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The map label for this gene is insG

Identifier: 218702000

GI number: 218702000

Start: 3886772

End: 3888118

Strand: Direct

Name: insG

Synonym: ECIAI39_3726

Alternate gene names: 218702000

Gene position: 3886772-3888118 (Clockwise)

Preceding gene: 218701999

Following gene: 218702002

Centisome position: 75.74

GC content: 53.53

Gene sequence:

>1347_bases
TTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGAC
TTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGTAACGCTACGCAAGCGCC
GTCTTCCCCTCGAAATGATGGTCTGGTGTATTGTTGGCATGGCGCTTGAGCGTAAAGAACCTCTTCACCAGATTGTGAAT
CGCCTGGACATCATGCTGCCGGGCAATCGCCCCTTCGTTGCCCCCAGTGCCGTTATTCAGGCCCGCCAGCGCCTGGGAAG
TGAGGCTGTCCGCCGCGTGTTCACGAAAACAGCGCAGCTCTGGCATAACGCCACGCCGCATCCGCACTGGTGCGGCCTGA
CCCTGCTGGCCATCGATGGTGTGTTCTGGCGCACACCGGATACACCAGAGAACGATGCAGCCTTCCCCCGCCAGACACAT
GCCGGGAACCCGGCGCTCTACCCGCAGGTCAAAATGGTCTGCCAGATGGAACTGACCAGCCATCTGCTGACGGCTGCAGC
CTTCGGCACGATGAAGAACAGCGAAAATGAGCTTGCTGAGCAACTTATAGAACAAACCGGCGATAACACTCTGACGTTAA
TGGATAAAGGTTATTACTCACTGGGACTGTTAAATGCCTGGAGCCTGGCGGGAGAACACCGCCACTGGATGATACCTCTC
AGAAAGGGAGCGCAATATGAAGAGATCAGAAAACTGGGTAAAGGCGATCATCTGGTGAAGCTGAAAACCAGCCCGCAGGC
ACGAAAAAAGTGGCCGGGACTGGGAAATGAAGTGACAGCCCGCCTGCTGACCGTGACGCGCAAAGGAAAAGTCTGCCATC
TGCTGACGTCGATGACGGACGCCATGCGCTTCCCCGGAGGAGAAATGGCGGATCTGTACAGTCATCGCTGGGAAATCGAA
CTGGGATACAGGGAGATAAAACAGACGATGCAACTGAGCAGGCTGACGCTGAGAAGTAAAAAACCGGAGCTTGTGGAGCA
AGAGCTGTGGGGTGTCTTACTGGCTTATAATCTGGTGAGATATCAGATGATTAAAATGGCAGAACATCTGAAAGGTTACT
GGCCGAATCAACTGAGTTTCTCAGAATCATGTGGAATGGTGATGAGAATGCTGATGACATTGCAGGGCGCTTCACCGGGA
CGTATACCGGAGCTGATGCGCGATCTTGCAAGTATGGGACAACTTGTGAAATTACCGACAAGAAGGGAAAGGGCCTTCCC
GAGAGTGGTAAAGGAGAGGCCCTGGAAATACCCCACAGCCCCGAAAAAGAGCCAGTCAGTTGCTTAA

Upstream 100 bases:

>100_bases
GTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGT
AAGAATCCGGAAACGCTCAC

Downstream 100 bases:

>100_bases
CTGACTGGCATTAGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGC
GATATCTTTCTTCAGCGTAT

Product: putative IS4 transposase; KpLE2 phage-like element

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVN
RLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPL
RKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIE
LGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPG
RIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA

Sequences:

>Translated_448_residues
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVN
RLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPL
RKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIE
LGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPG
RIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA
>Mature_448_residues
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVN
RLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPL
RKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIE
LGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPG
RIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=442, Percent_Identity=99.5475113122172, Blast_Score=910, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 51143; Mature: 51143

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMM
CCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCEEEHHHCCCHHHH
VWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQL
HHHHHHHHHHHCCHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTS
HHCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPL
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEC
RKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTD
CCCCCHHHHHHHCCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
AMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVR
HHHCCCCCHHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPT
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
RRERAFPRVVKERPWKYPTAPKKSQSVA
HHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMM
CCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCEEEHHHCCCHHHH
VWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQL
HHHHHHHHHHHCCHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTS
HHCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPL
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEC
RKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTD
CCCCCHHHHHHHCCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
AMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVR
HHHCCCCCHHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPT
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
RRERAFPRVVKERPWKYPTAPKKSQSVA
HHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]