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The map label for this gene is dacB

Identifier: 218701951

GI number: 218701951

Start: 3838727

End: 3840160

Strand: Direct

Name: dacB

Synonym: ECIAI39_3677

Alternate gene names: 218701951

Gene position: 3838727-3840160 (Clockwise)

Preceding gene: 218701949

Following gene: 218701956

Centisome position: 74.8

GC content: 53.0

Gene sequence:

>1434_bases
ATGCGATTTTCCAGATTTATCATCGGATTGACCAGCTGTATAGCGTTCAGTGTTCAGGCCGCAAATGTTGATGAGTACAT
TACTCAACTCCCCGCTGGTGCCAACCTTGCCCTGATGGTGCAAAAAGTCGGCGCGTCGGCCCCCGCTATTGATTACCACA
GTCAGCAGATGGCGCTGCCCGCCAGCACGCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTT
CGTTTTACCACGACGCTTGAAACCAAAGGTAATGTGGAAAACGGTGTACTTAAGGGTGACTTAGTAGCGCGATTTGGTGC
CGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGATGGCA
ATGTGTTGATAGATACCTCCATTTTCGCCAGCCACGATAAAGCCCCCGGCTGGCCATGGAATGACATGACACAATGCTTT
AGCGCTCCGCCTGCCGCCGCCATAGTTGACCGCAACTGTTTCTCCGTCTCGCTCTATAGTGCCCCAAAACCTGGTGATAT
GGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAG
CGCAATACTGCGAGCTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACCGGATGCCTGCCACAACGTTCTGAG
CCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGTGCCAGCTATGCTGGAGCAATTCTGAAAGATGAGTTAAAACAGGCAGG
TATCACCTGGAGCGGAACACTGCTACGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCC
CGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATTGCCGATACGGTTTTCCGCATGATCGGCCAT
GCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCAGCAAGCCGGTGTCGA
TATTGGTAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGATTGCCCCCGCCACCATGATGCAGGTGC
TGCAATACATAGCCCAACACGACAATGAACTTAACTTTATCTCTATGCTGCCGCTGGCGGGCTATGACGGCTCTTTGCAG
TACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGCAAAGTCTCAGCGAAAACCGGCTCGTTGCAGGGGGTATATAACCT
GGCGGGATTCATTACCACGGCGAGCGGGCAACGAATGGCGTTTGTACAATATCTTTCTGGCTATGCAGTAGAACCGGCGG
ATCAGCGTAATCGCCGTATTCCGTTGGTACGTTTTGAAAGCCGTTTGTATAAAGATATTTACCAGAACAATTAG

Upstream 100 bases:

>100_bases
ATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGATTC
CAGGTTGTTAGCGCGAGATT

Downstream 100 bases:

>100_bases
TCTAAAGAGAAACCCCGGCGCATGGCCGGGGTTTTGCTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCT
TCGTCCCAGTCGTCATCCCA

Product: D-alanyl-D-alanine carboxypeptidase/endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVGASAPAIDYHSQQMALPASTQKVITALAALIQLGPDF
RFTTTLETKGNVENGVLKGDLVARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCELDVVPGDLNRFTLTGCLPQRSE
PLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLRQTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGH
ARFNVPGTWRAGSDAVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPLAGYDGSLQ
YRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAVEPADQRNRRIPLVRFESRLYKDIYQNN

Sequences:

>Translated_477_residues
MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVGASAPAIDYHSQQMALPASTQKVITALAALIQLGPDF
RFTTTLETKGNVENGVLKGDLVARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCELDVVPGDLNRFTLTGCLPQRSE
PLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLRQTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGH
ARFNVPGTWRAGSDAVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPLAGYDGSLQ
YRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAVEPADQRNRRIPLVRFESRLYKDIYQNN
>Mature_477_residues
MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVGASAPAIDYHSQQMALPASTQKVITALAALIQLGPDF
RFTTTLETKGNVENGVLKGDLVARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCELDVVPGDLNRFTLTGCLPQRSE
PLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLRQTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGH
ARFNVPGTWRAGSDAVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPLAGYDGSLQ
YRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAVEPADQRNRRIPLVRFESRLYKDIYQNN

