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The map label for this gene is insF1 [C]

Identifier: 218702447

GI number: 218702447

Start: 4375252

End: 4375698

Strand: Direct

Name: insF1 [C]

Synonym: ECIAI39_4200

Alternate gene names: 218702447

Gene position: 4375252-4375698 (Clockwise)

Preceding gene: 218702446

Following gene: 218702448

Centisome position: 85.25

GC content: 52.57

Gene sequence:

>447_bases
GTGGCGTTCATCATTGATGTGTTTGCCGGATACATCGTGGGGGGGCGGGTATCATCGTCCATGGAAACGACATTCGTGCT
GGATGCGCTGGAGCAGGCGTTGTGGGCCCATCGTCCGTCCGGCACCATCCATCACAGCGATAAAGGCTCTCAGTATGTGT
CACTGGCCTATACGGAGCGATTAAAAGAAGCAAAACTGCTGGCATCAACAGGAAGTACAGGCGACTCGTATGACAACGCG
ATGGCGGAGAGCATCAATGGTCTTTACAAAGCGGAGGTAATACACCGTAAGAGCTGGAAAAACCGTGCAGAAGTGGAACT
GTCCACACTCACGTGGGTGGACTGGTATAACAATCGACGATTGCTGGGAAGGCTGAGGCATACTCCTCCGGCAGAAGCAG
AAAAAGCGTATTATGCTTCCATCGGAAATGATGAGCTGGCAGCCTGA

Upstream 100 bases:

>100_bases
GCTGAAAGGTGCACGTCCGGTTCGGGGAGGAGAGGCAGGGAAATAGTCCGACTACGCCCTGCCTCTTACTCTACTCAGCA
CATGGCAGGGCTTCGTCTAT

Downstream 100 bases:

>100_bases
GTTCACAGATAAAACACTCTCTAGGAACCCCGGGGCGGTTCATTCTCGCTTGCCTTCTGTATTTCAAAAAGCATCATTCA
GGTCATTAAAAGTTATTTGA

Product: transposase ORF B (fragment), IS629

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MAFIIDVFAGYIVGGRVSSSMETTFVLDALEQALWAHRPSGTIHHSDKGSQYVSLAYTERLKEAKLLASTGSTGDSYDNA
MAESINGLYKAEVIHRKSWKNRAEVELSTLTWVDWYNNRRLLGRLRHTPPAEAEKAYYASIGNDELAA

Sequences:

>Translated_148_residues
MAFIIDVFAGYIVGGRVSSSMETTFVLDALEQALWAHRPSGTIHHSDKGSQYVSLAYTERLKEAKLLASTGSTGDSYDNA
MAESINGLYKAEVIHRKSWKNRAEVELSTLTWVDWYNNRRLLGRLRHTPPAEAEKAYYASIGNDELAA
>Mature_147_residues
AFIIDVFAGYIVGGRVSSSMETTFVLDALEQALWAHRPSGTIHHSDKGSQYVSLAYTERLKEAKLLASTGSTGDSYDNAM
AESINGLYKAEVIHRKSWKNRAEVELSTLTWVDWYNNRRLLGRLRHTPPAEAEKAYYASIGNDELAA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=109, Percent_Identity=56.8807339449541, Blast_Score=118, Evalue=2e-27,
Organism=Escherichia coli, GI1788405, Length=120, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1787263, Length=120, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1786753, Length=120, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1786570, Length=120, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1786490, Length=120, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1789981, Length=139, Percent_Identity=27.3381294964029, Blast_Score=64, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 16499; Mature: 16367

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFIIDVFAGYIVGGRVSSSMETTFVLDALEQALWAHRPSGTIHHSDKGSQYVSLAYTER
CEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEHHHHHH
LKEAKLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELSTLTWVDWYNNRR
HHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCH
LLGRLRHTPPAEAEKAYYASIGNDELAA
HHHHHCCCCCCCCCHHEEEECCCCCCCC
>Mature Secondary Structure 
AFIIDVFAGYIVGGRVSSSMETTFVLDALEQALWAHRPSGTIHHSDKGSQYVSLAYTER
EEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEHHHHHH
LKEAKLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELSTLTWVDWYNNRR
HHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCH
LLGRLRHTPPAEAEKAYYASIGNDELAA
HHHHHCCCCCCCCCHHEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2159625; 2824781 [H]