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The map label for this gene is tiaE

Identifier: 218702318

GI number: 218702318

Start: 4231162

End: 4232136

Strand: Direct

Name: tiaE

Synonym: ECIAI39_4061

Alternate gene names: 218702318

Gene position: 4231162-4232136 (Clockwise)

Preceding gene: 218702317

Following gene: 218702320

Centisome position: 82.45

GC content: 51.59

Gene sequence:

>975_bases
ATGAAGCCGTCCGTTATCCTCTACAAAGCCTTACCTGATGATTTACTGCAACGCCTGCAAGAACATTTCACCGTTCACCA
GGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGCTTGCTGGGTTCAAATG
AGAATGTTGATGCCGTATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTT
GATGTCGATGCGCTTACCGCCCGAAAAATTCTGCTGATGCACACACCAACCGTATTAACAGAAACCGTCGCCGATACGCT
GATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAGCAGGCGAATGGACCGCGAGCA
TAGGCCCGGACTGGTACGGCACTGACGTTCACCATAAAACACTGGGCATTGTCGGGATGGGACGGATCGGCATGGCGCTA
GCACAACGTGCGCACTTTGGCTTCAACATGCCCATCCTCTATAACGCGCGTCGCCACCATAAAGAAGCAGAAGAACGCTT
CAACGCCCGCTACTGCGATTTGGATACTCTGTTACAAGAGTCAGATTTCGTTTGCCTGATCCTGCCGTTAACGGATGAGA
CGCATCATCTGTTTGGCGCAGAACAATTCGCCAAAATGAAATCCTCCGCCATTTTCATTAATGCCGGACGTGGCCCGGTG
GTTGATGAAAATGCACTGATTGCTGCATTGCAGAAAGGGGAAATTCACGCCGCCGGGCTGGATGTCTTCGAACAAGAGCC
ACTTTCCGTAGATTCGCCGTTGCTCTCAATGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGC
GTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCG
CACGTCGCGGACTAA

Upstream 100 bases:

>100_bases
CTTTCATGTCAGGTGATGCAGAAATCACCTGGCATTCTTCAGTTTTCCTTCATCATATTTCAGGCTAAGGTGATCGCCTT
ATCAGTGAATGGAGAGAAGC

Downstream 100 bases:

>100_bases
GCCGCGACTGCGTGGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAG
GCCTGATTAAACGCCTGATG

Product: 2-oxo-carboxylic acid reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAVLLEKMPKLRATSTISVGYDNF
DVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNP
HVAD

Sequences:

>Translated_324_residues
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAVLLEKMPKLRATSTISVGYDNF
DVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNP
HVAD
>Mature_324_residues
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAVLLEKMPKLRATSTISVGYDNF
DVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNP
HVAD

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=273, Percent_Identity=42.1245421245421, Blast_Score=191, Evalue=8e-49,
Organism=Homo sapiens, GI23308577, Length=312, Percent_Identity=30.7692307692308, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI145580578, Length=268, Percent_Identity=32.089552238806, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI4557499, Length=268, Percent_Identity=32.089552238806, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI145580575, Length=274, Percent_Identity=32.4817518248175, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI61743967, Length=270, Percent_Identity=32.2222222222222, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI4557497, Length=270, Percent_Identity=32.2222222222222, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI87082289, Length=324, Percent_Identity=99.3827160493827, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI1787645, Length=269, Percent_Identity=27.5092936802974, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789279, Length=237, Percent_Identity=31.6455696202532, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081824, Length=258, Percent_Identity=27.1317829457364, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=250, Percent_Identity=33.6, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25147481, Length=274, Percent_Identity=30.2919708029197, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=243, Percent_Identity=38.6831275720165, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320925, Length=248, Percent_Identity=33.0645161290323, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=248, Percent_Identity=31.4516129032258, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6325144, Length=196, Percent_Identity=31.6326530612245, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=240, Percent_Identity=27.0833333333333, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6321253, Length=279, Percent_Identity=24.7311827956989, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45552429, Length=312, Percent_Identity=39.1025641025641, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24585514, Length=312, Percent_Identity=39.1025641025641, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI28574282, Length=312, Percent_Identity=39.1025641025641, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI28574284, Length=312, Percent_Identity=39.1025641025641, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45551003, Length=312, Percent_Identity=39.1025641025641, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI28574286, Length=315, Percent_Identity=37.7777777777778, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI28571528, Length=261, Percent_Identity=41.7624521072797, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24585516, Length=247, Percent_Identity=36.0323886639676, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24646446, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646448, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646452, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646450, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI62472511, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19921140, Length=251, Percent_Identity=30.6772908366534, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35425; Mature: 35425

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAVL
CCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCCCCCCCHHH
LEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVE
HHHCCCCCCCEEEEECCCCCCCHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYNARRHH
HHHHHHCCCEEECCCCCCCCCCCCHHHCCEEECCHHHHHHHHHHHCCCCCCEEECCHHHH
KEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
HHHHHHHCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHCHHHHHHHCCCEEEEECCCCCC
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAAC
CCCCHHEEEHHCCCEEECCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AVDNLIDALQGKVEKNCVNPHVAD
HHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAVL
CCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCCCCCCCHHH
LEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVE
HHHCCCCCCCEEEEECCCCCCCHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYNARRHH
HHHHHHCCCEEECCCCCCCCCCCCHHHCCEEECCHHHHHHHHHHHCCCCCCEEECCHHHH
KEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
HHHHHHHCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHCHHHHHHHCCCEEEEECCCCCC
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAAC
CCCCHHEEEHHCCCEEECCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AVDNLIDALQGKVEKNCVNPHVAD
HHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA