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The map label for this gene is yhiF

Identifier: 218702263

GI number: 218702263

Start: 4156990

End: 4157520

Strand: Direct

Name: yhiF

Synonym: ECIAI39_4000

Alternate gene names: 218702263

Gene position: 4156990-4157520 (Clockwise)

Preceding gene: 218702262

Following gene: 218702266

Centisome position: 81.0

GC content: 40.11

Gene sequence:

>531_bases
ATGTTTCTTATAATTACCAGGGATACGATGTTCTTCACCGCGATGAAAAAAATTCTGAGTAAAGGTAATGTCGTTCATAT
ACAGAACGAAGAAGAGATCGACGTAATGTTGCATCAGCATGCCTTCGTCATTATTGATACATTAATGAATAATGTATTTC
ATTCTAATTTGCTCACTCAGATTGAACGATTAAAACCTGTCCATGTGATTATTTTCTCTCCCTTTAATATTAAACGCTGC
CTGGGGAAAGTACCGGTGACCTTTGTTCCGCGAACTATCACCATCATCGACTTTGTCGCACTCATCAATGGCAGTTACTG
CTCTGTGCCTGAAGCGGATGTGTCCCTTTCGCGCAAGCAGCATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGG
AAGATATTCTGGAGAAACTGAAAATATCGCTAAAAACGTTCTACTGCCATAAACACAATATCATGATGATCCTCAATCTT
AAGCGGATCAATGAGTTGGTACGCCATCAGCATATTGATTATCTGGTGTGA

Upstream 100 bases:

>100_bases
AAATTAATACACAGCCAGCTAACTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAAT
GTCTGCACCAGGAGTCGGTT

Downstream 100 bases:

>100_bases
TCGATTGATTAATATAAAGAGGCCCAGCAATAAATAGCCAGACCTCCCTTTAATTATACGTTATGCAGTAACGCCTTCCG
GTATCAGCGAAGCAATTTGC

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MFLIITRDTMFFTAMKKILSKGNVVHIQNEEEIDVMLHQHAFVIIDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRC
LGKVPVTFVPRTITIIDFVALINGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
KRINELVRHQHIDYLV

Sequences:

>Translated_176_residues
MFLIITRDTMFFTAMKKILSKGNVVHIQNEEEIDVMLHQHAFVIIDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRC
LGKVPVTFVPRTITIIDFVALINGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
KRINELVRHQHIDYLV
>Mature_176_residues
MFLIITRDTMFFTAMKKILSKGNVVHIQNEEEIDVMLHQHAFVIIDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRC
LGKVPVTFVPRTITIIDFVALINGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
KRINELVRHQHIDYLV

Specific function: May act as a transcriptional regulator of dctA. Could be involved in the regulation of the genes coding for the type III secretion system in enterohaemorragic strains [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH luxR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789923, Length=176, Percent_Identity=97.7272727272727, Blast_Score=349, Evalue=5e-98,
Organism=Escherichia coli, GI1789928, Length=167, Percent_Identity=23.9520958083832, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016032
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE [H]

EC number: NA

Molecular weight: Translated: 20456; Mature: 20456

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLIITRDTMFFTAMKKILSKGNVVHIQNEEEIDVMLHQHAFVIIDTLMNNVFHSNLLTQ
CEEEEECCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALINGSYCSVPEADVSLSRKQ
HHHCCCEEEEEECCCCHHHHHCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
HQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNLKRINELVRHQHIDYLV
HHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCEEEEEEHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFLIITRDTMFFTAMKKILSKGNVVHIQNEEEIDVMLHQHAFVIIDTLMNNVFHSNLLTQ
CEEEEECCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALINGSYCSVPEADVSLSRKQ
HHHCCCEEEEEECCCCHHHHHCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
HQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNLKRINELVRHQHIDYLV
HHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCEEEEEEHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]