATCCGTAATGATTTAAATTTATTAGCGGAGCAAGGGGGTGTTGAGCGTTGTCACG
GCGGAGCAATAATATTGCGTCGGATTTTCCATTCTCAGCCCGTTAATAACGTTGGTACCTCTATTGAAAAGTTCCTCAAT
CAGGTCAACTGGCGAAAAAACTCGATGACAAACAAAAAAGGGAAAATAATGATAAGCAAAGTTTGTATATTAGGATCTTT
TAATGTCGATATTATTGCCAAAGTTGATCGCTTCCCTAAAAGCGGCGAATCAATTCTTGCCAGCAATACCATCATTGGAC
CTGGCGGCAAGGGCGCAAACCAGGCGATGGCAGCCAGCAAATGTCCCGTCCAGGTACACTTCGTCGGCAAAGTTGGCAAC
GACCATTTCGGCCAGATGGCGCATGATCATCTCTCTTCATCCGATATCCGCAGCTTTACCCTCTACCAGGACGAAGAGCA
TAAAACAGGAAGCGCCATAATATATGTTTCGGAGCCTGCGGGTGAAAATAAGATCGCGATTTATTCTGGCGCCAATCAAT
ATATCACTGAAGCGGAAGTTGCCAGTATTGCGGGTGAGTTGACGGACGCCAAAATATTTTTGACACAGTTGGAAAATAAC
TTTGAAGCAACCACGCTGGCACTAAATATGGCACATCGGTCGGGCGTGAAAACTATCCTTAACCCAGCCCCTTATTCTGA
CCAGGCAAAAGAATGCCTGCAATATGTTGATTATCTGACACCGAATGAAACAGAAGCGTCGCTGATGTCCGGTGTCGAGA
TTAATGATATCGAGAGTGCGAAAGAAGCGGCGCAAACCATAAAATCCCTGGGGGCAAAAAACATCATTATTACGATGGGT
TCTCAAGGGGCGCTGGTTTATGACGGTGAACAATTTACCCATATTCCAGCGTTAAAAGCAGTAAGTATTGACACCACCGG
TGCGGGGGATGCGTTTAACGGTGCGTTTGCTGCGGCGATTGCGCAGGGCCAGCCGTTGCTCAGTGCAGCAAAATTTGCCT
CTGCGTTTGCTTCTCTGGCGGTCGAGCGGGAAGGCGCATCGAACATGCCTGAGTATGAAGCCGTACTATCACGTCTTGCT
CAATATGAGAGGAAATAA

Upstream 100 bases:

>100_bases
CCAAAAATTCCCGTTTGTTTTTGTTTTCGTGATGGTAGTGTGAAAGGATTCATGGAATCAGAAAATTACAAAAGATATAA
ATGTTACGTGAGGTGAAATC

Downstream 100 bases:

>100_bases
AATGACGAGCCAAGCCCAGAACCCATCTGATGTTTCAGGTACGATGAAGGTGGAAACCAATGGGGTAAACCCGGTTAGTG
ATAACGAACGTTATGGTTCG

Product: putative carbohydrate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MFGKERQRQILAYLNKHERATVNQLAILFSVSKETIRNDLNLLAEQGGVERCHGGAIILRRIFHSQPVNNVGTSIEKFLN
QVNWRKNSMTNKKGKIMISKVCILGSFNVDIIAKVDRFPKSGESILASNTIIGPGGKGANQAMAASKCPVQVHFVGKVGN
DHFGQMAHDHLSSSDIRSFTLYQDEEHKTGSAIIYVSEPAGENKIAIYSGANQYITEAEVASIAGELTDAKIFLTQLENN
FEATTLALNMAHRSGVKTILNPAPYSDQAKECLQYVDYLTPNETEASLMSGVEINDIESAKEAAQTIKSLGAKNIIITMG
SQGALVYDGEQFTHIPALKAVSIDTTGAGDAFNGAFAAAIAQGQPLLSAAKFASAFASLAVEREGASNMPEYEAVLSRLA
QYERK

Sequences:

