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The map label for this gene is intS [H]

Identifier: 218702435

GI number: 218702435

Start: 4361791

End: 4362975

Strand: Direct

Name: intS [H]

Synonym: ECIAI39_4188

Alternate gene names: 218702435

Gene position: 4361791-4362975 (Clockwise)

Preceding gene: 218702424

Following gene: 218702436

Centisome position: 84.99

GC content: 45.15

Gene sequence:

>1185_bases
ATGGCATTAACCGATATCAAAGTGAAAACCGCAAAACCCAAAGACAAACCCTATAAGCTTGCTGATGGCGGCGGTATGTA
TCTACTGATTAACACTAATGGTTCTAAATACTGGCGCATGAAGTACCGCTTTGCTGGTAAAGAAAAGATGCTTTCTATTG
GCGTATACCCCGATGTAACGCTAGCCGATGCTCGCGAGAAACGCAGTGAAGCCAGGAAACTCCTCGCTGCTGGAGGCGAT
CCAGGGGAGGCAAAAAAGGAAGAAAAAATCGCGCAGCAAATAAGTCTGAAAAATACGTTCGAGGCTATTGCTCGCGAATG
GCACCAGTCAAAAGCCGATCGGTGGTCATTACGCTATCGTGATGAAATCATTGATACCTTTGAAAAAGACATTTTTCCCT
ACATTGGCAAGCGCCCTATTGCCGAAATCAAACCAATGGAGTTACTGGAAGCACTGCGTAAAATGGAAAAACGTGGAGCT
CTGGAGAAAATGCGCAAGGTCCGCCAGCGCTGTGGTGAGGTGTTCCGCTATGCAATTGTTACTGGCCGGGCAGACTATAA
CCCAGCCCCCGATCTTGCCAGTGCTTTAGCTACACCGAAAAAAGTACATTTTCCCTTTCTTACTGCTAATGAACTTCCCC
ACTTCCTCAACGATCTGGCGGGTTATACCGGAAGTATAATCACTAAGACAGCTACTCAGATCATTATGTTAACCGGCGTA
CGAACACAAGAACTGCGTTTTGCACGCTGGGAAGATATCGATTTTGAGACAAAATTGTGGGAGATCCCGGCAGAAGTAAT
GAAGATGAAGCGACCTCATATTGTGCCGCTCTCTGAGCAGGTTATTATGCTATTTAAGCAGCTTGAACCAATCTCAAAAC
ATCATCCCCTAGTCTTTATCGGTAGAAATGATCCCCGCAAACCAATTAGTAAGGAAAGCATTAATCAAGTAATTGAATTA
CTTGGATATAAGGGAAGACTCACAGGGCACGGTTTCAGACACACCATGAGTACAATTCTGCATGAACAAGGCTTTAACTC
CGCCTGGATTGAAATGCAGTTAGCTCATGTGGATAAAAATTCCATCAGGGGTACCTATAATCATGCCCTGTATCTCGATG
GCCGCCGTGAAATGATGCAATGGTACGCTGATTACATTGATTCACTCTCCAGCCGGGAAAGTTAA

Upstream 100 bases:

>100_bases
CAACATCTTTGTTATACAAGGGGTGTATTGGGGGTATCAGGTTAGTTACTGGCAGAAATCCCCCTCAGGAACATGAATTC
ATCAAAAGCCGGAGGATGTC

Downstream 100 bases:

>100_bases
GTCAGAAATACTTTTCCCTGATGTTTTCCTCAATTAAGTATTTTATCCTCTGGCCTACAAGGCTGGAGGATTTTTGTTAT
TATATTATTTTTTTGCGTCT

Product: putative prophage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGD
PGEAKKEEKIAQQISLKNTFEAIAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLNDLAGYTGSIITKTATQIIMLTGV
RTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIEL
LGYKGRLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSRES

Sequences:

>Translated_394_residues
MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGD
PGEAKKEEKIAQQISLKNTFEAIAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLNDLAGYTGSIITKTATQIIMLTGV
RTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIEL
LGYKGRLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSRES
>Mature_393_residues
ALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDP
GEAKKEEKIAQQISLKNTFEAIAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGAL
EKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLNDLAGYTGSIITKTATQIIMLTGVR
TQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELL
GYKGRLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSRES

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=386, Percent_Identity=48.7046632124352, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI145693166, Length=390, Percent_Identity=42.5641025641026, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI1788974, Length=391, Percent_Identity=39.6419437340153, Blast_Score=290, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45430; Mature: 45299

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRFAGKEKMLSIGVYPDVT
CCCCCEEEECCCCCCCCEEEECCCCEEEEEECCCCEEEEEEEEECCCHHEEEECCCCCCC
LADAREKRSEARKLLAAGGDPGEAKKEEKIAQQISLKNTFEAIAREWHQSKADRWSLRYR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
DEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGALEKMRKVRQRCGEVFRYAIV
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TGRADYNPAPDLASALATPKKVHFPFLTANELPHFLNDLAGYTGSIITKTATQIIMLTGV
CCCCCCCCCHHHHHHHCCCCEEECCEEEHHHHHHHHHHHCCCCCHHHHHHHHHEEEEECC
RTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFI
CHHHHHHHCCCCCCCCHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHCCHHCCCCEEEE
GRNDPRKPISKESINQVIELLGYKGRLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKN
ECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHEEEEEECCCCH
SIRGTYNHALYLDGRREMMQWYADYIDSLSSRES
HCCCEECEEEEECCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRFAGKEKMLSIGVYPDVT
CCCCEEEECCCCCCCCEEEECCCCEEEEEECCCCEEEEEEEEECCCHHEEEECCCCCCC
LADAREKRSEARKLLAAGGDPGEAKKEEKIAQQISLKNTFEAIAREWHQSKADRWSLRYR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
DEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGALEKMRKVRQRCGEVFRYAIV
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TGRADYNPAPDLASALATPKKVHFPFLTANELPHFLNDLAGYTGSIITKTATQIIMLTGV
CCCCCCCCCHHHHHHHCCCCEEECCEEEHHHHHHHHHHHCCCCCHHHHHHHHHEEEEECC
RTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFI
CHHHHHHHCCCCCCCCHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHCCHHCCCCEEEE
GRNDPRKPISKESINQVIELLGYKGRLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKN
ECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHEEEEEECCCCH
SIRGTYNHALYLDGRREMMQWYADYIDSLSSRES
HCCCEECEEEEECCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]