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family

Homologues:

Organism=Escherichia coli, GI1789573, Length=477, Percent_Identity=100, Blast_Score=986, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DACB_ECOLI (P24228)

Other databases:

- EMBL:   X59460
- EMBL:   X60038
- EMBL:   U01376
- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A54535
- RefSeq:   AP_003725.1
- RefSeq:   NP_417649.1
- PDB:   2EX2
- PDB:   2EX6
- PDB:   2EX8
- PDB:   2EX9
- PDB:   2EXA
- PDB:   2EXB
- PDBsum:   2EX2
- PDBsum:   2EX6
- PDBsum:   2EX8
- PDBsum:   2EX9
- PDBsum:   2EXA
- PDBsum:   2EXB
- ProteinModelPortal:   P24228
- SMR:   P24228
- IntAct:   P24228
- STRING:   P24228
- MEROPS:   S13.001
- EnsemblBacteria:   EBESCT00000003389
- EnsemblBacteria:   EBESCT00000018009
- GeneID:   947693
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3149
- KEGG:   eco:b3182
- EchoBASE:   EB0198
- EcoGene:   EG10202
- eggNOG:   COG2027
- GeneTree:   EBGT00050000010654
- HOGENOM:   HBG432869
- OMA:   MLLKSDN
- ProtClustDB:   PRK11113
- BioCyc:   EcoCyc:EG10202-MONOMER
- DrugBank:   DB00303
- DrugBank:   DB00760
- Genevestigator:   P24228
- GO:   GO:0006508
- InterPro:   IPR012338
- InterPro:   IPR000667
- PRINTS:   PR00922
- TIGRFAMs:   TIGR00666

Pfam domain/function: PF02113 Peptidase_S13; SSF56601 PBP_transp_fold

EC number: =3.4.16.4

Molecular weight: Translated: 51799; Mature: 51799

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: ACT_SITE 62-62 ACT_SITE 65-65 ACT_SITE 306-306 BINDING 417-417

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVGASAPAIDYHSQQMALP
CCHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCCCCEEHEEHHCCCCCEECCCCCCEECC
ASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLVARFGADPTLKRQDIRNMV
CHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECHHHHHCCCCCCHHHHHHHHHH
ATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCFSAPPAAAIVDRNCFSVSLYS
HHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHCCCCCHHEECCCCEEEEEEC
APKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCELDVVPGDLNRFTLTGCLPQRSE
CCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCC
PLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLRQTQVNEPGTVVASKQSAPLHDLLK
CCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCCCCHHHHHH
IMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSDAVRQILRQQAGVDIGNTIIADGSGLS
HHHHCCCCHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCC
RHNLIAPATMMQVLQYIAQHDNELNFISMLPLAGYDGSLQYRAGLHQAGVDGKVSAKTGS
CCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEHHCCCHHCCCCCEEECCCCC
LQGVYNLAGFITTASGQRMAFVQYLSGYAVEPADQRNRRIPLVRFESRLYKDIYQNN
CCHHHHHHHEEEECCCCHHHHHHHHCCCCCCCCHHCCCCCCEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVGASAPAIDYHSQQMALP
CCHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCCCCEEHEEHHCCCCCEECCCCCCEECC
ASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLVARFGADPTLKRQDIRNMV
CHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECHHHHHCCCCCCHHHHHHHHHH
ATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCFSAPPAAAIVDRNCFSVSLYS
HHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHCCCCCHHEECCCCEEEEEEC
APKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCELDVVPGDLNRFTLTGCLPQRSE
CCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCC
PLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLRQTQVNEPGTVVASKQSAPLHDLLK
CCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCCCCHHHHHH
IMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSDAVRQILRQQAGVDIGNTIIADGSGLS
HHHHCCCCHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCC
RHNLIAPATMMQVLQYIAQHDNELNFISMLPLAGYDGSLQYRAGLHQAGVDGKVSAKTGS
CCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEHHCCCHHCCCCCEEECCCCC
LQGVYNLAGFITTASGQRMAFVQYLSGYAVEPADQRNRRIPLVRFESRLYKDIYQNN
CCHHHHHHHEEEECCCCHHHHHHHHCCCCCCCCHHCCCCCCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551