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The map label for this gene is trmH

Identifier: 218702420

GI number: 218702420

Start: 4342597

End: 4343286

Strand: Direct

Name: trmH

Synonym: ECIAI39_4173

Alternate gene names: 218702420

Gene position: 4342597-4343286 (Clockwise)

Preceding gene: 218702419

Following gene: 218702421

Centisome position: 84.62

GC content: 55.07

Gene sequence:

>690_bases
ATGAACCCAACACGTTATGCACGTATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGT
CCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGTGTACATGAAGTTCATGCTGTCTGGCCTG
GTAGCCGGATGCGCACCATGGCTTCGGCAGCTGCGGGTAGTAACAGCTGGGTACAGGTGAAAACGCACCGCACCATTGGC
GATGCCGTCGCTCATCTGAAAGGCCAGGGCATGCAGATTCTGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGA
AATTGATTACACTCGCCCGACCTGTATTTTGATGGGACAGGAGAAAACAGGCATCACGCAGGAAGCGTTGGCCCTGGCGG
ATCGGGACATCATCATTCCGATGATTGGCATGGTGCAATCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAA
GCCCAGCGCCAGCGGCAAAATGCAGGCATGTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGCT
GTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGTCTGCCCTACCCCCACGTTAATCAGCAAGGCG
AGATTGAAGCCGATGCCGACTGGTGGTCCACCATGCAGGCCGCAGGGTAA

Upstream 100 bases:

>100_bases
TCTGACCGCCCGTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCC
GAAACCGAAATTAATGTTTT

Downstream 100 bases:

>100_bases
GCGCGATGAAAGGTCGCCTGTTAGATGCTGTCCCGCTCAGTTCCCTAACGGGCGTTGGCGCAGCGCTTAGTAACAAGCTG
GCGAAAATCAACCTGCATAC

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIG
DAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWSTMQAAG

Sequences:

>Translated_229_residues
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIG
DAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWSTMQAAG
>Mature_229_residues
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIG
DAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWSTMQAAG

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=229, Percent_Identity=99.1266375545851, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1790623, Length=160, Percent_Identity=31.875, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25388; Mature: 25388

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTM
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHEEEHHHHCCHHHEEEECCCHHHHHH
ASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQ
HHHHCCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEHHCCCCCCHHHCCCCCCEEEEEEC
EKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPE
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
AEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWSTMQAAG
HHHHHEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTM
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHEEEHHHHCCHHHEEEECCCHHHHHH
ASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQ
HHHHCCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEHHCCCCCCHHHCCCCCCEEEEEEC
EKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPE
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
AEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWSTMQAAG
HHHHHEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]