>Translated_405_residues
MFGKERQRQILAYLNKHERATVNQLAILFSVSKETIRNDLNLLAEQGGVERCHGGAIILRRIFHSQPVNNVGTSIEKFLN
QVNWRKNSMTNKKGKIMISKVCILGSFNVDIIAKVDRFPKSGESILASNTIIGPGGKGANQAMAASKCPVQVHFVGKVGN
DHFGQMAHDHLSSSDIRSFTLYQDEEHKTGSAIIYVSEPAGENKIAIYSGANQYITEAEVASIAGELTDAKIFLTQLENN
FEATTLALNMAHRSGVKTILNPAPYSDQAKECLQYVDYLTPNETEASLMSGVEINDIESAKEAAQTIKSLGAKNIIITMG
SQGALVYDGEQFTHIPALKAVSIDTTGAGDAFNGAFAAAIAQGQPLLSAAKFASAFASLAVEREGASNMPEYEAVLSRLA
QYERK
>Mature_405_residues
MFGKERQRQILAYLNKHERATVNQLAILFSVSKETIRNDLNLLAEQGGVERCHGGAIILRRIFHSQPVNNVGTSIEKFLN
QVNWRKNSMTNKKGKIMISKVCILGSFNVDIIAKVDRFPKSGESILASNTIIGPGGKGANQAMAASKCPVQVHFVGKVGN
DHFGQMAHDHLSSSDIRSFTLYQDEEHKTGSAIIYVSEPAGENKIAIYSGANQYITEAEVASIAGELTDAKIFLTQLENN
FEATTLALNMAHRSGVKTILNPAPYSDQAKECLQYVDYLTPNETEASLMSGVEINDIESAKEAAQTIKSLGAKNIIITMG
SQGALVYDGEQFTHIPALKAVSIDTTGAGDAFNGAFAAAIAQGQPLLSAAKFASAFASLAVEREGASNMPEYEAVLSRLA
QYERK

Specific function: Ribose metabolism; first step. [C]

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Homo sapiens, GI11545855, Length=260, Percent_Identity=37.3076923076923, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1790193, Length=302, Percent_Identity=35.0993377483444, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI87081971, Length=282, Percent_Identity=30.1418439716312, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082343, Length=314, Percent_Identity=27.7070063694268, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788491, Length=302, Percent_Identity=25.1655629139073, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532905, Length=305, Percent_Identity=34.7540983606557, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319884, Length=331, Percent_Identity=30.5135951661631, Blast_Score=126, Evalue=6e-30,
Organism=Drosophila melanogaster, GI20128807, Length=272, Percent_Identity=37.8676470588235, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24583886, Length=286, Percent_Identity=34.965034965035, Blast_Score=121, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR011877
- InterPro:   IPR002139 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.15 [H]

Molecular weight: Translated: 43790; Mature: 43790

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00894 HTH_DEOR_1 ; PS51000 HTH_DEOR_2 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGKERQRQILAYLNKHERATVNQLAILFSVSKETIRNDLNLLAEQGGVERCHGGAIILR
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHH
RIFHSQPVNNVGTSIEKFLNQVNWRKNSMTNKKGKIMISKVCILGSFNVDIIAKVDRFPK
HHHHCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEEEEEEECCCCEEEEEECCCCC
SGESILASNTIIGPGGKGANQAMAASKCPVQVHFVGKVGNDHFGQMAHDHLSSSDIRSFT
CCCCEEECCCEECCCCCCCCHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCEEE
LYQDEEHKTGSAIIYVSEPAGENKIAIYSGANQYITEAEVASIAGELTDAKIFLTQLENN
EEECCCCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEHHCCC
FEATTLALNMAHRSGVKTILNPAPYSDQAKECLQYVDYLTPNETEASLMSGVEINDIESA
CCEEEEEEEHHHHHCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHH
KEAAQTIKSLGAKNIIITMGSQGALVYDGEQFTHIPALKAVSIDTTGAGDAFNGAFAAAI
HHHHHHHHHCCCCEEEEEECCCCCEEECCCCEEECCCEEEEEEECCCCCCCCCHHHHHHH
AQGQPLLSAAKFASAFASLAVEREGASNMPEYEAVLSRLAQYERK
HCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFGKERQRQILAYLNKHERATVNQLAILFSVSKETIRNDLNLLAEQGGVERCHGGAIILR
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHH
RIFHSQPVNNVGTSIEKFLNQVNWRKNSMTNKKGKIMISKVCILGSFNVDIIAKVDRFPK
HHHHCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEEEEEEECCCCEEEEEECCCCC
SGESILASNTIIGPGGKGANQAMAASKCPVQVHFVGKVGNDHFGQMAHDHLSSSDIRSFT
CCCCEEECCCEECCCCCCCCHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCEEE
LYQDEEHKTGSAIIYVSEPAGENKIAIYSGANQYITEAEVASIAGELTDAKIFLTQLENN
EEECCCCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEHHCCC
FEATTLALNMAHRSGVKTILNPAPYSDQAKECLQYVDYLTPNETEASLMSGVEINDIESA
CCEEEEEEEHHHHHCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHH
KEAAQTIKSLGAKNIIITMGSQGALVYDGEQFTHIPALKAVSIDTTGAGDAFNGAFAAAI
HHHHHHHHHCCCCEEEEEECCCCCEEECCCCEEECCCEEEEEEECCCCCCCCCHHHHHHH
AQGQPLLSAAKFASAFASLAVEREGASNMPEYEAVLSRLAQYERK
HCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]